LOCUS       V018290                 8214 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V018290
VERSION     V018290
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             613..1320
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             1357..4869
                     /label="Grm5(NM_001081414)"
                     /note="Grm5(NM_001081414)"
                     /gene="Grm5"
     polyA_signal    5002..5123
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(5130..5585)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5612..5716
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        5718..6075
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             6110..6904
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    7136..7183
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      7512..8100
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
      661 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
      721 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
      781 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
      841 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
      901 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
      961 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     1021 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     1081 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     1141 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     1201 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     1261 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     1321 tccggactca gatctcgagc tcaagcttcg aattctatgg tccttctgtt gattctgtca
     1381 gtcctacttc tgaaagaaga tgtacgagga agtgcacagt ccagtgagag gagggtggtg
     1441 gctcacatgc caggtgacat tattattgga gctctcttct cggtccacca ccaaccaact
     1501 gtggacaaag ttcatgagag gaagtgtggg gcagtccgtg agcagtatgg cattcagaga
     1561 gtggaagcca tgctgcatac cttggaaagg atcaattcag atcccacact cttgcccaac
     1621 atcacactag gctgtgagat aagagattcc tgctggcatt ctgctgtggc cctagagcaa
     1681 agcattgagt ttataaggga ttccctcatc tcttcggaag aggaagaagg cttggtacgc
     1741 tgtgtagatg gctcttcttc cttccgctcc aagaaaccca tagtgggagt cattgggcct
     1801 ggctcgagtt ctgtggccat tcaagttcag aacttgctcc agcttttcaa catacctcag
     1861 attgcttact ctgcaactag catggatttg agtgacaaga ctctattcaa gtacttcatg
     1921 agggttgtac cttcggatgc ccagcaagcc cgagccatgg tagacatagt gaagagatac
     1981 aactggactt atgtctcagc tgtgcacaca gaaggcaact atggagaaag tgggatggag
     2041 gctttcaaag atatgtcagc gaaggaaggg atttgcatcg cccactctta caaaatctac
     2101 agcaatgctg gggaacagag ctttgacaag ctgttgaaaa agctcagaag tcatttacct
     2161 aaagcccggg tggtagcctg cttctgtgaa ggcatgacag ttcgaggtct gctcatggcc
     2221 atgagacgct tgggtctagc aggggaattt ctacttctgg gcagtgatgg ctgggctgac
     2281 aggtatgacg tgacagatgg gtatcagcga gaagctgtcg gtgggattac aatcaagctc
     2341 cagtctcctg atgtcaagtg gtttgatgat tattatctga agctccggcc agaaacaaac
     2401 ctcagaaacc cttggtttca agaattttgg cagcatcgtt ttcagtgccg gctagaaggg
     2461 tttgcacagg agaacagcaa gtacaacaag acttgcaaca gttctctaac tctgagaacg
     2521 catcatgttc aagattccaa aatgggattt gtgatcaatg caatctattc tatggcttat
     2581 gggctccaca acatgcagat gtccctgtgt ccaggctatg caggcctctg tgatgcaatg
     2641 aagccaattg atgggcggaa acttttggac tccctgatga aaaccaactt tactggagtt
     2701 tccggagata tgattctatt tgatgaaaat ggagactctc caggaaggta tgaaataatg
     2761 aatttcaagg aaatgggaaa agattatttt gattacatca atgttggaag ttgggacaat
     2821 ggggaattaa aaatggatga tgatgaagtg tggtccaaga aaaataacat catcagatct
     2881 gtgtgcagtg aaccgtgtga gaaaggacag ataaaggtga tccggaaggg agaagtcagc
     2941 tgttgttgga cctgcacacc ttgtaaggag aatgagtatg tttttgatga gtacacctgc
     3001 aaggcgtgcc aactggggtc ctggcccact gacgacttga caggttgtga tttgatccca
     3061 gtccagtatc ttcgatgggg tgaccctgag cccattgcag ctgtggtgtt tgcctgcctc
     3121 ggtctgctag ccaccctctt cgttactgta atcttcatca tttatcggga cactccagtg
     3181 gtcaagtctt ccagcaggga actctgctac attatccttg ctggcatctg cctgggttac
     3241 ttgtgtacct tctgcctcat tgcaaagccc aaacagattt actgctacct tcagagaatt
     3301 ggcatcggtc tctctccagc catgagctac tcagcccttg taacgaagac caaccgtatt
     3361 gcaaggattc tagctggcag caagaagaag atctgtacca aaaagcccag attcatgagc
     3421 gcctgtgctc agttagtgat cgctttcatt ctcatctgta tacagttggg tattattgtg
     3481 gccctcttta tcatggagcc tccggatata atgcatgact atccaagcat ccgagaagtc
     3541 tacttgattt gtaacaccac caacctaggg gttgtcactc ctcttggata caatggatta
     3601 ttgattttga gttgcacatt ctatgcgttc aagaccagaa atgttccagc caactttaac
     3661 gaggccaaat atattgcttt caccatgtac acaacctgca tcatatggct ggcctttgtg
     3721 cctatctact ttggcagcaa ctacaaaatc atcaccatgt gtttctcagt cagcctcagt
     3781 gccacagtgg ccctgggttg catgtttgtg ccgaaggtgt acatcatcct agccaaaccg
     3841 gagagaaatg tgcgcagcgc cttcacaacc tctacagtgg tgcgcatgca cgtaggagat
