LOCUS V023328 6860 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V023328 VERSION V023328 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 61..364 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 365..568 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 624..2759 /label="TAB3(NM_152787)" /note="TAB3(NM_152787)" /gene="TAB3" CDS 2817..3527 /codon_start=1 /note="mammalian codon-optimized" /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /transl_table=1 /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK*" /label="mCherry" polyA_signal 3648..3769 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(3776..4231) /direction=LEFT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 4258..4362 /gene="bla" /label="AmpR promoter" promoter 4364..4721 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4756..5550 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5782..5829 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 6158..6746 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgagcc accatggcgc aaagcagccc acagcttgat attcaggttc 661 tccatgatct tcgacaacgt ttccctgaaa ttccagaggg cgtggtgtct cagtgcatgt 721 tacagaataa caacaatctt gaagcctgtt gccgagccct ttcccaggag agtagcaaat 781 acttatatat ggaataccat agtccagatg acaataggat gaatagaaat cgccttttac 841 atattaacct gggtatccat tctcctagta gctatcaccc aggagatgga gcccaactta 901 atggtggtcg aacactggta catagctcaa gtgatggaca tattgatcct cagcatgcag 961 caggtaaaca gctgatatgt ttagttcaag aaccacactc agctccagct gttgttgctg 1021 ctactcccaa ctacaatcca ttttttatga acgaacagaa cagaagtgca gctactcctc 1081 cttcacaacc acctcaacag ccatcttcca tgcaaacagg aatgaatccg tctgctatgc 1141 aagggccttc accaccaccg ccacctcctt catacatgca catacctcgg tatagtacaa 1201 atccaattac tgttacagta tcccagaacc tcccttctgg acagactgta ccaagagctt 1261 tacaaattct tccacaaatt ccaagcaatc tctatgggtc tcctggttct atttatatta 1321 gacagacatc tcagagttca tcaggaagac aaactcctca gagtacgccg tggcagtcct 1381 caccacaggg cccagtgcct cactatagcc agcgtccttt acctgtttat ccacaccaac 1441 agaactatca gccttctcag tattctccca aacagcagca gatccctcag tctgcttacc 1501 attcaccacc tccttctcaa tgtccttcac ccttcagctc tccacagcat caagtgcaac 1561 cttcccagtt gggccacatc tttatgccac ctagtccttc aactactcca ccccatccat 1621 atcaacaagg acctcctagc tatcagaaac agggaagcca ttcagtagcc tatcttccat 1681 acacagcatc tagtttatcc aaaggttcca tgaagaagat agaaattaca gttgaacctt 1741 ctcaaagacc tgggacagca attaatagga gtccttcacc catcagtaat caaccatctc 1801 cacggaatca acactcactg tacacagcca ccacgccacc ttcaagttct ccttcaagag 1861 ggatatctag tcaaccaaaa cctccattta gtgttaatcc tgtgtatatt acatatacac 1921 agccaactgg accttcttgt actccatcac catctcctcg agtgatacca aacccaacta 1981 cagtttttaa aattaccgta ggccgagcaa cgactgaaaa tcttttaaat ttagtggacc 2041 aagaagagcg ctctgcagca ccagaaccta ttcagcccat ttcagtgata ccaggctctg 2101 ggggagaaaa gggaagccat aaatatcaga gaagttctag ttctggatca gatgactatg 2161 cctacacaca agccttgctg ttacatcaac gagcaaggat ggagaggtta gcaaagcaac 2221 tgaaacttga gaaagaggag ctagagcggt tgaagtctga agttaatggt atggagcatg 2281 acctgatgca gagacggctc agaagagtca gctgcaccac tgcgatccct acgcctgagg 2341 aaatgacaag attgagaagc atgaacagac aactccagat aaatgttgac tgtacactga 2401 aagaagttga cctccttcaa tctagaggaa actttgatcc aaaagccatg aataattttt 2461 atgacaacat agaacctggc ccagttgtac cacccaagcc atctaaaaaa gactcctcag 2521 acccctgcac aattgagaga aaagcccgaa gaattagcgt gacctccaaa gtacaggcag 2581 acatccatga cacccaggca gcagctgcag atgagcatcg aactggctcc acacaaagtc 2641 ctcggacaca acctcgagat gaagactacg aaggggctcc atggaattgt gatagctgca 2701 cctttcttaa ccacccagca ctaaatcgct gtgagcagtg cgagatgcca cggtacacca 2761 agcttcgaat tctgcagtcg acggtaccgc gggcccggga tccaccggtc gccaccatgg 2821 tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc 2881 acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc 2941 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg 3001 cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg 3061 ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga 3121 tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg 3181 agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc 3241 agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc 3301 tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg 3361 tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca 3421 tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg 3481 ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtagcgg ccgcgactct 3541 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac 3601 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg 3661 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt 3721 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt 3781 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt 3841 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg 3901 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc 3961 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca 4021 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga 4081 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa 4141 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc 4201 gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc 4261 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa 4321 taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc 4381 agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa 4441 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc 4501 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc 4561 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct 4621 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga 4681 agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga 4741 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg 4801 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc 4861 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt 4921 gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt 4981 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc 5041 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc 5101 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac 5161 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag 5221 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag 5281 gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat 5341 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg 5401 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa 5461 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc 5521 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc 5581 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt 5641 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca 5701 tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac 5761 cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt 5821 cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga 5881 gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt 5941 cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag 6001 gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg 6061 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact 6121 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg 6181 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc 6241 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata 6301 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta 6361 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc 6421 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg 6481 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac 6541 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg 6601 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt 6661 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct 6721 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg 6781 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata 6841 accgtattac cgccatgcat //