LOCUS V040921 6417 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V040921 VERSION V040921 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 61..364 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 365..568 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 613..1329 /codon_start=1 /note="mammalian codon-optimized" /product="enhanced blue variant of GFP" /transl_table=1 /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKFICTTGKLPVPWPTLVTTLTHGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV LLEFVTAAGITLGMDELYK" /label="EBFP" CDS 1360..3072 /label="KLHL28(NM_017658)" /note="KLHL28(NM_017658)" /gene="KLHL28" polyA_signal 3205..3326 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(3333..3788) /direction=LEFT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3815..3919 /gene="bla" /label="AmpR promoter" promoter 3921..4278 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4313..5107 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5339..5386 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 5715..6303 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg 661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc 721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg 781 ccctggccca ccctcgtgac caccctgacc cacggcgtgc agtgcttcag ccgctacccc 841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag 901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag 961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac 1021 atcctggggc acaagctgga gtacaacttc aacagccaca acgtctatat catggccgac 1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc 1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg 1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc 1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag 1321 ctgtacaagg gcggcggagg gagtggagga gggggaagca tggaccacac atccccgacc 1381 tacatgcttg ctaacttaac ccacttgcat tctgaacaac ttctgcaggg cttgaatctt 1441 cttcgccaac atcacgaact ctgtgacatc attcttcgag taggtgatgt taaaattcat 1501 gctcacaaag tggtacttgc cagcgtcagc ccgtatttca aagctatgtt cactggaaac 1561 ctttctgaaa aagagaacag tgaggttgag tttcaatgca ttgatgaaac tgctctccag 1621 gccattgtgg agtatgccta tacagggact gtttttattt ctcaggacac agttgaatct 1681 ctcctgccag cagcaaacct actccagata aaacttgtcc tgaaagaatg ttgtgcattt 1741 cttgaaagcc aacttgatcc tggtaattgt attggaattt ctcgttttgc agaaacatat 1801 ggttgccgtg acctttattt ggcagccact aaatacatat gccagaattt tgaagctgtt 1861 tgccagactg aagagttttt tgagcttaca catgctgact tggatgaaat tgtttccaat 1921 gactgtttga atgtagctac cgaagagact gttttttatg cattagagtc ttggatcaag 1981 tatgatgtac aagaacgcca gaaatactta gcacagttac taaacagtgt acgattacca 2041 ttgttgagtg ttaagtttct cactagacta tatgaagcaa atcatcttat tcgtgatgat 2101 cgcacttgta aacatctttt gaatgaagcc ctaaagtacc actttatgcc tgaacataga 2161 ctctctcatc agacagtctt gatgacacga cctcgctgtg ctcccaaagt actttgtgca 2221 gtaggaggga aatctggact ctttgcctgt ttggatagtg tggagatgta ctttcctcag 2281 aatgactctt ggattggttt ggcaccccta aacattcctc gctatgaatt tggaatatgc 2341 gttttagacc aaaaagtata tgttataggt ggtattgcaa ctaatgtgcg tcctggcgtc 2401 actatcagaa aacatgaaaa ttcagtggaa tgctggaatc ctgatacaaa tacttggact 2461 tctctagaga gaatgaatga aagccgaagt actcttggag tagtagtact tgcaggagaa 2521 ctttatgcct taggtggtta tgatggacaa tcttatttac aatctgtaga gaagtacatt 2581 cccaaaataa gaaaatggca acctgtggca ccaatgacga caacaagaag ttgttttgct 2641 gcagcggtat tggatggaat gatatatgcc attggtgggt atggtcctgc ccacatgaac 2701 agtgtggagc gttatgatcc aagtaaggac tcctgggaga tggttgcatc catggcagat 2761 aaaaggattc actttggcgt gggtgtcatg ctaggcttta tttttgtggt gggtggacat 2821 aatggagtct cacatttgtc cagcattgaa agatacgatc ctcatcaaaa tcagtggact 2881 gtgtgtagac caatgaaaga acctagaaca ggagttggtg ctgctgtaat cgataactac 2941 ctttatgtcg tcggtggtca ctcagggtct tcctatctga atacagtgca gaaatatgat 3001 cctatctcag atacgtggct ggattcagct ggcatgatat actgtcgctg caactttggg 3061 ttaactgcac tttgaggatc caccggatct agataactga tcataatcag ccataccaca 3121 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat 3181 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa 3241 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt 3301 ttgtccaaac tcatcaatgt atcttaacgc gtaaattgta agcgttaata ttttgttaaa 3361 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa 3421 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa 3481 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca 3541 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg 3601 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc 3661 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc 3721 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca 3781 gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt 3841 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata 3901 atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta 3961 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat 4021 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc 4081 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta 4141 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca 4201 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga 4261 ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga 4321 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga 4381 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg 4441 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga 4501 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt 4561 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct 4621 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct 4681 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg 4741 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca 4801 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga 4861 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt 4921 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt 4981 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct 5041 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt 5101 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca 5161 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg 5221 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct 5281 agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg 5341 gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt 5401 cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc 5461 cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca 5521 gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat actttagatt 5581 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc 5641 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag 5701 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa 5761 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg 5821 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag 5881 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg 5941 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga 6001 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc 6061 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc 6121 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga 6181 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt 6241 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg 6301 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac 6361 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc catgcat //