LOCUS       V027507                 6055 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V027507
VERSION     V027507
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             613..1329
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             1384..2679
                     /label="SLC35B2(NM_178148)"
                     /note="SLC35B2(NM_178148)"
                     /gene="SLC35B2"
     polyA_signal    2843..2964
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(2971..3426)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3453..3557
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        3559..3916
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             3951..4745
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    4977..5024
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5353..5941
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgaggc ggcggaggga gtggaggagg gggaagcaag
     1381 cttatggacg ccagatggtg ggcagtggtg gtgctggctg cgttcccctc cctaggggca
     1441 ggtggggaga ctcccgaagc ccctccggag tcatggaccc agctatggtt cttccgattt
     1501 gtggtgaatg ctgctggcta tgccagcttt atggtacctg gctacctcct ggtgcagtac
     1561 ttcaggcgga agaactacct ggagaccggt aggggcctct gctttcccct ggtgaaagct
     1621 tgtgtgtttg gcaatgagcc caaggcctct gatgaggttc ccctggcgcc ccgaacagag
     1681 gcggcagaga ccaccccgat gtggcaggcc ctgaagctgc tcttctgtgc cacagggctc
     1741 caggtgtctt atctgacttg gggtgtgctg caggaaagag tgatgacccg cagctatggg
     1801 gccacagcca catcaccggg tgagcgcttt acggactcgc agttcctggt gctaatgaac
     1861 cgagtgctgg cactgattgt ggctggcctc tcctgtgttc tctgcaagca gccccggcat
     1921 ggggcaccca tgtaccggta ctcctttgcc agcctgtcca atgtgcttag cagctggtgc
     1981 caatacgaag ctcttaagtt cgtcagcttc cccacccagg tgctggccaa ggcctctaag
     2041 gtgatccctg tcatgctgat gggaaagctt gtgtctcggc gcagctacga acactgggag
     2101 tacctgacag ccaccctcat ctccattggg gtcagcatgt ttctgctatc cagcggacca
     2161 gagccccgca gctccccagc caccacactc tcaggcctca tcttactggc aggttatatt
     2221 gcttttgaca gcttcacctc aaactggcag gatgccctgt ttgcctataa gatgtcatcg
     2281 gtgcagatga tgtttggggt caatttcttc tcctgcctct tcacagtggg ctcactgcta
     2341 gaacaggggg ccctactgga gggaacccgc ttcatggggc gacacagtga gtttgctgcc
     2401 catgccctgc tactctccat ctgctccgca tgtggccagc tcttcatctt ttacaccatt
     2461 gggcagtttg gggctgccgt cttcaccatc atcatgaccc tccgccaggc ctttgccatc
     2521 cttctttcct gccttctcta tggccacact gtcactgtgg tgggagggct gggggtggct
     2581 gtggtctttg ctgccctcct gctcagagtc tacgcgcggg gccgtctaaa gcaacgggga
     2641 aagaaggctg tgcctgttga gtctcctgtg cagaaggttt gagaattctg cagtcgacgg
     2701 taccgcgggc ccgggatcca ccggatctag ataactgatc ataatcagcc ataccacatt
     2761 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
     2821 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
     2881 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     2941 gtccaaactc atcaatgtat cttaacgcgt aaattgtaag cgttaatatt ttgttaaaat
     3001 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     3061 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     3121 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     3181 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     3241 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     3301 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     3361 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     3421 gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     3481 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     3541 attgaaaaag gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg
     3601 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     3661 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     3721 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     3781 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     3841 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     3901 cctaggcttt tgcaaagatc gatcaagaga caggatgagg atcgtttcgc atgattgaac
     3961 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     4021 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     4081 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg
     4141 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     4201 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     4261 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     4321 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     4381 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     4441 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc
     4501 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     4561 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     4621 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     4681 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     4741 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     4801 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     4861 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccctag
     4921 ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc
     4981 aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt gttcataaac gcggggttcg
     5041 gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg
     5101 cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc
     5161 caacgtcggg gcggcaggcc ctgccatagc ctcaggttac tcatatatac tttagattga
     5221 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     5281 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     5341 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     5401 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     5461 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     5521 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     5581 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     5641 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     5701 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     5761 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     5821 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     5881 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     5941 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     6001 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcca tgcat
//
