LOCUS V044643 7346 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V044643 VERSION V044643 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 61..364 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 365..568 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 613..1320 /codon_start=1 /note="mammalian codon-optimized" /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /transl_table=1 /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" /label="mCherry" CDS 1378..3906 /label="FBXO11(NM_025133)" /note="FBXO11(NM_025133)" /gene="FBXO11" CDS 3913..4002 /codon_start=1 /product="3 tandem Myc epitope tags" /transl_table=1 /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL" /label="3xMyc" polyA_signal 4134..4255 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(4262..4717) /direction=LEFT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 4744..4848 /gene="bla" /label="AmpR promoter" promoter 4850..5207 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 5242..6036 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 6268..6315 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 6644..7232 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc 661 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc 721 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt 781 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc 841 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc 901 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc 961 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc 1021 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac 1081 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc 1141 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc 1201 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg 1261 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag 1321 tccggactca gatctcgagc tggaggtgga ggtagtggag gaggcggaag caagcttatg 1381 gttgcagaag aatcaggtcc tggtgcacaa aatagtccat accaacttcg tagaaaaact 1441 cttttgccga aaagaacagc gtgtcccaca aagaacagta tggagggcgc ctcaacttca 1501 actacagaaa actttggtca tcgtgcaaaa cgtgcaagag tgtctggaaa atcacaagat 1561 ctatcagcag cacctgctga acagtatctt caggagaaac tgccagatga agtggttcta 1621 aaaatcttct cttacttgct ggaacaggat ctttgtagag cagcttgtgt atgtaaacgc 1681 ttcagtgaac ttgctaatga tccaattttg tggaaacgat tatatatgga agtatttgaa 1741 tatactcgcc ctatgatgca tcctgaacct ggaaaattct accagattaa tccagaagag 1801 tatgaacatc caaatccctg gaaagagagt ttccagcagt tgtataaagg tgcacatgta 1861 aagccaggat ttgctgaaca tttctacagt aaccctgcaa gatataaagg aagagaaaat 1921 atgttgtatt atgatactat tgaagatgcc cttggtgggg tacaagaggc tcattttgat 1981 ggacttatct ttgttcattc tggaatatat actgatgaat ggatatatat tgaatctcca 2041 atcaccatga ttggtgcagc acctgggaaa gtggcagaca aagttataat tgaaaacact 2101 agagattcaa ccttcgtttt tatggaaggc tctgaagatg cttatgttgg atatatgaca 2161 ataaggttta accctgatga caaatctgca caacaccaca atgcacacca ctgcttagag 2221 attacagtaa attgtagccc tattattgat cactgtatca tccgaagtac atgtacagtt 2281 ggttctgcag tatgtgttag tggtcaagga gcatgtccca ccatcaagca ctgtaacatc 2341 agtgactgtg aaaatgttgg actatatata acagatcatg cacagggaat atatgaggat 2401 aatgaaattt ccaataatgc gttagctggg atttgggtta aaaatcatgg aaacccaatt 2461 attagacgga atcatattca tcatggacgt gatgttggtg tgttcacatt tgatcatggc 2521 atgggttact ttgaaagttg caatatacac agaaatagga tagcaggctt tgaagtaaaa 2581 gcctatgcta accctacagt ggttcgatgt gaaattcacc atgggcagac tggaggaata 2641 tatgtccatg aaaaaggaag aggacaattc atagagaata aaatctatgc aaacaacttt 2701 gcaggtgtat ggattacctc aaatagtgac ccaacaataa ggggaaattc tatatttaat 2761 ggaaatcaag gaggagttta catctttggt gatggacgag gccttattga aggaaatgac 2821 atttatggca atgcattagc aggaattcaa attaggacaa acagttgtcc aattgttcgg 2881 cataacaaaa ttcatgatgg ccagcatggt gggatttatg tgcatgaaaa gggacaagga 2941 gtaatagaag agaatgaagt ttatagtaac actctagctg gagtctgggt gacaactggc 3001 agcactccag tactgagaag aaaccggata cacagtggca agcaggttgg tgtttatttt 3061 tatgacaatg gacatggagt gctagaagac aatgatatct ataatcatat gtattcaggg 3121 gttcagataa ggactggaag caaccccaaa attagacgca acaaaatctg gggaggacag 3181 aatggtggaa ttctagttta taattctggt ctaggctgta tagaagacaa tgaaatattt 3241 gacaatgcaa tggctggagt ctggattaag acagatagta atcctacact aagaagaaat 3301 aaaatccatg atggaagaga tggtggcatc tgtatattta atgggggtcg aggtctcctt 3361 gaagaaaatg atattttcag gaatgctcaa gcaggtgttc tcatcagcac taatagtcat 3421 ccaatcttaa ggaaaaacag aatatttgat ggatttgccg caggtattga aattacaaat 3481 cacgcaactg caacactaga aggcaatcag atttttaaca accggtttgg aggcttattt 3541 ttagcatctg gtgttaatgt gacaatgaaa gataacaaaa taatgaacaa tcaagatgcc 3601 atagaaaagg ctgttagtag aggccaatgt ttatataaaa tatcaagtta taccagctat 3661 cccatgcatg atttctacag atgtcatact tgtaacacca cagatcgaaa tgccatatgt 3721 gtgaactgca ttaagaagtg ccatcaggga catgatgtag agtttattag acatgatagg 3781 tttttctgtg actgtggtgc tggaacactg tctaatcctt gtacattagc tggtgagcct 3841 acacatgata cagatacact atatgactct gctccaccta tagaatctaa tacattgcag 3901 cacaacgaat tcgaacaaaa actaatatca gaggaagatt tggaacagaa actaatcagt 3961 gaagaagatt tagagcaaaa gttaatttcc gaggaggact tataggatcc accggatcta 4021 gataactgat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc 4081 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt 4141 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag 4201 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttaacgcg 4261 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca 4321 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag 4381 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc 4441 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc 4501 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc 4561 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa 4621 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc 4681 acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat 4741 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg 4801 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa 4861 agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag 4921 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag 4981 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc 5041 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc 5101 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat 5161 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat cgatcaagag 5221 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc 5281 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat 5341 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg 5401 tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg 5461 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta 5521 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta 5581 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc 5641 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc 5701 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg 5761 ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg 5821 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt 5881 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc 5941 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc 6001 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga 6061 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg 6121 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg 6181 atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca cggaaggaga 6241 caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacggtg 6301 ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc 6361 caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc 6421 caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag 6481 cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga 6541 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt 6601 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc 6661 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc 6721 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac 6781 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac 6841 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt 6901 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct 6961 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat 7021 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt 7081 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg 7141 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt 7201 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt 7261 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg 7321 tggataaccg tattaccgcc atgcat //