LOCUS       V031332                 5905 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031332
VERSION     V031332
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             613..1287
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="improved tetrameric variant of DsRed fluorescent
                     protein"
                     /transl_table=1
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
                     /label="DsRed2"
     CDS             1315..2568
                     /codon_start=1
                     /gene=""
                     /note=""
                     /product="human lysosome-associated membrane glycoprotein
                     1"
                     /protein_id=""
                     /transl_table=1
                     /translation="MAAPGSARRPLLLLLLLLLLGLMHCASA,AMFMVKNGNGTACIMA
                     NFSAAFSVNYDTKSGPKNMTFDLPSDATVVLNRSSCGKENTSDPSLVIAFGRGHTLTLN
                     FTRNATRYSVQLMSFVYNLSDTHLFPNASSKEIKTVESITDIRADIDKKYRCVSGTQVH
                     MNNVTVTLHDATIQAYLSNSSFSRGETRCEQDRPSPTTAPPAPPSPSPSPVPKSPSVDK
                     YNVSGTNGTCLLASMGLQLNLTYERKDNTTVTRLLNINPNKTSASGSCGAHLVTLELHS
                     EGTTVLLFQFGMNASSSRFFLQGIQLNTILPDARDPAFKAANGSLRALQATVGNSYKCN
                     AEEHVRVTKAFSVNIFKVWVQAFKVEGGQFGSVEECLLDENSMLIPIAVGGALAGLVLI
                     VLIAYLVGRKRSHAGYQTI*"
                     /label="LAMP1"
     polyA_signal    2698..2819
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(2826..3281)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3308..3412
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        3414..3771
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             3806..4600
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    4832..4879
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5208..5796
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggcctc ctccgagaac gtcatcaccg agttcatgcg cttcaaggtg
      661 cgcatggagg gcaccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
      721 ccctacgagg gccacaacac cgtgaagctg aaggtgacca agggcggccc cctgcccttc
      781 gcctgggaca tcctgtcccc ccagttccag tacggctcca aggtgtacgt gaagcacccc
      841 gccgacatcc ccgactacaa gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg
      901 atgaacttcg aggacggcgg cgtggcgacc gtgacccagg actcctccct gcaggacggc
      961 tgcttcatct acaaggtgaa gttcatcggc gtgaacttcc cctccgacgg ccccgtgatg
     1021 cagaagaaga ccatgggctg ggaggcctcc accgagcgcc tgtacccccg cgacggcgtg
     1081 ctgaagggcg agacccacaa ggccctgaag ctgaaggacg gcggccacta cctggtggag
     1141 ttcaagtcca tctacatggc caagaagccc gtgcagctgc ccggctacta ctacgtggac
     1201 gccaagctgg acatcacctc ccacaacgag gactacacca tcgtggagca gtacgagcgc
     1261 accgagggcc gccaccacct gttcctgaga tctcgagctc aagcttcgaa ttcgatggcg
     1321 gcccccggca gcgcccggcg acccctgctg ctgctactgc tgttgctgct gctcggcctc
     1381 atgcattgtg cgtcagcagc aatgtttatg gtgaaaaatg gcaacgggac cgcgtgcata
     1441 atggccaact tctctgctgc cttctcagtg aactacgaca ccaagagtgg ccctaagaac
     1501 atgacctttg acctgccatc agatgccaca gtggtgctca accgcagctc ctgtggaaaa
     1561 gagaacactt ctgaccccag tctcgtgatt gcttttggaa gaggacatac actcactctc
     1621 aatttcacga gaaatgcaac acgttacagc gtccagctca tgagttttgt ttataacttg
     1681 tcagacacac accttttccc caatgcgagc tccaaagaaa tcaagactgt ggaatctata
     1741 actgacatca gggcagatat agataaaaaa tacagatgtg ttagtggcac ccaggtccac
     1801 atgaacaacg tgaccgtaac gctccatgat gccaccatcc aggcgtacct ttccaacagc
     1861 agcttcagca ggggagagac acgctgtgaa caagacaggc cttccccaac cacagcgccc
     1921 cctgcgccac ccagcccctc gccctcaccc gtgcccaaga gcccctctgt ggacaagtac
     1981 aacgtgagcg gcaccaacgg gacctgcctg ctggccagca tggggctgca gctgaacctc
     2041 acctatgaga ggaaggacaa cacgacggtg acaaggcttc tcaacatcaa ccccaacaag
     2101 acctcggcca gcgggagctg cggcgcccac ctggtgactc tggagctgca cagcgagggc
     2161 accaccgtcc tgctcttcca gttcgggatg aatgcaagtt ctagccggtt tttcctacaa
     2221 ggaatccagt tgaatacaat tcttcctgac gccagagacc ctgcctttaa agctgccaac
     2281 ggctccctgc gagcgctgca ggccacagtc ggcaattcct acaagtgcaa cgcggaggag
     2341 cacgtccgtg tcacgaaggc gttttcagtc aatatattca aagtgtgggt ccaggctttc
     2401 aaggtggaag gtggccagtt tggctctgtg gaggagtgtc tgctggacga gaacagcatg
     2461 ctgatcccca tcgctgtggg tggtgccctg gcggggctgg tcctcatcgt cctcatcgcc
     2521 tacctcgtcg gcaggaagag gagtcacgca ggctaccaga ctatctaggg atccaccgga
     2581 tctagataac tgatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     2641 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     2701 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     2761 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa
     2821 cgcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     2881 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     2941 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     3001 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     3061 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     3121 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     3181 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     3241 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg
     3301 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     3361 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc
     3421 ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     3481 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     3541 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     3601 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     3661 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     3721 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca
     3781 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
     3841 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     3901 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     3961 cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac
     4021 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     4081 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     4141 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     4201 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     4261 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     4321 caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     4381 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     4441 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     4501 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     4561 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
     4621 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
     4681 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     4741 ggggatctca tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag
     4801 gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac
     4861 ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat
     4921 accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac
     4981 cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc
     5041 atagcctcag gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     5101 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     5161 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     5221 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     5281 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     5341 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     5401 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     5461 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     5521 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     5581 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     5641 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     5701 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     5761 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     5821 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     5881 tctgtggata accgtattac cgcca
//
