LOCUS       V024314                 6059 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V024314
VERSION     V024314
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             625..1959
                     /label="NEK2(NM_002497)"
                     /note="NEK2(NM_002497)"
                     /gene="NEK2"
     CDS             2005..2724
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="enhanced YFP"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EYFP"
     polyA_signal    2847..2968
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(2975..3430)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3457..3561
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        3563..3920
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             3955..4749
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    4981..5028
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5357..5945
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgaggc caccatgcct tcccgggctg aggactatga agtgttgtac
      661 accattggca caggctccta cggccgctgc cagaagatcc ggaggaagag tgatggcaag
      721 atattagttt ggaaagaact tgactatggc tccatgacag aagctgagaa acagatgctt
      781 gtttctgaag tgaatttgct tcgtgaactg aaacatccaa acatcgttcg ttactatgat
      841 cggattattg accggaccaa tacaacactg tacattgtaa tggaatattg tgaaggaggg
      901 gatctggcta gtgtaattac aaagggaacc aaggaaaggc aatacttaga tgaagagttt
      961 gttcttcgag tgatgactca gttgactctg gccctgaagg aatgccacag acgaagtgat
     1021 ggtggtcata ccgtattgca tcgggatctg aaaccagcca atgttttcct ggatggcaag
     1081 caaaacgtca agcttggaga ctttgggcta gctagaatat taaaccatga cacgagtttt
     1141 gcaaaaacat ttgttggcac accttattac atgtctcctg aacaaatgaa tcgcatgtcc
     1201 tacaatgaga aatcagatat ctggtcattg ggctgcttgc tgtatgagtt atgtgcatta
     1261 atgcctccat ttacagcttt tagccagaaa gaactcgctg ggaaaatcag agaaggcaaa
     1321 ttcaggcgaa ttccataccg ttactctgat gaattgaatg aaattattac gaggatgtta
     1381 aacttaaagg attaccatcg accttctgtt gaagaaattc ttgagaaccc tttaatagca
     1441 gatttggttg cagacgagca aagaagaaat cttgagagaa gagggcgaca attaggagag
     1501 ccagaaaaat cgcaggattc cagccctgta ttgagtgagc tgaaactgaa ggaaattcag
     1561 ttacaggagc gagagcgagc tctcaaagca agagaagaaa gattggagca gaaagaacag
     1621 gagctttgtg ttcgtgagag actagcagag gacaaactgg ctagagcaga aaatctgttg
     1681 aagaactaca gcttgctaaa ggaacggaag ttcctgtctc tggcaagtaa tccagaactt
     1741 cttaatcttc catcctcagt aattaagaag aaagttcatt tcagtgggga aagtaaagag
     1801 aacatcatga ggagtgagaa ttctgagagt cagctcacat ctaagtccaa gtgcaaggac
     1861 ctgaagaaaa ggcttcacgc tgcccagctg cgggctcaag ccctgtcaga tattgagaaa
     1921 aattaccaac tgaaaagcag acagatcctg ggcatgcgcg gtggaggagg gagtggagga
     1981 ggtggaagcg gaggtggagg cagtatggtg agcaagggcg aggagctgtt caccggggtg
     2041 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     2101 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     2161 aagctgcccg tgccctggcc caccctcgtg accaccttcg gctacggcct gcagtgcttc
     2221 gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     2281 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     2341 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     2401 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     2461 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     2521 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     2581 cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccgccctgag caaagacccc
     2641 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     2701 ggcatggacg agctgtacaa gtaaagcggc cgcgactcta gatcataatc agccatacca
     2761 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     2821 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     2881 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     2941 gtttgtccaa actcatcaat gtatcttaag gcgtaaattg taagcgttaa tattttgtta
     3001 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
     3061 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
     3121 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     3181 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
     3241 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
     3301 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
     3361 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
     3421 cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     3481 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     3541 taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt
     3601 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     3661 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     3721 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     3781 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     3841 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     3901 gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt
     3961 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     4021 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     4081 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac
     4141 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     4201 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     4261 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     4321 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     4381 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     4441 caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag
     4501 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     4561 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     4621 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     4681 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     4741 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
     4801 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     4861 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     4921 ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga
     4981 cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg
     5041 ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg
     5101 cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg
     5161 cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat atactttaga
     5221 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     5281 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     5341 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     5401 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     5461 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     5521 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     5581 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     5641 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     5701 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     5761 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     5821 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     5881 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     5941 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     6001 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gccatgcat
//
