LOCUS       V032875                 7134 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V032875
VERSION     V032875
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             613..1329
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             1384..3789
                     /label="TARS3(NM_152334)"
                     /note="TARS3(NM_152334)"
                     /gene="TARS3"
     polyA_signal    3922..4043
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(4050..4505)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4532..4636
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        4638..4995
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5030..5824
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6056..6103
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      6432..7020
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgaggc ggcggaggga gtggaggagg gggaagcaag
     1381 cttatggcgg ccgaggccct ggcggcggag gccgtggcgt cgcgcctgga gcggcaggag
     1441 gaggacatcc gctggctgtg gtcggaggtc gagcgcctga gggacgagca gctgaacgcg
     1501 ccctacagct gccaggcgga ggggccgtgc ctcacgcggg aggtggcgca gctccgggcc
     1561 gagaactgcg acctgcgcca ccgcctgtgc agcctgcggc tgtgcctcgc cgaggagcgg
     1621 agccgccagg ccacgctgga gagcgcggag ctagaggcgg cgcaggaggc cggcgcacag
     1681 cctcctccta gtcaaagcca agacaaggac atgaaaaaga agaaaatgaa ggaaagcgag
     1741 gctgacagcg aggtgaagca tcaaccaatt ttcataaaag aaagattgaa gctttttgaa
     1801 atactgaaga aagaccatca gctcttactt gccatttatg gaaaaaaggg ggatacaagc
     1861 aacatcatca cagtaagagt ggctgatggg caaacagtgc aaggggaagt ctggaaaaca
     1921 acgccttacc aagtggctgc tgaaattagt caggaactgg ctgaaagcac ggtaatagcc
     1981 aaagtcaatg gtgaactgtg ggacctggac cgcccattgg aaggggactc ttctctagag
     2041 ctgcttacat ttgataatga ggaagctcaa gctgtgtact ggcactccag tgctcacatt
     2101 cttggggagg ccatggagct ttactatgga ggccacctgt gctacggtcc gcccattgaa
     2161 aatggatttt attatgacat gttcattgaa gacagagcag tgtccagcac agaattgtca
     2221 gccctggaga atatatgtaa agccatcata aaagaaaagc aaccttttga aagactagaa
     2281 gtcagcaagg aaatcctcct ggaaatgttt aagtacaata aatttaaatg ccgcattctg
     2341 aatgagaaag ttaacactgc aactaccacc gtgtacaggt gcggtccatt aattgacctt
     2401 tgcaaaggtc cacatgtaag acacactgga aaaattaaaa ccatcaaaat ttttaagaat
     2461 tcctcaacat attgggaggg caatccggaa atggaaacat tgcagaggat ctatggaata
     2521 tcctttcctg ataacaagat gatgagagac tgggaaaagt tccaagagga agcaaagaac
     2581 cgagatcaca ggaagatcgg gaaggaacaa gaacttttct ttttccacga tttgagtcct
     2641 ggaagctgtt ttttccttcc cagaggagcc ttcatttata atacgcttac agatttcata
     2701 cgagaggaat atcacaaacg ggacttcacg gaggtgctct ctcccaatat gtacaacagt
     2761 aaactctggg aagcctcagg ccactggcag cattacagcg agaacatgtt tacctttgag
     2821 attgaaaagg acacttttgc cctcaaaccc atgaattgtc cagggcactg tctaatgttt
     2881 gcccatcgtc cacgatcttg gagggaaatg cctattagat ttgctgattt tggagttctg
     2941 catagaaatg aactgtcggg gactctcagc ggcttgacca gagtgaggcg cttccagcag
     3001 gacgatgctc acattttttg cacagtggag cagattgaag aagaaataaa ggggtgtttg
     3061 cagtttttgc aatctgttta ctcaacattt ggcttctcct ttcaattaaa cctgtcaaca
     3121 aggccggaaa acttcctagg agagattgag atgtggaatg aggctgagaa gcaactgcag
     3181 aacagcttga tggactttgg agaaccgtgg aaaatgaacc caggagatgg agcattttat
     3241 ggccctaaaa ttgacataaa aatcaaggat gctattggca gataccatca atgtgctaca
     3301 attcagctgg acttccaact gcctattaga tttaatctca catatgttag taaggatggg
     3361 gatgataaga agagacctgt gatcattcat cgagccattt tgggatcagt ggaaagaatg
     3421 atagccattc tttcagaaaa ctatggcgga aaatggcctt tctggctatc tcctcgtcag
     3481 gtgatggtca tccctgtggg gccaacttgt gaaaaatatg cacttcaggt atccagtgaa
     3541 ttttttgaag aaggatttat ggctgacgtt gacttggatc acagttgtac actaaataag
     3601 aaaatacgaa atgcacagct ggctcagtat aattttattt tggtggttgg agaaaaggaa
     3661 aagatagata atgctgtaaa cgtgcgaaca agagacaaca aaattcatgg agagatttta
     3721 gtaacttctg ccattgataa actgaagaat ctcaggaaga cacggacact caatgctgag
     3781 gaggcctttt gaggatccac cggatctaga taactgatca taatcagcca taccacattt
     3841 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     3901 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     3961 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     4021 tccaaactca tcaatgtatc ttaacgcgta aattgtaagc gttaatattt tgttaaaatt
     4081 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
     4141 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
     4201 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     4261 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
     4321 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     4381 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
     4441 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     4501 cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     4561 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     4621 ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg
     4681 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     4741 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     4801 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     4861 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     4921 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     4981 ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca tgattgaaca
     5041 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     5101 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     5161 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc
     5221 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     5281 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     5341 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     5401 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     5461 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     5521 gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct
     5581 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     5641 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     5701 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     5761 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     5821 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga
     5881 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     5941 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccctagg
     6001 gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca
     6061 ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg
     6121 tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc
     6181 gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc
     6241 aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact ttagattgat
     6301 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     6361 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     6421 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     6481 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     6541 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     6601 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     6661 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     6721 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     6781 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     6841 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     6901 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     6961 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     7021 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     7081 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat gcat
//
