LOCUS       V021682                 8188 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V021682
VERSION     V021682
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             624..4100
                     /label="KCNH2(NM_000238)"
                     /note="KCNH2(NM_000238)"
                     /gene="KCNH2"
     CDS             4134..4853
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     polyA_signal    4976..5097
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(5104..5559)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5586..5690
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        5692..6049
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             6084..6878
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    7110..7157
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      7486..8074
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagcc accatgccgg tgcggagggg ccacgtcgcg ccgcagaaca
      661 ccttcctgga caccatcatc cgcaagtttg agggccagag ccgtaagttc atcatcgcca
      721 acgctcgggt ggagaactgc gccgtcatct actgcaacga cggcttctgc gagctgtgcg
      781 gctactcgcg ggccgaggtg atgcagcgac cctgcacctg cgacttcctg cacgggccgc
      841 gcacgcagcg ccgcgctgcc gcgcagatcg cgcaggcact gctgggcgcc gaggagcgca
      901 aagtggaaat cgccttctac cggaaagatg ggagctgctt cctatgtctg gtggatgtgg
      961 tgcccgtgaa gaacgaggat ggggctgtca tcatgttcat cctcaatttc gaggtggtga
     1021 tggagaagga catggtgggg tccccggctc atgacaccaa ccaccggggc ccccccacca
     1081 gctggctggc cccaggccgc gccaagacct tccgcctgaa gctgcccgcg ctgctggcgc
     1141 tgacggcccg ggagtcgtcg gtgcggtcgg gcggcgcggg cggcgcgggc gccccggggg
     1201 ccgtggtggt ggacgtggac ctgacgcccg cggcacccag cagcgagtcg ctggccctgg
     1261 acgaagtgac agccatggac aaccacgtgg cagggctcgg gcccgcggag gagcggcgtg
     1321 cgctggtggg tcccggctct ccgccccgca gcgcgcccgg ccagctccca tcgccccggg
     1381 cgcacagcct caaccccgac gcctcgggct ccagctgcag cctggcccgg acgcgctccc
     1441 gagaaagctg cgccagcgtg cgccgcgcct cgtcggccga cgacatcgag gccatgcgcg
     1501 ccggggtgct gcccccgcca ccgcgccacg ccagcaccgg ggccatgcac ccactgcgca
     1561 gcggcttgct caactccacc tcggactccg acctcgtgcg ctaccgcacc attagcaaga
     1621 ttccccaaat caccctcaac tttgtggacc tcaagggcga ccccttcttg gcttcgccca
     1681 ccagtgaccg tgagatcata gcacctaaga taaaggagcg aacccacaat gtcactgaga
     1741 aggtcaccca ggtcctgtcc ctgggcgccg acgtgctgcc tgagtacaag ctgcaggcac
     1801 cgcgcatcca ccgctggacc atcctgcatt acagcccctt caaggccgtg tgggactggc
     1861 tcatcctgct gctggtcatc tacacggctg tcttcacacc ctactcggct gccttcctgc
     1921 tgaaggagac ggaagaaggc ccgcctgcta ccgagtgtgg ctacgcctgc cagccgctgg
     1981 ctgtggtgga cctcatcgtg gacatcatgt tcattgtgga catcctcatc aacttccgca
     2041 ccacctacgt caatgccaac gaggaggtgg tcagccaccc cggccgcatc gccgtccact
     2101 acttcaaggg ctggttcctc atcgacatgg tggccgccat ccccttcgac ctgctcatct
     2161 tcggctctgg ctctgaggag ctgatcgggc tgctgaagac tgcgcggctg ctgcggctgg
     2221 tgcgcgtggc gcggaagctg gatcgctact cagagtacgg cgcggccgtg ctgttcttgc
     2281 tcatgtgcac ctttgcgctc atcgcgcact ggctagcctg catctggtac gccatcggca
     2341 acatggagca gccacacatg gactcacgca tcggctggct gcacaacctg ggcgaccaga
     2401 taggcaaacc ctacaacagc agcggcctgg gcggcccctc catcaaggac aagtatgtga
     2461 cggcgctcta cttcaccttc agcagcctca ccagtgtggg cttcggcaac gtctctccca
     2521 acaccaactc agagaagatc ttctccatct gcgtcatgct cattggctcc ctcatgtatg
     2581 ctagcatctt cggcaacgtg tcggccatca tccagcggct gtactcgggc acagcccgct
     2641 accacacaca gatgctgcgg gtgcgggagt tcatccgctt ccaccagatc cccaatcccc
     2701 tgcgccagcg cctcgaggag tacttccagc acgcctggtc ctacaccaac ggcatcgaca
     2761 tgaacgcggt gctgaagggc ttccctgagt gcctgcaggc tgacatctgc ctgcacctga
     2821 accgctcact gctgcagcac tgcaaaccct tccgaggggc caccaagggc tgccttcggg
     2881 ccctggccat gaagttcaag accacacatg caccgccagg ggacacactg gtgcatgctg
     2941 gggacctgct caccgccctg tacttcatct cccggggctc catcgagatc ctgcggggcg
     3001 acgtcgtcgt ggccatcctg gggaagaatg acatctttgg ggagcctctg aacctgtatg
     3061 caaggcctgg caagtcgaac ggggatgtgc gggccctcac ctactgtgac ctacacaaga
     3121 tccatcggga cgacctgctg gaggtgctgg acatgtaccc tgagttctcc gaccacttct
     3181 ggtccagcct ggagatcacc ttcaacctgc gagataccaa catgatcccg ggctcccccg
     3241 gcagtacgga gttagagggt ggcttcagtc ggcaacgcaa gcgcaagttg tccttccgca
     3301 ggcgcacgga caaggacacg gagcagccag gggaggtgtc ggccttgggg ccgggccggg
     3361 cgggggcagg gccgagtagc cggggccggc cgggggggcc gtggggggag agcccgtcca
     3421 gtggcccctc cagccctgag agcagtgagg atgagggccc aggccgcagc tccagccccc
     3481 tccgcctggt gcccttctcc agccccaggc cccccggaga gccgccgggt ggggagcccc
     3541 tgatggagga ctgcgagaag agcagcgaca cttgcaaccc cctgtcaggc gccttctcag
     3601 gagtgtccaa cattttcagc ttctgggggg acagtcgggg ccgccagtac caggagctcc
     3661 ctcgatgccc cgcccccacc cccagcctcc tcaacatccc cctctccagc ccgggtcggc