     3901 ggcaagtcat catccgctgc cagcagatcc agcagcctag tcaacctgtg gaagaggagg
     3961 ggctcgtctg gggaaaccct aagctccaat ggaaaatccg tgacttgggc ccagaatgag
     4021 aagagcaccc gggggcagca cctgtggcag cgactgtctg tccatatcaa caagaaggag
     4081 aaccccaacc agacagcagt catcaaaccc ttccccaaga gcacagagag ccgcgggcag
     4141 ggtgcagggg caggtggtgg ctctggcccc ggtgcagctg gtgctggtag cgcaggatgc
     4201 acagcgacag gcggcccaga gccaccagac gccggcccca aggcgcttta tgatgtcgca
     4261 gaggcagagg agcgcttccc agcggctgcc aggccgcgct cgccatcgcc catcagtacg
     4321 ctgagccacc tggcaggctc ggcgggccgc acagacgacg acgcgccgtc gctgcactcg
     4381 gagaccgctg cacgcagcag ctcatcccag ggctcgctca tggagcagat tagcagcgtg
     4441 gtgacgcgct tcaccgccaa catcaccgag ctcaactcca tgatgttgtc caccgcggct
     4501 gcgccggggc cccctggtac ccctatctgc tcttcctacc tgatccccaa agagatccag
     4561 ctgcccacga ccatgacgac cttcgcagag atccagcctc tgccggccat cgaggtgacc
     4621 ggaggagctc agccggcgac agggccatca cctgcccaag agacgcccgc aggagctgaa
     4681 gccgccccag gaaaaccgga tctggaggag ctggtggccc tcactccacc atcgcccttc
     4741 agggactcgg tggactcggg gagcaccacc ccaaactctc cagtctccga atcggccctc
     4801 tgcatcccat cctctcccaa atatgacact ctcatcatca gagattacac gcagagttct
     4861 tcatcgttgt gaggatccac cggatctaga taactgatca taatcagcca taccacattt
     4921 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     4981 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     5041 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     5101 tccaaactca tcaatgtatc ttaacgcgta aattgtaagc gttaatattt tgttaaaatt
     5161 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
     5221 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
     5281 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     5341 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
     5401 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     5461 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
     5521 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     5581 cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     5641 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     5701 ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg
     5761 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     5821 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     5881 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     5941 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     6001 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     6061 ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca tgattgaaca
     6121 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     6181 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     6241 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc
     6301 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     6361 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     6421 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     6481 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     6541 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     6601 gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct
     6661 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     6721 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     6781 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     6841 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     6901 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga
     6961 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     7021 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccctagg
     7081 gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca
     7141 ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg
     7201 tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc
     7261 gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc
     7321 aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact ttagattgat
     7381 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     7441 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     7501 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     7561 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     7621 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     7681 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     7741 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     7801 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     7861 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     7921 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     7981 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     8041 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     8101 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     8161 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat gcat
//
