LOCUS       V032383                 8520 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V032383
VERSION     V032383
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             613..1329
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             1384..5175
                     /label="VARS1(NM_006295)"
                     /note="VARS1(NM_006295)"
                     /gene="VARS1"
     polyA_signal    5308..5429
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(5436..5891)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5918..6022
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        6024..6381
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             6416..7210
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    7442..7489
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      7818..8406
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgaggc ggcggaggga gtggaggagg gggaagcaag
     1381 cttatgtcca ccctctacgt ctcccctcac ccagatgcct tccccagcct ccgagccctc
     1441 atagccgctc gctatgggga ggctggggag ggtcccggat ggggaggagc ccacccccgc
     1501 atctgtctcc agccaccccc gactagcagg actccctttc ccccaccccg cctgccggcc
     1561 ctggagcagg ggcccggtgg gctctgggtg tggggggcca cggctgtggc ccagctgctg
     1621 tggccagcag gcctgggggg cccagggggc agccgggcgg ctgtccttgt ccaacagtgg
     1681 gtcagttacg ccgacacgga gttaatacca gctgcctgtg gagcaacgct gccggccctg
     1741 ggactccgaa gctcggccca ggacccccag gctgtgctgg gggccctggg cagggccctg
     1801 agccccttgg aggagtggct tcggctgcac acctacttgg ccggggaggc ccccactctg
     1861 gctgacctgg cggctgtcac agccttgctg ctgcctttcc gatacgtcct agacccacct
     1921 gcccgccgga tctggaataa tgtgactcgc tggtttgtca cgtgtgtccg gcagccagaa
     1981 ttccgagccg tgctaggaga agtggttcta tactcaggag ccaggcctct ctctcatcag
     2041 ccaggccccg aggctcctgc cctcccaaag acagctgctc agctcaagaa agaggcaaag
     2101 aaacgggaga agctagagaa attccaacag aagcagaaga tccaacagca gcagccacct
     2161 ccaggggaga agaaaccaaa accagagaag agggagaaac gggatcctgg ggtcattacc
     2221 tatgacctcc caaccccacc cggggaaaag aaagatgtca gtggccccat gcccgactcc
     2281 tacagccctc ggtatgtgga ggctgcctgg tacccttggt gggagcagca gggcttcttc
     2341 aagccagagt atgggcgtcc taatgtgtca gcagcaaatc cccgaggtgt cttcatgatg
     2401 tgcatcccac cccccaatgt gacaggctcc ctgcacctgg gccatgcact caccaacgcc
     2461 atccaggact ccctgactcg atggcaccgc atgcgtgggg agaccaccct gtggaaccct
     2521 ggctgtgacc atgcaggtat tgccacccag gtggtggtgg agaagaagct atggcgtgag
     2581 cagggactga gccggcacca gctgggccgc gaggcctttc tacaggaagt ctggaagtgg
     2641 aaggaggaga aaggtgaccg gatttaccac cagttgaaga agcttggcag ctccttggac
     2701 tgggatcgag cctgtttcac catggaccct aaactctcag cagctgtgac agaggccttt
     2761 gtccggcttc acgaggaagg catcatctat cgcagtaccc gccttgttaa ctggtcctgc
     2821 accctcaact ccgccatctc tgacattgag gtggataaga aggagctgac aggtcgcacc
     2881 ctgctctccg tgcctggcta caaggagaag gtggagttcg gggtcctcgt gtcctttgcc
     2941 tataaggtcc aaggctcaga tagcgacgag gaggtggtgg tggcaacaac tcggatcgag
     3001 acaatgctgg gagatgtggc tgtagctgtg caccccaaag ataccagata ccagcacctg
     3061 aaggggaaga acgtgatcca cccattcctg tctcggagcc ttcccattgt cttcgatgaa
     3121 tttgtggaca tggactttgg cacaggtgct gtgaagatca cccccgcaca tgaccaaaat
     3181 gactatgaag ttgggcagcg gcacgggctg gaggccatca gcatcatgga ctcccggggg
     3241 gccctcatca atgtgcctcc gcctttcctg ggcctgccca ggtttgaggc caggaaagcg
     3301 gtgctggtgg cgctgaagga gcggggactg ttccgtggca ttgaggacaa ccccatggtg
     3361 gtgccacttt gcaaccggtc gaaggacgtg gtagagcctc tgctgcggcc gcagtggtac
     3421 gttcgctgcg gggagatggc ccaggctgcc agcgccgctg tgactcgggg tgacctccgc
     3481 atcctgcctg aggcccatca gcgcacatgg catgcctgga tggacaacat ccgggagtgg
     3541 tgcatttcca ggcagctgtg gtggggccat cgcatcccag cctactttgt cactgtcagt
     3601 gacccagcgg tgccccctgg ggaggaccct gatgggcggt actgggtgag tggacgcaat
     3661 gaggcggagg cccgggagaa ggcagccaag gagttcggag tgtcccctga caagatcagt
     3721 ctccagcaag atgaggatgt attggatacc tggttctcct ctggcctctt ccccttatcc
     3781 attttgggct ggcccaacca gtcagaagac ctgagtgtgt tctaccccgg gacactgctg
     3841 gagaccggtc atgacatcct cttcttctgg gtggcccgga tggtcatgct gggcctgaag
     3901 ctcacgggca ggctgccctt tagagaggtc tacctccatg ccatcgtgcg agatgctcac
     3961 ggccggaaga tgagcaagtc tctaggcaat gtcatcgatc ccctggacgt catctatgga
     4021 atctccctgc agggcctcca caaccagctg ctgaacagca acctggatcc cagcgaggtg
     4081 gagaaggcca aagaagggca gaaagctgac ttcccagcgg ggattcctga atgtggcacc
     4141 gatgctctcc ggtttggatt atgtgcctac atgtcccagg gtcgtgacat caacctggat
     4201 gtgaaccgga tactgggtta ccgccacttc tgcaacaagc tctggaatgc caccaagttt
     4261 gcccttcgtg gccttgggaa gggttttgtg ccctcaccca cctcccagcc cggaggccat
