LOCUS       V020190                 5794 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V020190
VERSION     V020190
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             627..653
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             654..1706
                     /label="GPD1L(NM_015141)"
                     /note="GPD1L(NM_015141)"
                     /gene="GPD1L"
     CDS             1740..2459
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     polyA_signal    2582..2703
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(2710..3165)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3192..3296
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        3298..3655
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             3690..4484
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    4716..4763
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5092..5680
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagcc accatgtacc catacgacgt accagattac gctatggcag
      661 cggcgcccct gaaagtgtgc atcgtgggct cggggaactg gggttcagct gttgcaaaaa
      721 taattggtaa taatgtcaag aaacttcaga aatttgcctc cacagtcaag atgtgggtct
      781 ttgaagaaac agtgaatggc agaaaactga cagacatcat aaataatgac catgaaaatg
      841 taaaatatct tcctggacac aagctgccag aaaatgtggt tgccatgtca aatcttagcg
      901 aggctgtgca ggatgcagac ctgctggtgt ttgtcattcc ccaccagttc attcacagaa
      961 tctgtgatga gatcactggg agagtgccca agaaagcgct gggaatcacc ctcatcaagg
     1021 gcatagacga gggccccgag gggctgaagc tcatttctga catcatccgt gagaagatgg
     1081 gtattgacat cagtgtgctg atgggagcca acattgccaa tgaggtggct gcagagaagt
     1141 tctgtgagac caccatcggc agcaaagtaa tggagaacgg ccttctcttc aaagaacttc
     1201 tgcagactcc aaattttcga attaccgtgg ttgatgatgc agacactgtt gaactctgtg
     1261 gtgcgcttaa gaacatcgta gctgtgggag ctgggttctg cgacggcctc cgctgtggag
     1321 acaacaccaa agcggccgtc atccgcctgg gactcatgga aatgattgct tttgccagga
     1381 tcttctgcaa aggccaagtg tctacagcca ccttcctaga gagctgcggg gtggccgacc
     1441 tgatcaccac ctgttacgga gggcggaacc gcagggtggc cgaggccttc gccagaactg
     1501 ggaagaccat tgaagagttg gagaaggaga tgctgaatgg gcaaaagctc caaggaccgc
     1561 agacttctgc tgaagtgtac cgcatcctca aacagaaggg actactggac aagtttccat
     1621 tgtttactgc agtgtatcag atctgctacg aaagcagacc agttcaagag atgttgtctt
     1681 gtcttcagag ccatccagag catacaggag gagggggaag cgatccaccg gtcgccacca
     1741 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     1801 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     1861 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     1921 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
     1981 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     2041 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     2101 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     2161 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     2221 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     2281 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     2341 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     2401 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaaa
     2461 gcggccgcga ctctagatca taatcagcca taccacattt gtagaggttt tacttgcttt
     2521 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     2581 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     2641 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     2701 ttaaggcgta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     2761 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     2821 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     2881 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
     2941 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
     3001 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
     3061 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
     3121 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc
     3181 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     3241 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg
     3301 aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     3361 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     3421 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     3481 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     3541 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     3601 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg
     3661 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
     3721 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     3781 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     3841 ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg
     3901 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     3961 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     4021 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     4081 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     4141 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     4201 tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     4261 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     4321 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     4381 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     4441 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
     4501 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
     4561 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     4621 gcgcggggat ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg
     4681 gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac
     4741 gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt
     4801 cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc
     4861 ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc
     4921 tgccatagcc tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt
     4981 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     5041 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     5101 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     5161 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     5221 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     5281 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     5341 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     5401 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     5461 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     5521 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     5581 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     5641 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     5701 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     5761 tgattctgtg gataaccgta ttaccgccat gcat
//
