LOCUS       V026698                 7548 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026698
VERSION     V026698
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             625..3471
                     /label="EPHA4(NM_001363748)"
                     /note="EPHA4(NM_001363748)"
                     /gene="EPHA4"
     CDS             3505..4215
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK*"
                     /label="mCherry"
     polyA_signal    4336..4457
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(4464..4919)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4946..5050
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        5052..5409
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5444..6238
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6470..6517
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      6846..7434
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgaggc caccatggct gggattttct atttcgccct attttcgtgt
      661 ctcttcggga tttgcgacgc tgtcacaggt tccagggtat accccgcgaa tgaagttacc
      721 ttattggatt ccagatctgt tcagggagaa cttgggtgga tagcaagccc tctggaagga
      781 gggtgggagg aagtgagtat catggatgaa aaaaatacac caatccgaac ctaccaagtg
      841 tgcaatgtga tggaacccag ccagaataac tggctacgaa ctgattggat cacccgagaa
      901 ggggctcaga gggtgtatat tgagattaaa ttcaccttga gggactgcaa tagtcttccg
      961 ggcgtcatgg ggacttgcaa ggagacgttt aacctgtact actatgaatc agacaacgac
     1021 aaagagcgtt tcatcagaga gaaccagttt gtcaaaattg acaccattgc tgctgatgag
     1081 agcttcaccc aagtggacat tggtgacaga atcatgaagc tgaacaccga gatccgggat
     1141 gtagggccat taagcaaaaa ggggttttac ctggcttttc aggatgtggg ggcctgcatc
     1201 gccctggtat cagtccgtgt gttctataaa aagtgtccac tcacagtccg caatctggcc
     1261 cagtttcctg acaccatcac aggggctgat acgtcttccc tggtggaagt tcgaggctcc
     1321 tgtgtcaaca actcagaaga gaaagatgtg ccaaaaatgt actgtggggc agatggtgaa
     1381 tggctggtac ccattggcaa ctgcctatgc aacgctgggc atgaggagcg gagcggagaa
     1441 tgccaagctt gcaaaattgg atattacaag gctctctcca cggatgccac ctgtgccaag
     1501 tgcccacccc acagctactc tgtctgggaa ggagccacct cgtgcacctg tgaccgaggc
     1561 tttttcagag ctgacaacga tgctgcctct atgccctgca cccgtccacc atctgctccc
     1621 ctgaacttga tttcaaatgt caacgagaca tctgtgaact tggaatggag tagccctcag
     1681 aatacaggtg gccgccagga catttcctat aatgtggtat gcaagaaatg tggagctggt
     1741 gaccccagca agtgccgacc ctgtggaagt ggggtccact acaccccaca gcagaatggc
     1801 ttgaagacca ccaaagtctc catcactgac ctcctagctc ataccaatta cacctttgaa
     1861 atctgggctg tgaatggagt gtccaaatat aaccctaacc cagaccaatc agtttctgtc
     1921 actgtgacca ccaaccaagc agcaccatca tccattgctt tggtccaggc taaagaagtc
     1981 acaagataca gtgtggcact ggcttggctg gaaccagatc ggcccaatgg ggtaatcctg
     2041 gaatatgaag tcaagtatta tgagaaggat cagaatgagc gaagctatcg tatagttcgg
     2101 acagctgcca ggaacacaga tatcaaaggc ctgaaccctc tcacttccta tgttttccac
     2161 gtgcgagcca ggacagcagc tggctatgga gacttcagtg agcccttgga ggttacaacc
     2221 aacacagtgc cttcccggat cattggagat ggggctaact ccacagtcct tctggtctct
     2281 gtctcgggca gtgtggtgct ggtggtaatt ctcattgcag cttttgtcat cagccggaga
     2341 cggagtaaat acagtaaagc caaacaagaa gcggatgaag agaaacattt gaatcaaggt
     2401 gtaagaacat atgtggaccc ctttacgtac gaagatccca accaagcagt gcgagagttt
     2461 gccaaagaaa ttgacgcatc ctgcattaag attgaaaaag ttataggagt tggtgaattt
     2521 ggtgaggtat gcagtgggcg tctcaaagtg cctggcaaga gagagatctg tgtggctatc
     2581 aagactctga aagctggtta tacagacaaa cagaggagag acttcctgag tgaggccagc
     2641 atcatgggac agtttgacca tccgaacatc attcacttgg aaggcgtggt cactaaatgt
     2701 aaaccagtaa tgatcataac agagtacatg gagaatggct ccttggatgc attcctcagg
     2761 aaaaatgatg gcagatttac agtcattcag ctggtgggca tgcttcgtgg cattgggtct
     2821 gggatgaagt atttatctga tatgagctat gtgcatcgtg atctggccgc acggaacatc
     2881 ctggtgaaca gcaacttggt ctgcaaagtg tctgattttg gcatgtcccg agtgcttgag
     2941 gatgatccgg aagcagctta caccaccagg ggtggcaaga ttcctatccg gtggactgcg
     3001 ccagaagcaa ttgcctatcg taaattcaca tcagcaagtg atgtatggag ctatggaatc
     3061 gttatgtggg aagtgatgtc gtacggggag aggccctatt gggatatgtc caatcaagat
     3121 gtgattaaag ccattgagga aggctatcgg ttaccccctc caatggactg ccccattgcg
     3181 ctccaccagc tgatgctaga ctgctggcag aaggagagga gcgacaggcc taaatttggg
     3241 cagattgtca acatgttgga caaactcatc cgcaacccca acagcttgaa gaggacaggg
     3301 acggagagct ccagacctaa cactgccttg ttggatccaa gctcccctga attctctgct
     3361 gtggtatcag tgggcgattg gctccaggcc attaaaatgg accggtataa ggataacttc
     3421 acagctgctg gttataccac actagaggct gtggtgcacg tgaaccagga gggaggtgga
     3481 ggttcggatc caccggtcgc caccatggtg agcaagggcg aggaggataa catggccatc
     3541 atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc
     3601 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
     3661 gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac
     3721 ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc
     3781 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg
     3841 acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc
     3901 aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc
     3961 gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg
     4021 aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg
     4081 cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac
     4141 tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac
     4201 gagctgtaca agtagcggcc gcgactctag atcataatca gccataccac atttgtagag
     4261 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     4321 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     4381 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     4441 ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt
     4501 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     4561 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     4621 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     4681 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     4741 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     4801 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     4861 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     4921 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     4981 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     5041 aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga
     5101 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     5161 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     5221 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     5281 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     5341 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     5401 ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
     5461 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     5521 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     5581 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg
     5641 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     5701 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     5761 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     5821 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     5881 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     5941 gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt
     6001 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     6061 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     6121 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     6181 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     6241 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
     6301 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     6361 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg
     6421 ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa
     6481 agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag
     6541 ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct
     6601 tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc
     6661 ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa
     6721 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     6781 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     6841 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     6901 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     6961 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     7021 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     7081 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     7141 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     7201 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     7261 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     7321 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     7381 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     7441 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     7501 cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcat
//
