LOCUS       V022984                 6605 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V022984
VERSION     V022984
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             613..1329
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="enhanced CFP"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNG
                     IKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="ECFP"
     CDS             1351..3249
                     /label="PML(NM_002675)"
                     /note="PML(NM_002675)"
                     /gene="PML"
     polyA_signal    3393..3514
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3521..3976)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4003..4107
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        4109..4466
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4501..5295
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5527..5574
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5903..6491
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tggggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac atcagccaca acgtctatat caccgccgac
     1081 aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgagct atggagcctg cacccgcccg atctccgagg
     1381 ccccagcagg accccgcccg gccccaggag cccaccatgc ctccccccga gaccccctct
     1441 gaaggccgcc agcccagccc cagccccagc cctacagagc gagcccccgc ttcggaggag
     1501 gagttccagt ttctgcgctg ccagcaatgc caggcggaag ccaagtgccc gaagctgctg
     1561 ccttgtctgc acacgctgtg ctcaggatgc ctggaggcgt cgggcatgca gtgccccatc
     1621 tgccaggcgc cctggcccct aggtgcagac acacccgccc tggataacgt ctttttcgag
     1681 agtctgcagc ggcgcctgtc ggtgtaccgg cagattgtgg atgcgcaggc tgtgtgcacc
     1741 cgctgcaaag agtcggccga cttctggtgc tttgagtgcg agcagctcct ctgcgccaag
     1801 tgcttcgagg cacaccagtg gttcctcaag cacgaggccc ggcccctagc agagctgcgc
     1861 aaccagtcgg tgcgtgagtt cctggacggc acccgcaaga ccaacaacat cttctgctcc
     1921 aaccccaacc accgcacccc tacgctgacc agcatctact gccgaggatg ttccaagccg
     1981 ctgtgctgct cgtgcgcgct ccttgacagc agccacagtg agctcaagtg cgacatcagc
     2041 gcagagatcc agcagcgaca ggaggagctg gacgccatga cgcaggcgct gcaggagcag
     2101 gatagtgcct ttggcgcggt tcacgcgcag atgcacgcgg ccgtcggcca gctgggccgc
     2161 gcgcgtgccg agaccgagga gctgatccgc gagcgcgtgc gccaggtggt agctcacgtg
     2221 cgggctcagg agcgcgagct gctggaggct gtggacgcgc ggtaccagcg cgactacgag
     2281 gagatggcca gtcggctggg ccgcctggat gctgtgctgc agcgcatccg cacgggcagc
     2341 gcgctggtgc agaggatgaa gtgctacgcc tcggaccagg aggtgctgga catgcacggt
     2401 ttcctgcgcc aggcgctctg ccgcctgcgc caggaggagc cccagagcct gcaagctgcc
     2461 gtgcgcaccg atggcttcga cgagttcaag gtgcgcctgc aggacctcag ctcttgcatc
     2521 acccagggga aagatgcagc tgtatccaag aaagccagcc cagaggctgc cagcactccc
     2581 agggacccta ttgacgttga cctgcccgag gaggcagaga gagtgaaggc ccaggttcag
     2641 gccctggggc tggctgaagc ccagcctatg gctgtggtac agtcagtgcc cggggcacac
     2701 cccgtgccag tgtacgcctt ctccatcaaa ggcccttcct atggagagga tgtctccaat
     2761 acaacgacag cccagaagag gaagtgcagc cagacccagt gccccaggaa ggtcatcaag
     2821 atggagtctg aggaggggaa ggaggcaagg ttggctcgga gctccccgga gcagcccagg
     2881 cccagcacct ccaaggcagt ctcaccaccc cacctggatg gaccgcctag ccccaggagc
     2941 cccgtcatag gaagtgaggt cttcctgccc aacagcaacc acgtggccag tggcgccggg
     3001 gaggcagagg aacgcgttgt ggtgatcagc agctcggaag actcagatgc cgaaaactcg
     3061 tcctcccgag agctggatga cagcagcagt gagtccagtg acctccagct ggaaggcccc
     3121 agcaccctca gggtcctgga cgagaacctt gctgaccccc aagcagaaga cagacctctg
     3181 gttttctttg acctcaagat tgacaatgaa agtgggttct cctggggcta cccccacccc
     3241 tttctaattt agccgcgggc ccgggatcca ccggatctag ataactgatc ataatcagcc
     3301 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     3361 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     3421 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     3481 gttgtggttt gtccaaactc atcaatgtat cttaaggcgt aaattgtaag cgttaatatt
     3541 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     3601 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     3661 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     3721 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     3781 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     3841 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     3901 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     3961 ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     4021 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     4081 cttcaataat attgaaaaag gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt
     4141 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     4201 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     4261 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     4321 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     4381 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     4441 tttttggagg cctaggcttt tgcaaagatc gatcaagaga caggatgagg atcgtttcgc
     4501 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     4561 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     4621 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     4681 caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     4741 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     4801 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     4861 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     4921 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     4981 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac
     5041 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     5101 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     5161 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     5221 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     5281 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc
     5341 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     5401 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     5461 cccaccctag ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg
     5521 ctatgacggc aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt gttcataaac
     5581 gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc
     5641 aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag
     5701 ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc ctcaggttac tcatatatac
     5761 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     5821 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     5881 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     5941 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     6001 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     6061 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     6121 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     6181 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     6241 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     6301 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     6361 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     6421 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     6481 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     6541 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcca
     6601 tgcat
//
