LOCUS V022589 6986 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V022589 VERSION V022589 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 61..364 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 365..568 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 613..1329 /codon_start=1 /note="mammalian codon-optimized" /product="enhanced YFP" /transl_table=1 /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMV LLEFVTAAGITLGMDELYK" /label="EYFP" CDS 1360..3627 /label="Plcd1(NM_019676)" /note="Plcd1(NM_019676)" /gene="Plcd1" polyA_signal 3774..3895 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(3902..4357) /direction=LEFT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 4384..4488 /gene="bla" /label="AmpR promoter" promoter 4490..4847 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4882..5676 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5908..5955 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 6284..6872 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg 661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc 721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg 781 ccctggccca ccctcgtgac caccttcggc tacggcctgc agtgcttcgc ccgctacccc 841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag 901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag 961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac 1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac 1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc 1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg 1201 cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc 1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag 1321 ctgtacaagt ccggactcag atctcgagct caagcttcga tggactccgg tcgggacttc 1381 ctgaccttgc acgggctgca ggatgacccg gaccttcagg ccctcctgaa gggcagccag 1441 cttctgaagg tgaagtccag ctcgtggcgt agagaacgct tctacaagct acaggaggac 1501 tgcaagacca tctggcagga atctcgcaag gtcatgaggt ccccggagtc gcagctgttc 1561 tccatcgagg acattcagga ggtgcggatg ggacaccgca cagaaggcct ggagaaattt 1621 gccagagaca tacccgagga ccgatgcttc tccatcgtct tcaaggacca gcgcaatacc 1681 ctagacctca tcgccccgtc tccagctgac gtccagcatt gggtgcaggg cctgcgcaag 1741 atcatcgacc gctcgggctc catggaccag cggcagaagc tgcagcactg gattcactcc 1801 tgcttgcgaa aggctgataa aaacaaggat aacaagatga acttcaagga ggtgaaggac 1861 ttcctgaagg agctcaacgt acaggtggat gacagctacg ccaggaagat cttcagggaa 1921 tgtgaccact cccagacaga ctcgctggag gatgaggaga ttgagacctt ctacaggatg 1981 ctgacccagc gggcagagat cgaccgtgcc tttgcagagg ccgcaggttc tgcggagacc 2041 ctgtcggtgg agaagctagt gacgttcctg cagcatcagc agcgggagga ggaggcgggg 2101 ccagccttgg ccctctctct cattgagcgc tacgagccca gtgagactgc caaagcccag 2161 cggcagatga ccaaggatgg cttcctcatg tacctgctgt ctgctgacgg taacgccttc 2221 agcctggcgc accgccgtgt ctaccaggac atgaaccagc cgctcagcca ctacttagtg 2281 tcttcttccc acaacaccta cctactggag gaccagctca cagggcccag cagcacggag 2341 gcctacatcc gggctctgtg caaaggctgc cggtgtctgg agctggactg ctgggacggc 2401 cccaaccagg aacccatcat ctaccatggc tacactttta cctctaagat acttttctgc 2461 gacgtgctca gggccatccg ggactacgcc ttcaaggcat ccccctaccc agttatcctg 2521 tccctggaga accactgcag cctggagcaa caacgagtca tggctcatca cctgagggcc 2581 atcctggggc ccatgctgtt ggaccagccg ctggatgggg ttaccacgag cctgccttcg 2641 cctgagcaac tgaaggaaaa gattctgttg aaagggaaga aactgggcgg gctgctacct 2701 gctggagggg aaaacgggcc tgaagccacc gatgtgtctg acgaagatga ggctgctgaa 2761 atggaagatg aggccgtgcg cagccaagtg caacacaagc ccaaggagga taagctaaag 2821 ctggtgccgg agctctccga catggtcatt tactgcaaga gtgtccactt tggtggcttc 2881 tccagtccta gcacctctgg gcaggcattc tatgagatgg cttccttctc cgagagccgt 2941 gccctgcggc tgctgcaaga atcaggaaat agttttgtcc gccataacgt gggccatctg 3001 agcaggatct acccagctgg gtggaggaca gattcctcca actacagtcc cgtggagatg 3061 tggaatggag gctgccagat tgtggctctg aacttccaaa cccctgggcc agaaatggat 3121 gtttaccttg gctgcttcca ggacaatgga ggttgtgggt atgtgctgaa accggccttc 3181 ctgagagacc ctgacaccac cttcaactca cgggccctga ctcaggggcc ctggtgggcc 3241 ccgaagaagc tccgagtctg gatcatctct ggacagcagc tgccgaaggt caataagaat 3301 aagaattcta tcgtggaccc caaggtgata gtggagatcc acggtgtggg ccaggatgtg 3361 gccagccgtc agacagcagt tattaccaat aatggtttca acccacggtg ggacacggag 3421 tttgagtttg tggtagcagt gcctgacctt gcccttgtac gtttcatggt agaggattat 3481 gactcctcct ctaagaacga ctttatcggc cagagtacta tcccctggaa cagcctcaag 3541 caggggtacc gccatgtcca cctcttgtct aagaatggag acctgcatcc atcagccact 3601 ctctttgtga agatctccat ccaggactag gtaccgcggg cccgggatcc accggatcta 3661 gataactgat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc 3721 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt 3781 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag 3841 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttaaggcg 3901 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca 3961 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag 4021 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc 4081 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc 4141 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc 4201 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa 4261 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc 4321 acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat 4381 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg 4441 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa 4501 agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag 4561 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag 4621 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc 4681 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc 4741 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat 4801 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat cgatcaagag 4861 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc 4921 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat 4981 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg 5041 tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg 5101 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta 5161 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta 5221 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc 5281 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc 5341 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg 5401 ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg 5461 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt 5521 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc 5581 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc 5641 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga 5701 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg 5761 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg 5821 atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca cggaaggaga 5881 caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacggtg 5941 ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc 6001 caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc 6061 caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag 6121 cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga 6181 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt 6241 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc 6301 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc 6361 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac 6421 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac 6481 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt 6541 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct 6601 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat 6661 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt 6721 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg 6781 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt 6841 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt 6901 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg 6961 tggataaccg tattaccgcc atgcat //