     4321 gagagcctgg tggaccgctg gatccgcagc cgcctgacag aggctgtgag gctcagcaat
     4381 caaggcttcc aggcctacga cttcccggcc gtcaccactg cccagtacag cttctggctc
     4441 tatgagctct gtgatgtcta cttggagtgc ctgaaacctg tactgaatgg ggtggaccag
     4501 gtggcagctg agtgtgcccg ccagaccctg tacacttgcc tggacgttgg cctgcggctg
     4561 ctctcaccct tcatgccctt cgtgacggag gagctgttcc agaggctgcc ccggaggatg
     4621 ccgcaagctc cccctagcct ctgtgttacc ccctacccgg agccctcaga gtgctcctgg
     4681 aaggaccccg aggcagaagc cgcccttgag ctggcgctaa gcatcacgcg agccgtgcgc
     4741 tccctgcggg ccgactacaa cctcacccgg atccggcctg actgtttcct ggaagtggcg
     4801 gatgaggcca cgggcgccct ggcatcggcg gtgtcgggct acgtgcaggc cctggccagc
     4861 gcaggtgtgg tggctgttct ggccctgggg gctcccgccc cccagggttg cgctgtggct
     4921 ctggcttctg atcgctgctc catccacctg cagcttcagg ggctggtgga ccctgcacgg
     4981 gagctgggca agctgcaagc caagcgagtt gaggcccagc ggcaggccca gcgtctgcgg
     5041 gaacgccgtg ctgcctcggg ctatcctgtc aaggtgccgc tcgaagtcca ggaggcagat
     5101 gaagccaagc tccaacagac agaagcagag ctcaggaagg tggatgaggc catcgcccta
     5161 ttccagaaga tgctgtgagg atccaccgga tctagataac tgatcataat cagccatacc
     5221 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
     5281 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     5341 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     5401 ggtttgtcca aactcatcaa tgtatcttaa cgcgtaaatt gtaagcgtta atattttgtt
     5461 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     5521 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     5581 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     5641 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
     5701 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     5761 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     5821 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     5881 acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     5941 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     6001 ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag
     6061 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     6121 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     6181 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     6241 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     6301 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     6361 ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat
     6421 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     6481 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     6541 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga
     6601 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     6661 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     6721 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     6781 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     6841 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     6901 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga
     6961 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     7021 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     7081 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     7141 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     7201 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
     7261 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     7321 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     7381 cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg
     7441 acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg
     7501 gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac
     7561 gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc
     7621 gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag
     7681 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     7741 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     7801 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     7861 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     7921 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     7981 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     8041 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     8101 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     8161 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     8221 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     8281 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     8341 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     8401 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     8461 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat
//
