LOCUS       V042404                 6695 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V042404
VERSION     V042404
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             641..1294
                     /codon_start=1
                     /gene=""
                     /product="glutathione S-transferase from Schistosoma
                     japonicum"
                     /transl_table=1
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
                     /label="GST"
     CDS             1331..3316
                     /label="DDX3X(NM_001356)"
                     /note="DDX3X(NM_001356)"
                     /gene="DDX3X"
     CDS             3323..3349
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     polyA_signal    3483..3604
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3611..4066)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4093..4197
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        4199..4556
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4591..5385
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5617..5664
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5993..6581
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attcgccacc atgtccccta tactaggtta
      661 ttggaaaatt aagggccttg tgcaacccac tcgacttctt ttggaatatc ttgaagaaaa
      721 atatgaagag catttgtatg agcgcgatga aggtgataaa tggcgaaaca aaaagtttga
      781 attgggtttg gagtttccca atcttcctta ttatattgat ggtgatgtta aattaacaca
      841 gtctatggcc atcatacgtt atatagctga caagcacaac atgttgggtg gttgtccaaa
      901 agagcgtgca gagatttcaa tgcttgaagg agcggttttg gatattagat acggtgtttc
      961 gagaattgca tatagtaaag actttgaaac tctcaaagtt gattttctta gcaagctacc
     1021 tgaaatgctg aaaatgttcg aagatcgttt atgtcataaa acatatttaa atggtgatca
     1081 tgtaacccat cctgacttca tgttgtatga cgctcttgat gttgttttat acatggaccc
     1141 aatgtgcctg gatgcgttcc caaaattagt ttgttttaaa aaacgtattg aagctatccc
     1201 acaaattgat aagtacttga aatccagcaa gtatatagca tggcctttgc agggctggca
     1261 agccacgttt ggtggtggcg accatcctcc aaaaggagga gggggaagcg gcggcggagg
     1321 gagtaagctt atgagtcatg tggcagtgga aaatgcgctc gggctggacc agcagtttgc
     1381 tggcctagac ctgaactctt cagataatca gagtggagga agtacagcca gcaaagggcg
     1441 ctatattcct cctcatttaa ggaaccgaga agctactaaa ggtttctacg ataaagacag
     1501 ttcagggtgg agttctagca aagataagga tgcgtatagc agttttggat ctcgtagtga
     1561 ttcaagaggg aagtctagct tcttcagtga tcgtggaagt ggatcaaggg gaaggtttga
     1621 tgatcgtgga cggagtgatt acgatggcat tggcagccgt ggtgacagaa gtggctttgg
     1681 caaatttgaa cgtggtggaa acagtcgctg gtgtgacaaa tcagatgaag atgattggtc
     1741 aaaaccactc ccaccaagtg aacgcttgga acaggaactc ttttctggag gcaacactgg
     1801 gattaatttt gagaaatacg atgacattcc agttgaggca acaggcaaca actgtcctcc
     1861 acatattgaa agtttcagtg atgttgagat gggagaaatt atcatgggaa acattgagct
     1921 tactcgttat actcgcccaa ctccagtgca aaagcatgct attcctatta tcaaagagaa
     1981 aagagacttg atggcttgtg cccaaacagg gtctggaaaa actgcagcat ttctgttgcc
     2041 catcttgagt cagatttatt cagatggtcc aggcgaggct ttgagggcca tgaaggaaaa
     2101 tggaaggtat gggcgccgca aacaataccc aatctccttg gtattagcac caacgagaga
     2161 gttggcagta cagatctacg aggaagccag aaaattttca taccgatcta gagttcgtcc
     2221 ttgcgtggtt tatggtggtg ccgatattgg tcagcagatt cgagacttgg aacgtggatg
     2281 ccatttgtta gtagccactc caggacgtct agtggatatg atggaaagag gaaagattgg
     2341 attagacttt tgcaaatact tggtgttaga tgaagctgat cggatgttgg atatggggtt
     2401 tgagcctcag attcgtagaa tagtcgaaca agatactatg cctccaaagg gtgtccgcca
     2461 cactatgatg tttagtgcta cttttcctaa ggaaatacag atgctggctc gtgatttctt
     2521 agatgaatat atcttcttgg ctgtaggaag agttggctct acctctgaaa acatcacaca
     2581 gaaagtagtt tgggtggaag aatcagacaa acggtcattt ctgcttgacc tcctaaatgc
     2641 aacaggcaag gattcactga ccttagtgtt tgtggagacc aaaaagggtg cagattctct
     2701 ggaggatttc ttataccatg aaggatacgc atgtaccagc atccatggag accgttctca
     2761 gagggataga gaagaggccc ttcaccagtt ccgctcagga aaaagcccaa ttttagtggc
     2821 tacagcagta gcagcaagag gactggacat ttcaaatgtg aaacatgtta tcaattttga
     2881 cttgccaagt gatattgaag aatatgtaca tcgtattggt cgtacgggac gtgtaggaaa
     2941 ccttggcctg gcaacctcat tctttaacga gaggaacata aatattacta aggatttgtt
     3001 ggatcttctt gttgaagcta aacaagaagt gccgtcttgg ttagaaaaca tggcttatga
     3061 acaccactac aagggtagca gtcgtggacg ttctaagagt agcagattta gtggagggtt
     3121 tggtgccaga gactaccgac aaagtagcgg tgccagcagt tccagcttca gcagcagccg
     3181 cgcaagcagc agccgcagtg gcggaggtgg ccacggtagc agcagaggat ttggtggagg
     3241 tggctatgga ggcttttaca acagtgatgg atatggagga aattataact cccagggggt
     3301 tgactggtgg ggtaacctcg agtacccata cgacgtacct gattatgcat agggcccggg
     3361 atcggccgcg actctagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
     3421 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
     3481 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     3541 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     3601 cttaaggcgt aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     3661 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
     3721 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     3781 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
     3841 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
     3901 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     3961 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
     4021 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt
     4081 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     4141 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtcct
     4201 gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     4261 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     4321 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     4381 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     4441 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     4501 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaagatc
     4561 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
     4621 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     4681 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     4741 accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg
     4801 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     4861 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     4921 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     4981 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     5041 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     5101 ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     5161 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     5221 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     5281 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     5341 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
     5401 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg
     5461 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     5521 agcgcgggga tctcatgctg gagttcttcg cccaccctag ggggaggcta actgaaacac
     5581 ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa
     5641 cgcacggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg
     5701 tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac
     5761 cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc
     5821 ctgccatagc ctcaggttac tcatatatac tttagattga tttaaaactt catttttaat
     5881 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     5941 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     6001 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     6061 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     6121 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     6181 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     6241 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     6301 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     6361 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     6421 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     6481 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     6541 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     6601 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     6661 ctgattctgt ggataaccgt attaccgcca tgcat
//
