LOCUS       V036779                 6201 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V036779
VERSION     V036779
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             613..1329
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             1366..2856
                     /label="RETREG1(NM_001034850)"
                     /note="RETREG1(NM_001034850)"
                     /gene="RETREG1"
     polyA_signal    2989..3110
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3117..3572)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3599..3703
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        3705..4062
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4097..4891
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5123..5170
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5499..6087
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagg gcggcggagg gagtggagga gggggaagcc tcgagatggc gagcccggcg
     1381 cctccggagc acgccgagga gggatgcccg gctcctgccg ccgaggagca ggcgccgccg
     1441 tcgccgccac cgccccaggc atcccccgca gagcggcagc agcaggagga ggaagcgcag
     1501 gaagctgggg cggcggaggg cgcggggttg caggtggagg aggccgcggg ccgggcggcg
     1561 gccgcggtaa cctggctgct cggggagccg gtgctgtggc tgggctgccg cgccgacgag
     1621 ctgctgagct ggaagaggcc gctgcggagc ctgctcggct tcgtcgctgc caacctgctg
     1681 ttctggttcc ttgcattgac tccatggaga gtatatcacc tgatttccgt catgatactt
     1741 gggcgtgtta ttatgcaaat aataaaggat atggttttgt ctagaacaag aggtgcacag
     1801 ttgtggagaa gcctcagtga aagctgggaa gttatcaatt ccaaaccaga tgaaagaccc
     1861 aggctcagcc actgtattgc agaatcatgg atgaatttca gcatatttct tcaagaaatg
     1921 tctcttttta aacagcagag ccctggcaag ttttgtctcc tggtctgtag tgtgtgcaca
     1981 ttttttacga tcttgggaag ttacattcct ggggttatac tcagctatct actgttactg
     2041 tgtgcatttt tgtgtccatt gtttaaatgt aatgatattg gacaaaaaat ttacagcaaa
     2101 attaagtcag ttctgctgaa actggatttt ggaattggag aatatattaa tcagaagaaa
     2161 cgtgagagat ctgaagcaga caaagaaaaa agtcacaaag atgacagtga attagacttt
     2221 tcagctcttt gtcctaagat tagcctcacg gttgctgcca aagagttatc tgtgtctgac
     2281 acagacgtct cagaggtatc ctggactgat aatgggacct tcaacctttc agaaggatac
     2341 actccacaga cagacacttc tgatgatctt gaccgaccca gtgaggaagt tttctctaga
     2401 gatctttcag attttccatc tctagaaaat ggcatgggaa caaatgatga agatgaatta
     2461 agccttggtt tgcccactga gctcaagaga aagaaggaac agttggacag tggtcacaga
     2521 ccaagcaaag agacgcaatc agcagctggt ctcacccttc ctctgaacag tgaccaaacc
     2581 tttcacctga tgagcaacct ggctggggat gttatcacag ctgcagtgac tgcagctatc
     2641 aaagaccagt tagagggtgt gcagcaagca ctttctcagg ctgcccccat cccagaagag
     2701 gacacagaca ctgaagaagg tgatgacttt gaactacttg accagtcaga gctggatcaa
     2761 attgagagtg aattgggact tacacaagac caggaagcag aagcacagca aaataagaag
     2821 tcttcaggtt tcctttcaaa tctgctggga ggccattaag gatccaccgg atctagataa
     2881 ctgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     2941 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     3001 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     3061 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta acgcgtaaat
     3121 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     3181 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     3241 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     3301 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     3361 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     3421 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     3481 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     3541 cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg
     3601 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     3661 ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg cggaaagaac
     3721 cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     3781 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     3841 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     3901 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     3961 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     4021 aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg
     4081 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     4141 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     4201 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     4261 tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     4321 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     4381 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     4441 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     4501 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     4561 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     4621 ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     4681 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     4741 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     4801 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     4861 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
     4921 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
     4981 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     5041 atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata
     5101 ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg
     5161 tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg
     5221 agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt
     5281 tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca
     5341 ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     5401 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     5461 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     5521 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     5581 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     5641 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     5701 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     5761 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     5821 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     5881 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     5941 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     6001 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     6061 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     6121 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     6181 aaccgtatta ccgccatgca t
//
