LOCUS       V040802                10388 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V040802
VERSION     V040802
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             601..6321
                     /label="PTPRF(NM_002840)"
                     /note="PTPRF(NM_002840)"
                     /gene="PTPRF"
     CDS             6337..7047
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /transl_table=1
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK*"
                     /label="mNeonGreen"
     polyA_signal    7176..7297
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(7304..7759)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        7786..7890
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        7892..8249
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             8284..9078
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    9310..9357
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      9686..10274
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagccacc
      601 atggcccctg agccagcccc agggaggacg atggtgcccc ttgtgcctgc actggtgatg
      661 cttggtttgg tggcaggcgc ccatggtgac agcaaacctg tcttcattaa agtccctgag
      721 gaccagactg ggctgtcagg aggggtagcc tccttcgtgt gccaagctac aggagaaccc
      781 aagccgcgca tcacatggat gaagaagggg aagaaagtca gctcccagcg cttcgaggtc
      841 attgagtttg atgatggggc agggtcagtg cttcggatcc agccattgcg ggtgcagcga
      901 gatgaagcca tctatgagtg tacagctact aacagcctgg gtgagatcaa cactagtgcc
      961 aagctctcag tgctcgaaga ggaacagctg ccccctgggt tcccttccat cgacatgggg
     1021 cctcagctga aggtggtgga gaaggcacgc acagccacca tgctatgtgc cgcaggcgga
     1081 aatccagacc ctgagatttc ttggttcaag gacttccttc ctgtagaccc tgccacgagc
     1141 aacggccgca tcaagcagct gcgttcaggt gccttgcaga tagagagcag tgaggaatcc
     1201 gaccaaggca agtacgagtg tgtggcgacc aactcggcag gcacacgtta ctcagcccct
     1261 gcgaacctgt atgtgcgagt gcgccgcgtg gctcctcgtt tctccatccc tcccagcagc
     1321 caggaggtga tgccaggcgg cagcgtgaac ctgacatgcg tggcagtggg tgcacccatg
     1381 ccctacgtga agtggatgat gggggccgag gagctcacca aggaggatga gatgccagtt
     1441 ggccgcaacg tcctggagct cagcaatgtc gtacgctctg ccaactacac ctgtgtggcc
     1501 atctcctcgc tgggcatgat cgaggccaca gcccaggtca cagtgaaagc tcttccaaag
     1561 cctccgattg atcttgtggt gacagagaca actgccacca gtgtcaccct cacctgggac
     1621 tctgggaact cggagcctgt aacctactat ggcatccagt accgcgcagc gggcacggag
     1681 ggcccctttc aggaggtgga tggtgtggcc accacccgct acagcattgg cggcctcagc
     1741 cctttctcgg aatatgcctt ccgcgtgctg gcggtgaaca gcatcgggcg agggccgccc
     1801 agcgaggcag tgcgggcacg cacgggagaa caggcgccct ccagcccacc gcgccgcgtg
     1861 caggcacgca tgctgagcgc cagcaccatg ctggtgcagt gggagcctcc cgaggagccc
     1921 aacggcctgg tgcggggata ccgcgtctac tatactccgg actcccgccg ccccccgaac
     1981 gcctggcaca agcacaacac cgacgcgggg ctcctcacga ccgtgggcag cctgctgcct
     2041 ggcatcacct acagcctgcg cgtgcttgcc ttcaccgccg tgggcgatgg ccctcccagc
     2101 cccaccatcc aggtcaagac gcagcaggga gtgcctgccc agcccgcgga cttccaggcc
     2161 gaggtggagt cggacaccag gatccagctc tcgtggctgc tgccccctca ggagcggatc
     2221 atcatgtatg aactggtgta ctgggcggca gaggacgaag accaacagca caaggtgacc
     2281 ttcgacccaa cctcctccta cacactagag gacctgaagc ctgacacact ctaccgcttc
     2341 cagctggctg cacgctcgga tatgggggtg ggcgtcttca cccccaccat tgaggcccgc
     2401 acagcccagt ccaccccctc cgcccctccc cagaaggtga tgtgtgtgag catgggctcc
     2461 accacggtcc gggtaagttg ggtcccgccg cctgccgaca gccgcaacgg cgttatcacc
     2521 cagtactccg tggcctacga ggcggtggac ggcgaggacc gcgggcggca tgtggtggat
     2581 ggcatcagcc gtgagcactc cagctgggac ctggtgggcc tggagaagtg gacggagtac
     2641 cgggtgtggg tgcgggcaca cacagacgtg ggccccggcc ccgagagcag cccggtgctg
     2701 gtgcgcaccg atgaggacgt gcccagcggg cctccgcgga aggtggaggt ggagccactg
     2761 aactccactg ctgtgcatgt ctactggaag ctgcctgtcc ccagcaagca gcatggccag
     2821 atccgcggct accaggtcac ctacgtgcgg ctggagaatg gcgagccccg tggactcccc
     2881 atcatccaag acgtcatgct agccgaggcc cagtggcggc cagaggagtc cgaggactat
     2941 gaaaccacta tcagcggcct gaccccggag accacctact ccgttactgt tgctgcctat
     3001 accaccaagg gggatggtgc ccgcagcaag cccaaaattg tcactacaac aggtgcagtc
     3061 ccaggccggc ccaccatgat gatcagcacc acggccatga acactgcgct gctccagtgg
     3121 cacccaccca aggaactgcc tggcgagctg ctgggctacc ggctgcagta ctgccgggcc
