LOCUS       V042242                 6729 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V042242
VERSION     V042242
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             613..1329
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="enhanced GFP with monomerizing A206K mutation
                     (Zacharias et al., 2002)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="mEGFP"
     CDS             1399..3384
                     /label="DDX3X(NM_001356)"
                     /note="DDX3X(NM_001356)"
                     /gene="DDX3X"
     polyA_signal    3517..3638
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3645..4100)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4127..4231
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        4233..4590
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4625..5419
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5651..5698
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      6027..6615
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc aagctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgaggt ggaggtggat caggtggtgg aggatctaag
     1381 cttctgcgtc gagaattcat gagtcatgtg gcagtggaaa atgcgctcgg gctggaccag
     1441 cagtttgctg gcctagacct gaactcttca gataatcaga gtggaggaag tacagccagc
     1501 aaagggcgct atattcctcc tcatttaagg aaccgagaag ctactaaagg tttctacgat
     1561 aaagacagtt cagggtggag ttctagcaaa gataaggatg cgtatagcag ttttggatct
     1621 cgtagtgatt caagagggaa gtctagcttc ttcagtgatc gtggaagtgg atcaagggga
     1681 aggtttgatg atcgtggacg gagtgattac gatggcattg gcagccgtgg tgacagaagt
     1741 ggctttggca aatttgaacg tggtggaaac agtcgctggt gtgacaaatc agatgaagat
     1801 gattggtcaa aaccactccc accaagtgaa cgcttggaac aggaactctt ttctggaggc
     1861 aacactggga ttaattttga gaaatacgat gacattccag ttgaggcaac aggcaacaac
     1921 tgtcctccac atattgaaag tttcagtgat gttgagatgg gagaaattat catgggaaac
     1981 attgagctta ctcgttatac tcgcccaact ccagtgcaaa agcatgctat tcctattatc
     2041 aaagagaaaa gagacttgat ggcttgtgcc caaacagggt ctggaaaaac tgcagcattt
     2101 ctgttgccca tcttgagtca gatttattca gatggtccag gcgaggcttt gagggccatg
     2161 aaggaaaatg gaaggtatgg gcgccgcaaa caatacccaa tctccttggt attagcacca
     2221 acgagagagt tggcagtaca gatctacgag gaagccagaa aattttcata ccgatctaga
     2281 gttcgtcctt gcgtggttta tggtggtgcc gatattggtc agcagattcg agacttggaa
     2341 cgtggatgcc atttgttagt agccactcca ggacgtctag tggatatgat ggaaagagga
     2401 aagattggat tagacttttg caaatacttg gtgttagatg aagctgatcg gatgttggat
     2461 atggggtttg agcctcagat tcgtagaata gtcgaacaag atactatgcc tccaaagggt
     2521 gtccgccaca ctatgatgtt tagtgctact tttcctaagg aaatacagat gctggctcgt
     2581 gatttcttag atgaatatat cttcttggct gtaggaagag ttggctctac ctctgaaaac
     2641 atcacacaga aagtagtttg ggtggaagaa tcagacaaac ggtcatttct gcttgacctc
     2701 ctaaatgcaa caggcaagga ttcactgacc ttagtgtttg tggagaccaa aaagggtgca
     2761 gattctctgg aggatttctt ataccatgaa ggatacgcat gtaccagcat ccatggagac
     2821 cgttctcaga gggatagaga agaggccctt caccagttcc gctcaggaaa aagcccaatt
     2881 ttagtggcta cagcagtagc agcaagagga ctggacattt caaatgtgaa acatgttatc
     2941 aattttgact tgccaagtga tattgaagaa tatgtacatc gtattggtcg tacgggacgt
     3001 gtaggaaacc ttggcctggc aacctcattc tttaacgaga ggaacataaa tattactaag
     3061 gatttgttgg atcttcttgt tgaagctaaa caagaagtgc cgtcttggtt agaaaacatg
     3121 gcttatgaac accactacaa gggtagcagt cgtggacgtt ctaagagtag cagatttagt
     3181 ggagggtttg gtgccagaga ctaccgacaa agtagcggtg ccagcagttc cagcttcagc
     3241 agcagccgcg caagcagcag ccgcagtggc ggaggtggcc acggtagcag cagaggattt
     3301 ggtggaggtg gctatggagg cttttacaac agtgatggat atggaggaaa ttataactcc
     3361 cagggggttg actggtgggg taactgagga tccaccggat ctagataact gatcataatc
     3421 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
     3481 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
     3541 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     3601 tctagttgtg gtttgtccaa actcatcaat gtatcttaac gcgtaaattg taagcgttaa
     3661 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
     3721 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
     3781 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
     3841 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
     3901 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     3961 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     4021 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
     4081 tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     4141 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     4201 aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat
     4261 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     4321 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     4381 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     4441 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     4501 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     4561 ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt
     4621 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     4681 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     4741 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     4801 actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     4861 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     4921 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     4981 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     5041 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     5101 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc
     5161 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     5221 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     5281 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     5341 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     5401 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc
     5461 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     5521 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     5581 ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc
     5641 cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat
     5701 aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg
     5761 ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc
     5821 ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat
     5881 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     5941 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     6001 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     6061 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     6121 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     6181 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     6241 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     6301 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     6361 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     6421 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     6481 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     6541 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     6601 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     6661 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     6721 gccatgcat
//
