LOCUS V026471 5577 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V026471 VERSION V026471 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 61..364 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 365..568 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 613..1329 /codon_start=1 /note="mammalian codon-optimized" /product="enhanced CFP" /transl_table=1 /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNG IKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV LLEFVTAAGITLGMDELYK" /label="ECFP" CDS 1363..2232 /label="CD274(NM_014143)" /note="CD274(NM_014143)" /gene="CD274" polyA_signal 2365..2486 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(2493..2948) /direction=LEFT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2975..3079 /gene="bla" /label="AmpR promoter" promoter 3081..3438 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3473..4267 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4499..4546 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 4875..5463 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg 661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc 721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg 781 ccctggccca ccctcgtgac caccctgacc tggggcgtgc agtgcttcag ccgctacccc 841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag 901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag 961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac 1021 atcctggggc acaagctgga gtacaactac atcagccaca acgtctatat caccgccgac 1081 aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcagc 1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg 1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc 1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag 1321 ctgtacaagt ccggactcag atctcgagga ggtggaggtt caatgaggat atttgctgtc 1381 tttatattca tgacctactg gcatttgctg aacgcattta ctgtcacggt tcccaaggac 1441 ctatatgtgg tagagtatgg tagcaatatg acaattgaat gcaaattccc agtagaaaaa 1501 caattagacc tggctgcact aattgtctat tgggaaatgg aggataagaa cattattcaa 1561 tttgtgcatg gagaggaaga cctgaaggtt cagcatagta gctacagaca gagggcccgg 1621 ctgttgaagg accagctctc cctgggaaat gctgcacttc agatcacaga tgtgaaattg 1681 caggatgcag gggtgtaccg ctgcatgatc agctatggtg gtgccgacta caagcgaatt 1741 actgtgaaag tcaatgcccc atacaacaaa atcaaccaaa gaattttggt tgtggatcca 1801 gtcacctctg aacatgaact gacatgtcag gctgagggct accccaaggc cgaagtcatc 1861 tggacaagca gtgaccatca agtcctgagt ggtaagacca ccaccaccaa ttccaagaga 1921 gaggagaagc ttttcaatgt gaccagcaca ctgagaatca acacaacaac taatgagatt 1981 ttctactgca cttttaggag attagatcct gaggaaaacc atacagctga attggtcatc 2041 ccagaactac ctctggcaca tcctccaaat gaaaggactc acttggtaat tctgggagcc 2101 atcttattat gccttggtgt agcactgaca ttcatcttcc gtttaagaaa agggagaatg 2161 atggatgtga aaaaatgtgg catccaagat acaaactcaa agaagcaaag tgatacacat 2221 ttggaggaga cgtaaggatc caccggatct agataactga tcataatcag ccataccaca 2281 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat 2341 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa 2401 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt 2461 ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta agcgttaata ttttgttaaa 2521 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa 2581 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa 2641 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca 2701 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg 2761 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc 2821 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc 2881 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca 2941 gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt 3001 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata 3061 atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta 3121 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat 3181 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc 3241 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta 3301 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca 3361 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga 3421 ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga 3481 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga 3541 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg 3601 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga 3661 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt 3721 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct 3781 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct 3841 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg 3901 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca 3961 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga 4021 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt 4081 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt 4141 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct 4201 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt 4261 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca 4321 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg 4381 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct 4441 agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg 4501 gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt 4561 cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc 4621 cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca 4681 gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat actttagatt 4741 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc 4801 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag 4861 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa 4921 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg 4981 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag 5041 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg 5101 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga 5161 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc 5221 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc 5281 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga 5341 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt 5401 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg 5461 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac 5521 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc catgcat //