LOCUS V029898 5383 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V029898 VERSION V029898 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 61..364 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 365..568 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 613..1323 /codon_start=1 /note="mammalian codon-optimized" /product="monomeric derivative of red fluorescent protein from Entacmaea quadricolor (Merzlyak et al., 2007)" /transl_table=1 /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV ARYCDLPSKLGHKLN" /label="TagRFP" CDS 1387..2007 /label="Rab7a(NM_023950)" /note="Rab7a(NM_023950)" /gene="Rab7a" polyA_signal 2171..2292 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(2299..2754) /direction=LEFT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2781..2885 /gene="bla" /label="AmpR promoter" promoter 2887..3244 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3279..4073 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4305..4352 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 4681..5269 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggtcgcca ccatggtgtc taagggcgaa gagctgatta aggagaacat gcacatgaag 661 ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga gggcgaaggc 721 aagccctacg agggcaccca gaccatgaga atcaaggtgg tcgagggcgg ccctctcccc 781 ttcgccttcg acatcctggc taccagcttc atgtacggca gcagaacctt catcaaccac 841 acccagggca tccccgactt ctttaagcag tccttccctg agggcttcac atgggagaga 901 gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag cctccaggac 961 ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa cggccctgtg 1021 atgcagaaga aaacactcgg ctgggaggcc aacaccgaga tgctgtaccc cgctgacggc 1081 ggcctggaag gcagaagcga catggccctg aagctcgtgg gcgggggcca cctgatctgc 1141 aacttcaaga ccacatacag atccaagaaa cccgctaaga acctcaagat gcccggcgtc 1201 tactatgtgg accacagact ggaaagaatc aaggaggccg acaaagagac ctacgtcgag 1261 cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg gcacaaactt 1321 aattccggac tcagatctcg aggcggcgga gggagtggag gagggggaag cggaggcgga 1381 gggagtatga cctctaggaa gaaagtgttg ctgaaggtca tcatcctggg agactctggt 1441 gttggaaaga catcgctcat gaaccagtat gtgaataaga agttcagtaa ccagtacaaa 1501 gccacaatag gagcagactt tctgaccaag gaggtgatgg tggatgacag acttgttacc 1561 atgcagatct gggacacagc tggtcaagaa cggttccagt ctcttggtgt ggccttctac 1621 agaggtgcag attgctgcgt tctggtgttt gatgtgactg cccccaacac attcaaaacc 1681 cttgacagct ggagagacga gtttctcatc caggccagcc cccgggatcc tgagaacttc 1741 ccttttgttg tgttgggaaa caagattgac ctggaaaaca gacaagtggc cacaaagagg 1801 gcacaggctt ggtgctacag caaaaacaac attccttact tcgagaccag tgccaaggag 1861 gccatcaatg tggaacaggc cttccagaca attgctcgga atgcccttaa acaggaaacg 1921 gaagtggagc tgtacaatga attccctgaa cccatcaaac tggacaagaa cgagcgggcc 1981 aaggcctccg cagagagctg cagctgttga gaattctgca gtcgacggta ccgcgggccc 2041 gggatccacc ggatctagat aactgatcat aatcagccat accacatttg tagaggtttt 2101 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat 2161 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 2221 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 2281 caatgtatct taacgcgtaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt 2341 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat 2401 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat 2461 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac 2521 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc 2581 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga 2641 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca 2701 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg 2761 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa 2821 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga 2881 agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc 2941 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag 3001 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta 3061 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc 3121 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc 3181 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg 3241 caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc 3301 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga 3361 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt 3421 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat 3481 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg 3541 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg 3601 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc 3661 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga 3721 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag 3781 ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc 3841 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg 3901 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata 3961 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg 4021 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac 4081 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc 4141 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat 4201 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg ggaggctaac 4261 tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca 4321 gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg 4381 gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt 4441 ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc 4501 ggcaggccct gccatagcct caggttactc atatatactt tagattgatt taaaacttca 4561 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc 4621 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc 4681 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc 4741 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt 4801 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt 4861 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc 4921 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa 4981 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac 5041 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg 5101 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga 5161 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact 5221 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa 5281 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc 5341 gttatcccct gattctgtgg ataaccgtat taccgccatg cat //