     3181 gacgaggcgc ggcccaacac catagatttc ggcaaggatg accagcactt cacagtcacc
     3241 ggcctgcaca aggggaccac ctacatcttc cggcttgctg ccaagaaccg ggctggcttg
     3301 ggtgaggagt tcgagaagga gatcaggacc cccgaggacc tgcccagcgg cttcccccaa
     3361 aacctgcatg tgacaggact gaccacgtct accacagaac tggcctggga cccgccagtg
     3421 ctggcggaga ggaacgggcg catcatcagc tacaccgtgg tgttccgaga catcaacagc
     3481 caacaggagc tgcagaacat cacgacagac acccgcttta cccttactgg cctcaagcca
     3541 gacaccactt acgacatcaa ggtccgcgca tggaccagca aaggctctgg cccactcagc
     3601 cccagcatcc agtcccggac catgccggtg gagcaagtgt ttgccaagaa cttccgggtg
     3661 gcggctgcaa tgaagacgtc tgtgctgctc agctgggagg ttcccgactc ctataagtca
     3721 gctgtgccct ttaagattct gtacaatggg cagagtgtgg aggtggacgg gcactcgatg
     3781 cggaagctga tcgcagacct gcagcccaac acagagtact cgtttgtgct gatgaaccgt
     3841 ggcagcagcg cagggggcct gcagcacctg gtgtccatcc gcacagcccc cgacctcctg
     3901 cctcacaagc cgctgcctgc ctctgcctac atagaggacg gccgcttcga tctctccatg
     3961 ccccatgtgc aagacccctc gcttgtcagg tggttctaca ttgttgtggt gcccattgac
     4021 cgtgtgggcg ggagcatgct gacgccaagg tggagcacac ccgaggaact ggagctggac
     4081 gagcttctag aagccatcga gcaaggcgga gaggagcagc ggcggcggcg gcggcaggca
     4141 gaacgtctga agccatatgt ggctgctcaa ctggatgtgc tcccggagac ctttaccttg
     4201 ggggacaaga agaactaccg gggcttctac aaccggcccc tgtctccgga cttgagctac
     4261 cagtgctttg tgcttgcctc cttgaaggaa cccatggacc agaagcgcta tgcctccagc
     4321 ccctactcgg atgagatcgt ggtccaggtg acaccagccc agcagcagga ggagccggag
     4381 atgctgtggg tgacgggtcc cgtgctggca gtcatcctca tcatcctcat tgtcatcgcc
     4441 atcctcttgt tcaaaaggaa aaggacccac tctccgtcct ctaaggatga gcagtcgatc
     4501 ggactgaagg actccttgct ggcccactcc tctgaccctg tggagatgcg gaggctcaac
     4561 taccagaccc caggtatgcg agaccaccca cccatcccca tcaccgacct ggcggacaac
     4621 atcgagcgcc tcaaagccaa cgatggcctc aagttctccc aggagtatga gtccatcgac
     4681 cctggacagc agttcacgtg ggagaattca aacctggagg tgaacaagcc caagaaccgc
     4741 tatgcgaatg tcatcgccta cgaccactct cgagtcatcc ttacctctat cgatggcgtc
     4801 cccgggagtg actacatcaa tgccaactac atcgatggct accgcaagca gaatgcctac
     4861 atcgccacgc agggccccct gcccgagacc atgggtgatt tctggaggat ggtgtgggaa
     4921 cagcgcacgg ccactgtggt catgatgaca cggctggagg agaagtcccg ggtaaaatgt
     4981 gatcagtact ggccagcccg tggcaccgag acctgtggcc ttattcaggt gaccctgttg
     5041 gacacagtgg agctggccac atacactgtg cgcaccttcg cactccacaa gagtggctcc
     5101 agtgagaagc gcgagctgcg tcagtttcag ttcatggcct ggccagacca tggagttcct
     5161 gagtacccaa ctcccatcct ggccttccta cgacgggtca aggcctgcaa ccccctagac
     5221 gcagggccca tggtggtgca ctgcagcgcg ggcgtgggcc gcaccggctg cttcatcgtg
     5281 attgatgcca tgttggagcg gatgaagcac gagaagacgg tggacatcta tggccacgtg
     5341 acctgcatgc gatcacagag gaactacatg gtgcagacgg aggaccagta cgtgttcatc
     5401 catgaggcgc tgctggaggc tgccacgtgc ggccacacag aggtgcctgc ccgcaacctg
     5461 tatgcccaca tccagaagct gggccaagtg cctccagggg agagtgtgac cgccatggag
     5521 ctcgagttca agttgctggc cagctccaag gcccacacgt cccgcttcat cagcgccaac
     5581 ctgccctgca acaagttcaa gaaccggctg gtgaacatca tgccctacga attgacccgt
     5641 gtgtgtctgc agcccatccg tggtgtggag ggctctgact acatcaatgc cagcttcctg
     5701 gatggttata gacagcagaa ggcctacata gctacacagg ggcctctggc agagagcacc
     5761 gaggacttct ggcgcatgct atgggagcac aattccacca tcatcgtcat gctgaccaag
     5821 cttcgggaga tgggcaggga gaaatgccac cagtactggc cagcagagcg ctctgctcgc
     5881 taccagtact ttgttgttga cccgatggct gagtacaaca tgccccagta tatcctgcgt
     5941 gagttcaagg tcacggatgc ccgggatggg cagtcaagga caatccggca gttccagttc
     6001 acagactggc cagagcaggg cgtgcccaag acaggcgagg gattcattga cttcatcggg
     6061 caggtgcata agaccaagga gcagtttgga caggatgggc ctatcacggt gcactgcagt
     6121 gctggcgtgg gccgcaccgg ggtgttcatc actctgagca tcgtcctgga gcgcatgcgc
     6181 tacgagggcg tggtcgacat gtttcagacc gtgaagaccc tgcgtacaca gcgtcctgcc
     6241 atggtgcaga cagaggacca gtatcagctg tgctaccgtg cggccctgga gtacctcggc
     6301 agctttgacc actatgcaac gggaggaggg ggaagcatgg tgagcaaggg cgaggaggat
     6361 aacatggcct ctctcccagc gacacatgag ttacacatct ttggctccat caacggtgtg
     6421 gactttgaca tggtgggtca gggcaccggc aatccaaatg atggttatga ggagttaaac
     6481 ctgaagtcca ccaagggtga cctccagttc tccccctgga ttctggtccc tcatatcggg
