LOCUS       V018677                 7742 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V018677
VERSION     V018677
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             625..3672
                     /label="SMG5(NM_015327)"
                     /note="SMG5(NM_015327)"
                     /gene="SMG5"
     CDS             3688..4407
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     polyA_signal    4530..4651
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(4658..5113)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5140..5244
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        5246..5603
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5638..6432
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6664..6711
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      7040..7628
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgaggc caccatgagc caaggccccc ccacagggga gagcagcgag
      661 cccgaagcaa aagtcctcca cactaagcgg ctttaccggg ctgtggtgga ggctgtgcat
      721 cgacttgacc tcatcctttg caacaaaact gcttatcaag aagtattcaa accagaaaac
      781 attagcctga ggaacaagct gcgtgagctc tgcgtcaagc ttatgttcct gcacccagtg
      841 gactatggga gaaaggctga ggagctgctg tggagaaagg tatactatga agttatccag
      901 cttatcaaga ctaacaaaaa gcacatccac agccggagca ctttggaatg tgcctacagg
      961 acgcacctgg ttgctggtat tggcttctac cagcatctcc ttctctatat ccagtcccac
     1021 taccagctgg aactgcagtg ctgcatcgac tggacccatg tcactgaccc cctcatagga
     1081 tgcaagaagc cagtgtctgc ctcagggaag gagatggatt gggcacagat ggcatgtcac
     1141 cgatgtctgg tgtatctggg ggatttgtcc cgatatcaga atgaattagc tggcgtagat
     1201 accgagctgc tagccgagag attttactac caagccctgt cagtagctcc tcagattgga
     1261 atgcccttca atcagctggg caccctggca ggcagcaagt actataatgt ggaagccatg
     1321 tattgctacc tgcgctgcat ccagtcagaa gtgtcctttg agggagccta tgggaacctc
     1381 aagcggctgt atgacaaggc agccaaaatg taccaccaac tgaagaagtg tgagactcgg
     1441 aaactgtctc ctggcaaaaa gcgatgtaaa gacattaaaa ggttgctagt gaactttatg
     1501 tatctgcaaa gcctcctaca gcccaaaagc agctccgtgg actcagagct gacctcactt
     1561 tgccagtcag tcctggagga cttcaacctc tgcctcttct acctgccctc ctcacccaac
     1621 ctcagcctgg ccagtgagga tgaggaggag tatgagagtg gatatgcttt cctcccggac
     1681 cttctcatct ttcaaatggt catcatctgc cttatgtgtg tgcacagctt ggagagagca
     1741 ggatccaagc agtacagtgc agccattgcc ttcaccctgg ccctcttttc ccacctcgtc
     1801 aatcatgtca acatacggct gcaggctgag ctggaagagg gcgagaatcc cgtcccggca
     1861 ttccagagtg atggcacaga tgaaccagag tccaaggaac ctgtggagaa agaggaggag
     1921 ccagatcctg agcctcctcc tgtaacaccc caagtgggtg agggcagaaa gagccgtaag
     1981 ttctctcgcc tctcctgtct ccgccgtcgc cgccacccac ccaaagttgg tgatgacagt
     2041 gacctgagtg aaggctttga atcggactca agccatgact cagcccgggc cagtgagggc
     2101 tcagacagtg gctctgacaa gagtcttgaa ggtgggggaa cggcctttga tgctgaaaca
     2161 gactcggaaa tgaatagcca ggagtcccga tcagacttgg aagatatgga ggaagaggag
     2221 gggacacggt caccaaccct ggagccccct cggggcagat cagaggctcc cgattccctc
     2281 aatggcccac tgggccccag tgaggctagc attgccagca atctacaagc catgtccacc
     2341 cagatgttcc agactaagcg ctgcttccga ctggccccca cctttagcaa cctgctcctc
     2401 cagcccacca ccaaccctca tacctcggcc agccacaggc cttgcgtcaa tggggatgta
     2461 gacaagcctt cagagccagc ctctgaggag ggctctgagt cggaggggag tgagtccagt
     2521 ggacgctcct gtcggaatga gcgcagcatc caggagaagc ttcaggtcct gatggccgaa
     2581 ggtctgcttc ctgctgtgaa agtcttcctg gactggcttc ggaccaaccc cgacctcatc
     2641 atcgtgtgtg cgcagagctc tcaaagtctg tggaaccgcc tgtctgtgtt gctgaatctg
     2701 ttgcctgctg ctggtgaact ccaggagtct ggcctggcct tgtgtcctga ggtccaagat
     2761 cttcttgaag gttgtgaact gcctgacctc ccctctagcc ttctgctccc agaggacatg
     2821 gctcttcgta acctgccccc gctccgagct gcccacagac gctttaactt tgacacggat
     2881 cggcccctgc tcagcacctt agaggagtca gtggtgcgca tctgctgcat ccgcagcttt
     2941 ggtcatttca tcgcccgcct gcaaggcagc atcctgcagt tcaacccaga ggttggcatc
     3001 ttcgtcagca ttgcccagtc tgagcaggag agcctgctgc agcaggccca ggcacagttc
     3061 cgaatggcac aggaggaagc tcgtcggaac aggctcatga gagacatggc tcagctacga
     3121 cttcagctcg aagtgtctca gctggagggc agcctgcagc agcccaaggc ccagtcagcc
     3181 atgtctccct acctcgtccc tgacacccag gccctctgcc accatctccc tgtcatccgc
     3241 caactggcca ccagtggccg cttcattgtc atcatcccaa ggacagtgat cgatggcctg
     3301 gatttgctga agaaggaaca cccaggggcc cgggatggga ttcggtacct ggaggcagag
     3361 tttaaaaaag gaaacaggta cattcgctgc cagaaagagg tgggaaagag ctttgagcgg
     3421 cataagctga agaggcagga tgcagatgcc tggactctct ataagatcct agacagctgc
     3481 aaacagctga ctctggccca gggggcaggt gaggaggatc cgagtggcat ggtgaccatc
     3541 atcacaggcc ttccactgga caaccccagc gtgctttcag gccccatgca ggcagccctg
     3601 caggccgctg cccacgccag tgtggacatc aagaatgttc tggacttcta caagcagtgg
     3661 aaggaaattg gttcaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg
     3721 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     3781 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     3841 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     3901 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     3961 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     4021 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     4081 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     4141 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     4201 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     4261 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
     4321 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     4381 ctcggcatgg acgagctgta caagtaaagc ggccgcgact ctagatcata atcagccata
     4441 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     4501 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     4561 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     4621 gtggtttgtc caaactcatc aatgtatctt aaggcgtaaa ttgtaagcgt taatattttg
     4681 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
     4741 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
     4801 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
     4861 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
     4921 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
     4981 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
     5041 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
     5101 ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     5161 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     5221 caataatatt gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc
     5281 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     5341 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     5401 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     5461 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     5521 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     5581 ttggaggcct aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg
     5641 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     5701 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     5761 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa
     5821 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     5881 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     5941 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     6001 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     6061 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     6121 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc
     6181 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     6241 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     6301 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     6361 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     6421 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc
     6481 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
     6541 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
     6601 accctagggg gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta
     6661 tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg
     6721 gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat
     6781 acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc
     6841 tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt
     6901 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     6961 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     7021 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     7081 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     7141 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     7201 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     7261 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     7321 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     7381 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     7441 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     7501 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     7561 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     7621 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     7681 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgccatgc
     7741 at
//
