LOCUS       V044377                 6897 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V044377
VERSION     V044377
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             613..1329
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="enhanced CFP"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNG
                     IKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="ECFP"
     CDS             1381..3552
                     /label="PIK3R1(NM_181523)"
                     /note="PIK3R1(NM_181523)"
                     /gene="PIK3R1"
     polyA_signal    3685..3806
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3813..4268)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4295..4399
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        4401..4758
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4793..5587
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5819..5866
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      6195..6783
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tggggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac atcagccaca acgtctatat caccgccgac
     1081 aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgaggc ggtggaggcg gatcaggagg cggtggatct
     1381 atgagtgctg aggggtacca gtacagagcg ctgtatgatt ataaaaagga aagagaagaa
     1441 gatattgact tgcacttggg tgacatattg actgtgaata aagggtcctt agtagctctt
     1501 ggattcagtg atggacagga agccaggcct gaagaaattg gctggttaaa tggctataat
     1561 gaaaccacag gggaaagggg ggactttccg ggaacttacg tagaatatat tggaaggaaa
     1621 aaaatctcgc ctcccacacc aaagccccgg ccacctcggc ctcttcctgt tgcaccaggt
     1681 tcttcgaaaa ctgaagcaga tgttgaacaa caagctttga ctctcccgga tcttgcagag
     1741 cagtttgccc ctcctgacat tgccccgcct cttcttatca agctcgtgga agccattgaa
     1801 aagaaaggtc tggaatgttc aactctatac agaacacaga gctccagcaa cctggcagaa
     1861 ttacgacagc ttcttgattg tgatacaccc tccgtggact tggaaatgat cgatgtgcac
     1921 gttttggctg acgctttcaa acgctatctc ctggacttac caaatcctgt cattccagca
     1981 gccgtttaca gtgaaatgat ttctttagct ccagaagtac aaagctccga agaatatatt
     2041 cagctattga agaagcttat taggtcgcct agcatacctc atcagtattg gcttacgctt
     2101 cagtatttgt taaaacattt cttcaagctc tctcaaacct ccagcaaaaa tctgttgaat
     2161 gcaagagtac tctctgaaat tttcagccct atgcttttca gattctcagc agccagctct
     2221 gataatactg aaaacctcat aaaagttata gaaattttaa tctcaactga atggaatgaa
     2281 cgacagcctg caccagcact gcctcctaaa ccaccaaaac ctactactgt agccaacaac
     2341 ggtatgaata acaatatgtc cttacaagat gctgaatggt actggggaga tatctcgagg
     2401 gaagaagtga atgaaaaact tcgagataca gcagacggga cctttttggt acgagatgcg
     2461 tctactaaaa tgcatggtga ttatactctt acactaagga aagggggaaa taacaaatta
     2521 atcaaaatat ttcatcgaga tgggaaatat ggcttctctg acccattaac cttcagttct
     2581 gtggttgaat taataaacca ctaccggaat gaatctctag ctcagtataa tcccaaattg
     2641 gatgtgaaat tactttatcc agtatccaaa taccaacagg atcaagttgt caaagaagat
     2701 aatattgaag ctgtagggaa aaaattacat gaatataaca ctcagtttca agaaaaaagt
     2761 cgagaatatg atagattata tgaagaatat acccgcacat cccaggaaat ccaaatgaaa
     2821 aggacagcta ttgaagcatt taatgaaacc ataaaaatat ttgaagaaca gtgccagacc
     2881 caagagcggt acagcaaaga atacatagaa aagtttaaac gtgaaggcaa tgagaaagaa
     2941 atacaaagga ttatgcataa ttatgataag ttgaagtctc gaatcagtga aattattgac
     3001 agtagaagaa gattggaaga agacttgaag aagcaggcag ctgagtatcg agaaattgac
     3061 aaacgtatga acagcattaa accagacctt atccagctga gaaagacgag agaccaatac
     3121 ttgatgtggt tgactcaaaa aggtgttcgg caaaagaagt tgaacgagtg gttgggcaat
     3181 gaaaacactg aagaccaata ttcactggtg gaagatgatg aagatttgcc ccatcatgat
     3241 gagaagacat ggaatgttgg aagcagcaac cgaaacaaag ctgaaaacct gttgcgaggg
     3301 aagcgagatg gcacttttct tgtccgggag agcagtaaac agggctgcta tgcctgctct
     3361 gtagtggtgg acggcgaagt aaagcattgt gtcataaaca aaacagcaac tggctatggc
     3421 tttgccgagc cctataactt gtacagctct ctgaaagaac tggtgctaca ttaccaacac
     3481 acctcccttg tgcagcacaa cgactccctc aatgtcacac tagcctaccc agtatatgca
     3541 cagcagaggc gatgaggatc caccggatct agataactga tcataatcag ccataccaca
     3601 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     3661 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     3721 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     3781 ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta agcgttaata ttttgttaaa
     3841 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     3901 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
     3961 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     4021 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
     4081 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     4141 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
     4201 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     4261 gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     4321 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     4381 atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta
     4441 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     4501 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     4561 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     4621 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     4681 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     4741 ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga
     4801 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     4861 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     4921 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga
     4981 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     5041 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     5101 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     5161 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     5221 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     5281 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga
     5341 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     5401 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     5461 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     5521 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     5581 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
     5641 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     5701 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct
     5761 agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg
     5821 gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt
     5881 cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc
     5941 cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca
     6001 gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat actttagatt
     6061 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     6121 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     6181 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     6241 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     6301 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     6361 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     6421 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     6481 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     6541 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     6601 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     6661 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     6721 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     6781 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     6841 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc catgcat
//
