LOCUS       V018874                 6710 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V018874
VERSION     V018874
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             625..2559
                     /label="DYNC1I1(NM_004411)"
                     /note="DYNC1I1(NM_004411)"
                     /gene="DYNC1I1"
     CDS             2656..3375
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     polyA_signal    3498..3619
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3626..4081)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4108..4212
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        4214..4571
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4606..5400
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5632..5679
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      6008..6596
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgaggc caccatgtct gacaaaagtg acttaaaagc tgagctagag
      661 cgcaaaaagc agcgcttagc acagataaga gaagagaaga aacggaagga agaggagagg
      721 aaaaagaaag aggctgatat gcagcagaag aaagaacccg ttcaggacga ctctgatctg
      781 gatcgcaaac gacgagagac agaggctttg ctgcaaagca ttggtatctc accggagccg
      841 cctctagtgc agccgctgca ttttttaaca tgggatacct gttattttca ttatttagtc
      901 ccaaccccta tgtctccctc ctcgaaatca gtgagcactc ccagtgaagc tggaagccaa
      961 gactcaggcg atctggggcc attaacaagg accctgcagt gggacacaga cccctcagtg
     1021 ctccagctgc agtcagactc agaacttgga agaagactgc ataaactggg cgtgtcaaag
     1081 gtcacccaag tggatttcct gccaagggaa gtagtgtcct actcaaagga gacccagact
     1141 cctcttgcca cgcatcagtc tgaagaggat gaggaagatg aggaaatggt ggaatctaaa
     1201 gttggccagg actcagaact ggaaaatcag gacaaaaaac aggaagtgaa ggaagcccct
     1261 ccaagagagt tgacagagga agaaaaacag cagatccttc attcagagga atttctcatc
     1321 ttttttgacc ggacaatacg ggtaattgaa agagccctgg ctgaagattc cgacatcttt
     1381 tttgactaca gcggccgaga gttagaggaa aaagatgggg atgttcaggc tggagccaat
     1441 ctttctttca atcgtcagtt ctatgatgaa cattggtcca agcatcgagt ggtcacttgt
     1501 atggactggt ccctccagta ccctgagctg atggtggctt cttacaacaa caatgaagat
     1561 gctccccatg aaccagatgg agtggccttg gtttggaaca tgaagtttaa gaaaaccaca
     1621 ccagaatacg tcttccactg tcagtcctct gtgatgtcgg tctgcttcgc ccgtttccat
     1681 cctaacttgg tggttggtgg gacttactcg ggccagattg tcctctggga caatcgcagt
     1741 catcgaagga ctccagtgca gcggacaccc ttatcagctg ctgcacacac gcatcccgtg
     1801 tactgtgtaa atgttgttgg gacccagaat gctcataacc tcatcactgt ctccactgat
     1861 ggcaaaatgt gttcctggag cctggacatg ctctcaactc cacaggagag catggagctg
     1921 gtgtacaata agtccaagcc tgtcgctgtt accggaatgg ctttcccaac gggagacgtc
     1981 aataacttcg tggttggcag tgaggaaggt acagtctaca cggcttgtcg tcatggaagc
     2041 aaagcaggta ttggtgaggt ctttgaaggt caccaagggc cagtgacagg aattaactgc
     2101 cacatggcag tgggcccaat cgacttttct cacctgtttg tcacatcatc atttgactgg
     2161 actgtcaaac tgtggaccac caagcacaac aagccgctct actcctttga agacaatgca
     2221 gactatgtgt acgatgtcat gtggtccccc gtgcatcctg cgctttttgc ctgcgtggac
     2281 gggatggggc gcttggacct ctggaacctc aacaatgaca ccgaggttcc aacagcaagt
     2341 gtggccattg agggggcatc cgccctaaac cgtgttcgtt gggcccaagc tggcaaagaa
     2401 gttgctgttg gggactcgga aggccgtatt tgggtctatg acgttggaga gcttgcagtt
     2461 ccccacaatg atgaatggac ccgatttgcc aggacccttg tggaaattcg tgctaacaga
     2521 gctgatagcg aggaggaagg cactgttgag ttatctgccg gcggcggagg gagtggagga
     2581 gggggaagcg gaggcggagg gagtagaatt ctgcagtcga cggtaccgcg ggcccgggat
     2641 ccaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     2701 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     2761 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     2821 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
     2881 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     2941 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     3001 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     3061 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
     3121 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
     3181 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     3241 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
     3301 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     3361 gagctgtaca agtaaagcgg ccgcgactct agatcataat cagccatacc acatttgtag
     3421 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     3481 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     3541 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     3601 aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta atattttgtt aaaattcgcg
     3661 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
     3721 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     3781 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
     3841 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
     3901 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
     3961 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
     4021 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
     4081 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     4141 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     4201 aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg
     4261 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     4321 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     4381 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     4441 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     4501 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     4561 gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
     4621 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     4681 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     4741 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg
     4801 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     4861 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     4921 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     4981 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     5041 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     5101 gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc
     5161 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     5221 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     5281 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     5341 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     5401 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
     5461 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     5521 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga
     5581 ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa
     5641 aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc
     5701 agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt
     5761 cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg
     5821 tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag attgatttaa
     5881 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     5941 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     6001 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     6061 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     6121 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     6181 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     6241 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     6301 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     6361 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     6421 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     6481 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     6541 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     6601 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     6661 ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat
//
