LOCUS       V047592                 5352 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V047592
VERSION     V047592
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             603..1301
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric blue fluorescent protein"
                     /transl_table=1
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
                     /label="TagBFP"
     CDS             1353..1985
                     /label="SNAP23(NM_003825)"
                     /note="SNAP23(NM_003825)"
                     /gene="SNAP23"
     CDS             1986..2003
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /transl_table=1
                     /translation="HHHHHH"
                     /label="6xHis"
     polyA_signal    2140..2261
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(2268..2723)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        2750..2854
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        2856..3213
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             3248..4042
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    4274..4321
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      4650..5238
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcca
      601 ccatgagcga gctgattaag gagaacatgc acatgaagct gtacatggag ggcaccgtgg
      661 acaaccatca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag ggcacccaga
      721 ccatgagaat caaggtggtc gagggcggcc ctctcccctt cgccttcgac atcctggcta
      781 ctagcttcct ctacggcagc aagaccttca tcaaccacac ccagggcatc cccgacttct
      841 tcaagcagtc cttccctgag ggcttcacat gggagagagt caccacatac gaagacgggg
      901 gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc tacaacgtca
      961 agatcagagg ggtgaacttc acatccaacg gccctgtgat gcagaagaaa acactcggct
     1021 gggaggcctt caccgagacg ctgtaccccg ctgacggcgg cctggaaggc agaaacgaca
     1081 tggccctgaa gctcgtgggc gggagccatc tgatcgcaaa catcaagacc acatatagat
     1141 ccaagaaacc cgctaagaac ctcaagatgc ctggcgtcta ctatgtggac tacagactgg
     1201 aaagaatcaa ggaggccaac aacgagacct acgtcgagca gcacgaggtg gcagtggcca
     1261 gatactgcga cctccctagc aaactggggc acaagcttaa tggcggcgga gggagtggag
     1321 gagggggaag cggaggcgga gggagtctcg agatggataa tctgtcatca gaagaaattc
     1381 aacagagagc tcaccagatt actgatgagt ctctggaaag tacgaggaga atcctgggtt
     1441 tagccattga gtctcaggat gcaggaatca agaccatcac tatgctggat gaacaaaagg
     1501 aacaactaaa ccgcatagaa gaaggcttgg accaaataaa taaggacatg agagagacag
     1561 agaagacttt aacagaactc aacaaatgct gtggcctttg tgtctgccca tgtaatagaa
     1621 caaagaactt tgagtctggc aaggcttata agacaacatg gggagatggt ggagaaaact
     1681 caccttgcaa tgtagtatct aaacagccag gcccggtgac aaatggtcag cttcagcaac
     1741 caacaacggg agcagccagt ggtggataca ttaaacgcat aactaatgat gccagagaag
     1801 atgaaatgga agagaacctg actcaagtgg gcagtatcct gggaaatcta aaagacatgg
     1861 ccctgaacat aggcaatgag attgatgctc aaaatccaca aataaaacga atcacagaca
     1921 aggctgacac caacagagat cgtattgata ttgccaatgc cagagcaaag aaactcattg
     1981 acagccacca tcaccatcac cattagggat ccaccggtgc ggccgcgact ctagatcata
     2041 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     2101 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     2161 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     2221 cattctagtt gtggtttgtc caaactcatc aatgtatctt aaggcgtaaa ttgtaagcgt
     2281 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     2341 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     2401 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     2461 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     2521 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     2581 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     2641 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     2701 taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     2761 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     2821 ataaatgctt caataatatt gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg
     2881 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     2941 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     3001 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     3061 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     3121 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     3181 ggaggctttt ttggaggcct aggcttttgc aaagatcgat caagagacag gatgaggatc
     3241 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     3301 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     3361 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     3421 tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     3481 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     3541 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     3601 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     3661 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     3721 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat
     3781 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     3841 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     3901 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     3961 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     4021 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg
     4081 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     4141 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     4201 ttcttcgccc accctagggg gaggctaact gaaacacgga aggagacaat accggaagga
     4261 acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt
     4321 cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat
     4381 tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa
     4441 ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc aggttactca
     4501 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     4561 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     4621 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     4681 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     4741 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     4801 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     4861 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     4921 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     4981 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     5041 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     5101 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     5161 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     5221 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     5281 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     5341 accgccatgc at
//
