LOCUS       V018535                 6948 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V018535
VERSION     V018535
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             625..2862
                     /label="NIBAN2(NM_022833)"
                     /note="NIBAN2(NM_022833)"
                     /gene="NIBAN2"
     CDS             2917..3612
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved
                     from a synthetic template (Bindels et al., 2016)"
                     /transl_table=1
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
                     /label="mScarlet"
     polyA_signal    3736..3857
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3864..4319)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4346..4450
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        4452..4809
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4844..5638
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5870..5917
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      6246..6834
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgaggc caccatgggg gacgtgctgt ccacgcacct ggacgacgcc
      661 cggcgccagc acatcgcaga aaaaaccggg aagatcctga cggagttcct ccagttctat
      721 gaagaccagt atggcgtggc tctcttcaac agcatgcgcc atgagattga gggcacgggg
      781 ctgccgcagg cccagctgct ctggcgcaag gtgccactgg acgagcgcat cgtcttctcg
      841 gggaacctct tccagcacca ggaggacagc aagaagtgga gaaaccgctt cagcctcgtg
      901 ccccacaact acgggctggt gctctacgaa aacaaagcgg cctatgagcg gcaggtccca
      961 ccacgagccg tcatcaacag tgcaggctac aaaatcctca cgtccgtgga ccaatacctg
     1021 gagctcattg gcaactcctt accagggacc acggcaaagt cgggcagtgc ccccatcctc
     1081 aagtgcccca cacagttccc gctcatcctc tggcatcctt atgcgcgtca ctactacttc
     1141 tgcatgatga cagaagccga gcaggacaag tggcaggctg tgctgcagga ctgcatccgg
     1201 cactgcaaca atggaatccc tgaggactcc aaggtagagg gccctgcgtt cacagatgcc
     1261 atccgcatgt accgacagtc caaggagctg tacggcacct gggagatgct gtgtgggaac
     1321 gaggtgcaga tcctgagcaa cctggtgatg gaggagctgg gccctgagct gaaggcagag
     1381 ctcggcccgc ggctgaaggg gaaaccgcag gagcggcagc ggcagtggat ccagatctcg
     1441 gacgccgtgt accacatggt gtacgagcag gccaaggcgc gcttcgagga ggtgctgtcc
     1501 aaggtgcagc aggtgcagcc ggccatgcag gccgtcatcc gaactgacat ggaccaaatt
     1561 atcacctcca aggagcacct tgccagcaag atccgagcct tcatcctccc caaggcagag
     1621 gtgtgcgtgc ggaaccatgt ccagccctac atcccatcca tcctggaggc cctgatggtc
     1681 cccaccagcc agggcttcac tgaggtgcga gatgtcttct tcaaggaggt cacggacatg
     1741 aacctgaacg tcatcaacga gggcggcatt gacaagctgg gcgagtacat ggagaagctg
     1801 tcccggctgg cgtaccaccc cctgaagatg cagagctgct atgagaagat ggagtcgctg
     1861 cgactggacg ggctgcagca gcgatttgat gtgtccagca cgtccgtgtt caagcagcga
     1921 gcccagatcc acatgcggga gcaaatggac aatgccgtgt atacgttcga gaccctcctg
     1981 caccaggagc tggggaaggg gcccaccaag gaggagctgt gcaagtccat ccagcgggtc
     2041 ctggagcggg tgctgaagaa atacgactac gacagcagct ctgtgcggaa gaggttcttc
     2101 cgggaggcgc tgctgcagat cagcatcccg ttcctgctca agaagctggc ccctacctgc
     2161 aagtcggagc tgccccggtt ccaggagctg atcttcgagg actttgccag gttcatcctg
     2221 gtggaaaaca cgtacgagga ggtggtgctg cagaccgtca tgaaggacat cctgcaggct
     2281 gtgaaggagg ccgcggtgca gaggaagcac aacctctacc gggacagcat ggtcatgcac
     2341 aacagcgacc ccaacctgca cctgctggcc gagggcgccc ccatcgactg gggcgaggag
     2401 tacagcaaca gcggcggggg cggcagcccc agccccagca ccccggagtc agccaccctc
     2461 tcggaaaagc gacggcgcgc caagcaggtg gtctctgtgg tccaggatga ggaggtgggg
     2521 ctgccctttg aggctagccc tgagtcacca ccacctgcgt ccccggacgg tgtcactgag
     2581 atccgaggcc tgctggccca aggtctgcgg cctgagagcc ccccaccagc cggccccctg
     2641 ctcaacgggg cccccgctgg ggagagtccc cagcctaagg ccgcccccga ggcctcctcg
     2701 ccgcctgcct cacccctcca gcatctcctg cctggaaagg ctgtggacct tgggcccccc
     2761 aagcccagcg accaggagac tggagagcag gtgtccagcc ccagcagcca ccccgccctc
     2821 cacaccacca ccgaggacag tgcaggggtg cagactgagt tcgaattctg cagtcgacgg
     2881 taccgcgggc ccgggatcca ccggccggtc gccaccatgg tgagcaaggg cgaggcagtg
     2941 atcaaggagt tcatgcggtt caaggtgcac atggagggct ccatgaacgg ccacgagttc
     3001 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
     3061 gtgaccaagg gtggccccct gcccttctcc tgggacatcc tgtcccctca gttcatgtac
     3121 ggctccaggg ccttcaccaa gcaccccgcc gacatccccg actactataa gcagtccttc
     3181 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgc cgtgaccgtg
     3241 acccaggaca cctccctgga ggacggcacc ctgatctaca aggtgaagct ccgcggcacc
     3301 aacttccctc ctgacggccc cgtaatgcag aagaagacaa tgggctggga agcgtccacc
     3361 gagcggttgt accccgagga cggcgtgctg aagggcgaca ttaagatggc cctgcgcctg
     3421 aaggacggcg gccgctacct ggcggacttc aagaccacct acaaggccaa gaagcccgtg
     3481 cagatgcccg gcgcctacaa cgtcgaccgc aagttggaca tcacctccca caacgaggac
     3541 tacaccgtgg tggaacagta cgaacgctcc gagggccgcc actccaccgg cggcatggac
     3601 gagctgtaca agtagcggcc gcgactctag atcataatca gccataccac atttgtagag
     3661 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     3721 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     3781 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     3841 ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt
     3901 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     3961 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     4021 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     4081 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     4141 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     4201 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     4261 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     4321 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     4381 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     4441 aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga
     4501 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     4561 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     4621 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     4681 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     4741 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     4801 ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
     4861 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     4921 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     4981 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg
     5041 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     5101 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     5161 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     5221 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     5281 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     5341 gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt
     5401 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     5461 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     5521 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     5581 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     5641 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
     5701 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     5761 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg
     5821 ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa
     5881 agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag
     5941 ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct
     6001 tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc
     6061 ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa
     6121 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     6181 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     6241 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     6301 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     6361 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     6421 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     6481 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     6541 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     6601 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     6661 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     6721 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     6781 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     6841 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     6901 cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcat
//