     6541 tatggcttcc atcagtacct gccctaccct gacgggatgt cgcctttcca ggccgccatg
     6601 gtagatggct ccggatacca agtccatcgc acaatgcagt ttgaagatgg tgcctccctt
     6661 actgttaact accgctacac ctacgaggga agccacatca aaggagaggc ccaggtgaag
     6721 gggactggtt tccctgctga cggtcctgtg atgaccaact cgctgaccgc tgcggactgg
     6781 tgcaggtcga agaagactta ccccaacgac aaaaccatca tcagtacctt taagtggagt
     6841 tacaccactg gaaatggcaa gcgctaccgg agcactgcgc ggaccaccta cacctttgcc
     6901 aagccaatgg cggctaacta tctgaagaac cagccgatgt acgtgttccg taagacggag
     6961 ctcaagcact ccaagaccga gctcaacttc aaggagtggc aaaaggcctt taccgatgtg
     7021 atgggcatgg acgagctgta caagtaggat ccaccggatc tagataactg atcataatca
     7081 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     7141 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     7201 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     7261 ctagttgtgg tttgtccaaa ctcatcaatg tatcttaacg cgtaaattgt aagcgttaat
     7321 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
     7381 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
     7441 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
     7501 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
     7561 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
     7621 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
     7681 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
     7741 gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     7801 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     7861 atgcttcaat aatattgaaa aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg
     7921 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     7981 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     8041 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     8101 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     8161 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     8221 gcttttttgg aggcctaggc ttttgcaaag atcgatcaag agacaggatg aggatcgttt
     8281 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
     8341 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     8401 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     8461 ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     8521 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     8581 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     8641 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     8701 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     8761 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc
     8821 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
     8881 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     8941 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     9001 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     9061 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca
     9121 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     9181 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     9241 tcgcccaccc tagggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc
     9301 gcgctatgac ggcaataaaa agacagaata aaacgcacgg tgttgggtcg tttgttcata
     9361 aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg
     9421 gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc
     9481 cagggctcgc agccaacgtc ggggcggcag gccctgccat agcctcaggt tactcatata
     9541 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     9601 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     9661 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     9721 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     9781 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     9841 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     9901 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     9961 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
    10021 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
    10081 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
    10141 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
    10201 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
    10261 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
    10321 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
    10381 ccatgcat
//
