LOCUS V019097 6278 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V019097 VERSION V019097 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 61..364 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 365..568 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 625..2151 /label="SOX9(NM_000346)" /note="SOX9(NM_000346)" /gene="SOX9" CDS 2224..2943 /codon_start=1 /note="mammalian codon-optimized" /product="the original enhanced GFP (Yang et al., 1996)" /transl_table=1 /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV LLEFVTAAGITLGMDELYK*" /label="EGFP" polyA_signal 3066..3187 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(3194..3649) /direction=LEFT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3676..3780 /gene="bla" /label="AmpR promoter" promoter 3782..4139 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4174..4968 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5200..5247 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 5576..6164 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgaggc caccatgaat ctcctggacc ccttcatgaa gatgaccgac 661 gagcaggaga agggcctgtc cggcgccccc agccccacca tgtccgagga ctccgcgggc 721 tcgccctgcc cgtcgggctc cggctcggac accgagaaca cgcggcccca ggagaacacg 781 ttccccaagg gcgagcccga tctgaagaag gagagcgagg aggacaagtt ccccgtgtgc 841 atccgcgagg cggtcagcca ggtgctcaaa ggctacgact ggacgctggt gcccatgccg 901 gtgcgcgtca acggctccag caagaacaag ccgcacgtca agcggcccat gaacgccttc 961 atggtgtggg cgcaggcggc gcgcaggaag ctcgcggacc agtacccgca cttgcacaac 1021 gccgagctca gcaagacgct gggcaagctc tggagacttc tgaacgagag cgagaagcgg 1081 cccttcgtgg aggaggcgga gcggctgcgc gtgcagcaca agaaggacca cccggattac 1141 aagtaccagc cgcggcggag gaagtcggtg aagaacgggc aggcggaggc agaggaggcc 1201 acggagcaga cgcacatctc ccccaacgcc atcttcaagg cgctgcaggc cgactcgcca 1261 cactcctcct ccggcatgag cgaggtgcac tcccccggcg agcactcggg gcaatcccag 1321 ggcccaccga ccccacccac cacccccaaa accgacgtgc agccgggcaa ggctgacctg 1381 aagcgagagg ggcgcccctt gccagagggg ggcagacagc cccctatcga cttccgcgac 1441 gtggacatcg gcgagctgag cagcgacgtc atctccaaca tcgagacctt cgatgtcaac 1501 gagtttgacc agtacctgcc gcccaacggc cacccggggg tgccggccac gcacggccag 1561 gtcacctaca cgggcagcta cggcatcagc agcaccgcgg ccaccccggc gagcgcgggc 1621 cacgtgtgga tgtccaagca gcaggcgccg ccgccacccc cgcagcagcc cccacaggcc 1681 ccgccggccc cgcaggcgcc cccgcagccg caggcggcgc ccccacagca gccggcggca 1741 cccccgcagc agccacaggc gcacacgctg accacgctga gcagcgagcc gggccagtcc 1801 cagcgaacgc acatcaagac ggagcagctg agccccagcc actacagcga gcagcagcag 1861 cactcgcccc aacagatcgc ctacagcccc ttcaacctcc cacactacag cccctcctac 1921 ccgcccatca cccgctcaca gtacgactac accgaccacc agaactccag ctcctactac 1981 agccacgcgg caggccaggg caccggcctc tactccacct tcacctacat gaaccccgct 2041 cagcgcccca tgtacacccc catcgccgac acctctgggg tcccttccat cccgcagacc 2101 cacagccccc agcactggga acaacccgtc tacacacagc tcactcgacc tggtaccggc 2161 ggcggaggga gtggaggagg gggaagcgga ggcggaggga gtcaggatcc accggtcgcc 2221 accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg 2281 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc 2341 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc 2401 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg 2461 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc 2521 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc 2581 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg 2641 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag 2701 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc 2761 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac 2821 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg 2881 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag 2941 taaagcggcc gcgactctag atcataatca gccataccac atttgtagag gttttacttg 3001 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg 3061 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 3121 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 3181 tatcttaagg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt 3241 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa 3301 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag 3361 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt 3421 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac 3481 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag 3541 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg 3601 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact 3661 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg 3721 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt 3781 cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag 3841 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg 3901 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag 3961 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc 4021 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg 4081 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag 4141 atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca 4201 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc 4261 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc 4321 aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg 4381 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg 4441 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct 4501 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct 4561 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa 4621 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa 4681 ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc 4741 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt 4801 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct 4861 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc 4921 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg 4981 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg 5041 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc 5101 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa 5161 cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata 5221 aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact 5281 ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc 5341 caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag 5401 gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt 5461 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac 5521 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag 5581 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg 5641 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca 5701 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga 5761 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca 5821 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc 5881 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca 5941 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa 6001 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc 6061 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc 6121 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg 6181 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat 6241 cccctgattc tgtggataac cgtattaccg ccatgcat //