     3721 ggccccgggg cgacgtggag agcaggctgg atgccctcca gcgccagctc aacaggctgg
     3781 agacccggct gagtgcagac atggccactg tcctgcagct gctacagagg cagatgacgc
     3841 tggtcccgcc cgcctacagt gctgtgacca ccccggggcc tggccccact tccacatccc
     3901 cgctgttgcc cgtcagcccc ctccccaccc tcaccttgga ctcgctttct caggtttccc
     3961 agttcatggc gtgtgaggag ctgcccccgg gggccccaga gcttccccaa gaaggcccca
     4021 cacgacgcct ctccctaccg ggccagctgg gggccctcac ctcccagccc ctgcacagac
     4081 acggctcgga cccgggcagt ggaggagggg gaagcgatcc accggtcgcc accatggtga
     4141 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     4201 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     4261 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     4321 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     4381 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     4441 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     4501 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     4561 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     4621 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     4681 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     4741 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     4801 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag taaagcggcc
     4861 gcgactctag atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
     4921 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
     4981 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     5041 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg
     5101 cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
     5161 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
     5221 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
     5281 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
     5341 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
     5401 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
     5461 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
     5521 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa
     5581 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     5641 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg
     5701 aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     5761 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     5821 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     5881 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     5941 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     6001 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag
     6061 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg
     6121 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
     6181 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
     6241 tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga
     6301 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
     6361 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
     6421 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
     6481 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
     6541 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
     6601 ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
     6661 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
     6721 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
     6781 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
     6841 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat
     6901 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
     6961 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     7021 ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga
     7081 gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg
     7141 tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac
     7201 cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc
     7261 cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat
     7321 agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     7381 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     7441 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     7501 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     7561 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     7621 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     7681 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     7741 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     7801 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     7861 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     7921 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     7981 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     8041 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     8101 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     8161 tgtggataac cgtattaccg ccatgcat
//
