LOCUS       V028316                 6507 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V028316
VERSION     V028316
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             613..1320
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             1354..3162
                     /label="STXBP1(NM_003165)"
                     /note="STXBP1(NM_003165)"
                     /gene="STXBP1"
     polyA_signal    3295..3416
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3423..3878)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3905..4009
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        4011..4368
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4403..5197
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5429..5476
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5805..6393
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
      661 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
      721 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
      781 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
      841 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
      901 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
      961 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     1021 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     1081 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     1141 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     1201 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     1261 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     1321 tccggactca gatctcgagg aggtggtgga tcaatggccc ccattggcct caaagctgtt
     1381 gtcggagaga agattatgca tgatgtgata aagaaggtca agaagaaggg ggaatggaag
     1441 gtgctggtgg tggatcagtt aagcatgagg atgctgtcct cctgctgcaa gatgacagac
     1501 atcatgaccg agggcataac gattgtggaa gatatcaata agcgcagaga gccgctcccc
     1561 agcctggagg ctgtgtatct catcactcca tccgagaagt ccgtccactc tctcatcagt
     1621 gactttaagg acccgccgac tgctaaatac cgggctgcac acgtcttctt cactgactct
     1681 tgtccagatg ccctgtttaa tgaactggta aaatcccgag cagccaaagt catcaaaact
     1741 ctgacggaaa tcaatattgc atttctcccg tatgaatccc aggtctattc cttggactct
     1801 gctgactctt tccaaagctt ctacagtccc cacaaggctc agatgaagaa tcctatactg
     1861 gagcgcctgg cagagcagat cgcgaccctt tgtgccaccc tgaaggagta cccggctgtg
     1921 cggtatcggg gggaatacaa ggacaatgcc ctgctggctc agctaatcca ggacaagctc
     1981 gatgcctata aagctgatga tccaacaatg ggggagggcc cagacaaggc acgctcccag
     2041 ctcctgatcc tggatcgagg ctttgacccc agctcccctg tgctccatga attgactttt
     2101 caggctatga gttatgatct gctgcctatc gaaaatgatg tatacaagta tgagaccagc
     2161 ggcatcgggg aggcacgggt gaaggaggtg ctcctggacg aggacgacga cctgtggata
     2221 gcactgcgcc acaagcacat cgcagaggtg tcccaggaag tcacccggtc tctgaaagat
     2281 ttttcttcta gcaagagaat gaatactgga gagaagacca ccatgcggga cctgtcccag
     2341 atgctgaaga agatgcctca gtaccagaaa gagctcagca agtactccac ccacctgcac
     2401 cttgctgagg actgtatgaa gcattaccaa ggcaccgtag acaaactctg ccgagtggag
     2461 caggacctgg ccatgggcac agatgctgag ggagagaaga tcaaggaccc tatgcgagcc
     2521 atcgtcccca ttctgctgga tgccaatgtc agcacttatg acaaaatccg catcatcctt
     2581 ctctacatct ttttgaagaa tggcatcacg gaggaaaacc tgaacaaact gatccagcac
     2641 gcccagatac ccccggagga tagtgagatc atcaccaaca tggctcacct cggcgtgccc
     2701 atcgtcaccg attccacgct gcgtcgccgg agcaagccgg agcggaagga acgcatcagc
     2761 gagcagacct accagctctc acggtggact ccgattatca aggacatcat ggaggacact
     2821 attgaggaca aacttgacac caaacactac ccttatatct ctacccgttc ctctgcctcc
     2881 ttcagcacca ccgccgtcag cgcccgctat gggcactggc ataagaacaa ggccccaggc
     2941 gagtaccgca gtggcccccg cctcatcatt ttcatccttg ggggtgtgag cctgaatgag
     3001 atgcgctgcg cctacgaggt gacccaggcc aacggaaagt gggaggtgct gataggttct
     3061 actcacattc ttactcccac caaatttctc atggacctga gacaccccga cttcagggag
     3121 tcctctaggg tatcttttga ggatcaggct ccaacaatgg agtgaggatc caccggatct
     3181 agataactga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     3241 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     3301 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     3361 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaacgc
     3421 gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     3481 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     3541 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     3601 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     3661 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     3721 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     3781 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     3841 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa
     3901 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     3961 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga
     4021 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     4081 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     4141 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     4201 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     4261 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     4321 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga
     4381 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
     4441 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     4501 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     4561 gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac
     4621 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     4681 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     4741 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     4801 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     4861 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     4921 gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     4981 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     5041 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     5101 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     5161 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
     5221 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
     5281 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     5341 gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag
     5401 acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt
     5461 gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc
     5521 ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc
     5581 ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata
     5641 gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     5701 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     5761 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     5821 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     5881 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     5941 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     6001 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     6061 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     6121 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     6181 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     6241 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     6301 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     6361 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     6421 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     6481 gtggataacc gtattaccgc catgcat
//
