LOCUS       V019097                 6278 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V019097
VERSION     V019097
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             625..2151
                     /label="SOX9(NM_000346)"
                     /note="SOX9(NM_000346)"
                     /gene="SOX9"
     CDS             2224..2943
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     polyA_signal    3066..3187
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3194..3649)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3676..3780
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        3782..4139
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4174..4968
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5200..5247
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5576..6164
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgaggc caccatgaat ctcctggacc ccttcatgaa gatgaccgac
      661 gagcaggaga agggcctgtc cggcgccccc agccccacca tgtccgagga ctccgcgggc
      721 tcgccctgcc cgtcgggctc cggctcggac accgagaaca cgcggcccca ggagaacacg
      781 ttccccaagg gcgagcccga tctgaagaag gagagcgagg aggacaagtt ccccgtgtgc
      841 atccgcgagg cggtcagcca ggtgctcaaa ggctacgact ggacgctggt gcccatgccg
      901 gtgcgcgtca acggctccag caagaacaag ccgcacgtca agcggcccat gaacgccttc
      961 atggtgtggg cgcaggcggc gcgcaggaag ctcgcggacc agtacccgca cttgcacaac
     1021 gccgagctca gcaagacgct gggcaagctc tggagacttc tgaacgagag cgagaagcgg
     1081 cccttcgtgg aggaggcgga gcggctgcgc gtgcagcaca agaaggacca cccggattac
     1141 aagtaccagc cgcggcggag gaagtcggtg aagaacgggc aggcggaggc agaggaggcc
     1201 acggagcaga cgcacatctc ccccaacgcc atcttcaagg cgctgcaggc cgactcgcca
     1261 cactcctcct ccggcatgag cgaggtgcac tcccccggcg agcactcggg gcaatcccag
     1321 ggcccaccga ccccacccac cacccccaaa accgacgtgc agccgggcaa ggctgacctg
     1381 aagcgagagg ggcgcccctt gccagagggg ggcagacagc cccctatcga cttccgcgac
     1441 gtggacatcg gcgagctgag cagcgacgtc atctccaaca tcgagacctt cgatgtcaac
     1501 gagtttgacc agtacctgcc gcccaacggc cacccggggg tgccggccac gcacggccag
     1561 gtcacctaca cgggcagcta cggcatcagc agcaccgcgg ccaccccggc gagcgcgggc
     1621 cacgtgtgga tgtccaagca gcaggcgccg ccgccacccc cgcagcagcc cccacaggcc
     1681 ccgccggccc cgcaggcgcc cccgcagccg caggcggcgc ccccacagca gccggcggca
     1741 cccccgcagc agccacaggc gcacacgctg accacgctga gcagcgagcc gggccagtcc
     1801 cagcgaacgc acatcaagac ggagcagctg agccccagcc actacagcga gcagcagcag
     1861 cactcgcccc aacagatcgc ctacagcccc ttcaacctcc cacactacag cccctcctac
     1921 ccgcccatca cccgctcaca gtacgactac accgaccacc agaactccag ctcctactac
     1981 agccacgcgg caggccaggg caccggcctc tactccacct tcacctacat gaaccccgct
     2041 cagcgcccca tgtacacccc catcgccgac acctctgggg tcccttccat cccgcagacc
     2101 cacagccccc agcactggga acaacccgtc tacacacagc tcactcgacc tggtaccggc
     2161 ggcggaggga gtggaggagg gggaagcgga ggcggaggga gtcaggatcc accggtcgcc
     2221 accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     2281 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     2341 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     2401 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
     2461 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     2521 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     2581 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     2641 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
     2701 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     2761 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     2821 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     2881 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     2941 taaagcggcc gcgactctag atcataatca gccataccac atttgtagag gttttacttg
     3001 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     3061 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     3121 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     3181 tatcttaagg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
     3241 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     3301 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     3361 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     3421 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     3481 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     3541 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     3601 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact
     3661 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     3721 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     3781 cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     3841 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     3901 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     3961 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     4021 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     4081 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag
     4141 atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
     4201 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     4261 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     4321 aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg
     4381 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     4441 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     4501 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     4561 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     4621 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     4681 ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc
     4741 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     4801 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     4861 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     4921 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
     4981 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
     5041 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     5101 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa
     5161 cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata
     5221 aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact
     5281 ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc
     5341 caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag
     5401 gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt
     5461 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     5521 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     5581 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     5641 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     5701 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     5761 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     5821 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     5881 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     5941 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     6001 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     6061 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     6121 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     6181 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     6241 cccctgattc tgtggataac cgtattaccg ccatgcat
//
