LOCUS       V033740                 5827 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033740
VERSION     V033740
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             625..1728
                     /label="SH3GL1(NM_003025)"
                     /note="SH3GL1(NM_003025)"
                     /gene="SH3GL1"
     CDS             1780..2493
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of red fluorescent protein
                     from Entacmaea quadricolor&nbsp;(Merzlyak et al., 2007)"
                     /transl_table=1
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN*"
                     /label="TagRFP"
     polyA_signal    2615..2736
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(2743..3198)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3225..3329
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        3331..3688
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             3723..4517
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    4749..4796
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5125..5713
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgaggc caccatgtcg gtggcggggc tgaagaagca gttctacaag
      661 gcgagccagc tggtcagtga gaaggtcgga ggggccgagg ggaccaagct ggatgatgac
      721 ttcaaagaga tggagaagaa ggtggatgtc accagcaagg cggtgacaga agtgctggcc
      781 aggaccatcg agtacctgca gcccaaccca gcctcgcggg ctaagctgac catgctcaac
      841 acggtgtcca agatccgggg ccaggtgaag aaccccggct acccgcagtc ggaggggctt
      901 ctgggcgagt gcatgatccg ccacgggaag gagctgggcg gcgagtccaa ctttggtgac
      961 gcattgctgg atgccggcga gtccatgaag cgcctggcag aggtgaagga ctccctggac
     1021 atcgaggtca agcagaactt cattgacccc ctccagaacc tgtgcgagaa agacctgaag
     1081 gagatccagc accacctgaa gaaactggag ggccgccgcc tggactttga ctacaagaag
     1141 aagcggcagg gcaagatccc cgatgaggag ctacgccagg cgctggagaa gttcgaggag
     1201 tccaaggagg tggcagaaac cagcatgcac aacctcctgg agactgacat cgagcaggtg
     1261 agtcagctct cggccctggt ggatgcacag ctggactacc accggcaggc cgtgcagatc
     1321 ctggacgagc tggcggagaa gctcaagcgc aggatgcggg aagcttcctc acgccctaag
     1381 cgggagtata agcccaagcc ccgggagccc tttgaccttg gagagcctga gcagtccaac
     1441 gggggcttcc cctgcaccac agcccccaag atcgcagctt catcgtcttt ccgatcttcc
     1501 gacaagccca tccggacccc tagccggagc atgccgcccc tggaccagcc gagctgcaag
     1561 gcgctgtacg acttcgagcc cgagaacgac ggggagctgg gcttccatga gggcgacgtc
     1621 atcacgctga ccaaccagat cgatgagaac tggtacgagg gcatgctgga cggccagtcg
     1681 ggcttcttcc cgctcagcta cgtggaggtg cttgtgcccc tgccgcaggg tggaggcgga
     1741 tcaggaggcg gtggatcttc ggatccaccg gtcgccacca tggtgtctaa gggcgaagag
     1801 ctgattaagg agaacatgca catgaagctg tacatggagg gcaccgtgaa caaccaccac
     1861 ttcaagtgca catccgaggg cgaaggcaag ccctacgagg gcacccagac catgagaatc
     1921 aaggtggtcg agggcggccc tctccccttc gccttcgaca tcctggctac cagcttcatg
     1981 tacggcagca gaaccttcat caaccacacc cagggcatcc ccgacttctt taagcagtcc
     2041 ttccctgagg gcttcacatg ggagagagtc accacatacg aagacggggg cgtgctgacc
     2101 gctacccagg acaccagcct ccaggacggc tgcctcatct acaacgtcaa gatcagaggg
     2161 gtgaacttcc catccaacgg ccctgtgatg cagaagaaaa cactcggctg ggaggccaac
     2221 accgagatgc tgtaccccgc tgacggcggc ctggaaggca gaagcgacat ggccctgaag
     2281 ctcgtgggcg ggggccacct gatctgcaac ttcaagacca catacagatc caagaaaccc
     2341 gctaagaacc tcaagatgcc cggcgtctac tatgtggacc acagactgga aagaatcaag
     2401 gaggccgaca aagagaccta cgtcgagcag cacgaggtgg ctgtggccag atactgcgac
     2461 ctccctagca aactggggca caaacttaat tgagcggccg cgactctaga tcataatcag
     2521 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     2581 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     2641 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     2701 tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta agcgttaata
     2761 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     2821 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     2881 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     2941 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
     3001 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     3061 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     3121 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     3181 cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     3241 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     3301 tgcttcaata atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt
     3361 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     3421 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     3481 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     3541 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     3601 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     3661 cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc
     3721 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     3781 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     3841 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     3901 tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     3961 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     4021 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     4081 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     4141 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     4201 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg
     4261 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     4321 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     4381 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     4441 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     4501 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
     4561 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     4621 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     4681 cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg
     4741 cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa
     4801 acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg
     4861 ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc
     4921 agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat
     4981 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     5041 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     5101 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     5161 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     5221 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     5281 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     5341 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     5401 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     5461 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     5521 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     5581 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     5641 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     5701 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     5761 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     5821 catgcat
//
