LOCUS V030155 6183 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V030155 VERSION V030155 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 61..364 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 365..568 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 613..1320 /codon_start=1 /note="mammalian codon-optimized" /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /transl_table=1 /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" /label="mCherry" CDS 1378..2838 /label="Rnf8(NM_001025727)" /note="Rnf8(NM_001025727)" /gene="Rnf8" polyA_signal 2971..3092 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(3099..3554) /direction=LEFT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3581..3685 /gene="bla" /label="AmpR promoter" promoter 3687..4044 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4079..4873 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5105..5152 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 5481..6069 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc 661 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc 721 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt 781 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc 841 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc 901 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc 961 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc 1021 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac 1081 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc 1141 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc 1201 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg 1261 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag 1321 tccggactca gatctcgagc tggaggtgga ggtagtggag gaggcggaag caagcttatg 1381 ggcgagcccg accccttggt ctcggggcag ctcgcggccc gccggagctg gtgcttgcgg 1441 cggctgggga tggaccgcga gtggctacag ctggaggctg ggagtgaggt gaccatagga 1501 cggggattca gtgtcacgta ccagctgatc tcaaaggtgt gccctctgat gatttctcga 1561 aaccactgcg ttctgaagca gaatcctgag ggacaatgga cgattatgga caataagagt 1621 ctgaatggtg tttggctgaa cagagagcgt ctggcgccgt tacagggtta ctgcatccgt 1681 aagggagacc acatccagct cggagtgcct ctggaaagca aggagcatgc ggagtacgaa 1741 tacgaagtta tcgaagaaga ccgggagagt ctggctccct gccttgcccc caagaatgac 1801 cacacaacgg aaaaacataa aggcttgaga actaagagga agttcagttc ggatggggta 1861 gagagtcttc cagccgaggg cccctcagat ctcaggtgcc cactggctaa aggctccagt 1921 aaacccgctg agccagagaa gttacacggc aaaggtgaag cggcaagtca gcccttggga 1981 tgtttgtgcc ctaccttggc gtctcttgag gcgagtgaga ggaccgcggg gcctcatgct 2041 tgctctactc ttcctaaggt cctcgaactc tatcctaaga agcagaaggc ctgcagccca 2101 tcagcatctc agagcagctt agaactgttt aagatgacca tgtccaggat gctcaagctg 2161 aaaacccaga tgcaagaaaa gcagattgct gtcctgaacg tcaagaggca ggcccggaag 2221 gggagctcaa agaaagtcgt gaggatggag aaggagctgc gagacctaca gtcccagctg 2281 tacgcagagc aggctcagca gcaggcaaga gtggagcagc tggagaagac gttccaggag 2341 gaggagcagc acctccaggg tttggagaaa gagcaaggag agtgtgatct gaagcaacag 2401 ctgctgcagg ctctgcagga gcatcgcgct ctaatggaag aactagatcg cagcaagaag 2461 gactttgaga aaatcattca agccaagaac aaagaattgg agcggaccaa ggaagaaaag 2521 gacaaggtcc aagcacagaa ggaggaagtt ctcagccaca tgaacgacgt gctggagaac 2581 gagctccagt gcatcatctg ctcagagtac ttcatcgagg ctgtcaccct gaactgtgcc 2641 cacagtttct gctccttctg catcagtgag tggatgaagc ggaaagtgga gtgtcccatc 2701 tgtcggaagg acattgagtc cagaaccaac tcgctggttc tggacaactg catcagtaag 2761 atggtggaac gcctgagctc agacgtgaag gagaggagaa gcgtcctcat tagggaacga 2821 agagcaaaga gactgttatg aggatccacc ggatctagat aactgatcat aatcagccat 2881 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg 2941 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac 3001 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 3061 tgtggtttgt ccaaactcat caatgtatct taacgcgtaa attgtaagcg ttaatatttt 3121 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat 3181 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt 3241 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt 3301 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag 3361 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg 3421 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc 3481 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc 3541 gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt 3601 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct 3661 tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt 3721 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat 3781 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg 3841 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg 3901 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt 3961 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt 4021 tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat 4081 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg 4141 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc 4201 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca 4261 agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct 4321 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga 4381 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg 4441 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat 4501 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga 4561 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg 4621 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg 4681 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat 4741 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct 4801 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga 4861 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg 4921 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt 4981 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc 5041 caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct 5101 atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc 5161 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa 5221 tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg 5281 ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt 5341 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat 5401 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta 5461 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa 5521 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt 5581 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag 5641 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta 5701 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca 5761 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag 5821 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa 5881 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga 5941 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc 6001 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc 6061 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt 6121 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg 6181 cat //