LOCUS       V035604                 6670 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V035604
VERSION     V035604
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             613..1320
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             1357..3294
                     /label="CANX(NM_001363993)"
                     /note="CANX(NM_001363993)"
                     /gene="CANX"
     polyA_signal    3458..3579
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3586..4041)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4068..4172
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        4174..4531
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4566..5360
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5592..5639
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5968..6556
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
      661 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
      721 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
      781 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
      841 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
      901 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
      961 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     1021 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     1081 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     1141 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     1201 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     1261 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     1321 ggtggaggcg gatcaggagg cggtggatct ctcgagatgt accagaagga gaacacgcag
     1381 tggttgagaa acttagcagt tacagcacct attctggagg cgagtaatcc agttggagag
     1441 ggacagggcg ttaaaagcag gaaagccccc gaaagctcat ctaagtgcat ctctttccac
     1501 accggaggcc tggagatcat ggaagggaag tggttgctgt gtatgttact ggtgcttgga
     1561 actgctattg ttgaggctca tgatggacat gatgatgatg tgattgatat tgaggatgac
     1621 cttgacgatg tcattgaaga ggtagaagac tcaaaaccag ataccactgc tcctccttca
     1681 tctcccaagg ttacttacaa agctccagtt ccaacagggg aagtatattt tgctgattct
     1741 tttgacagag gaactctgtc agggtggatt ttatccaaag ccaagaaaga cgataccgat
     1801 gatgaaattg ccaaatatga tggaaagtgg gaggtagagg aaatgaagga gtcaaagctt
     1861 ccaggtgata aaggacttgt gttgatgtct cgggccaagc atcatgccat ctctgctaaa
     1921 ctgaacaagc ccttcctgtt tgacaccaag cctctcattg ttcagtatga ggttaatttc
     1981 caaaatggaa tagaatgtgg tggtgcctat gtgaaactgc tttctaaaac accagaactc
     2041 aacctggatc agttccatga caagacccct tatacgatta tgtttggtcc agataaatgt
     2101 ggagaggact ataaactgca cttcatcttc cgacacaaaa accccaaaac gggtatctat
     2161 gaagaaaaac atgctaagag gccagatgca gatctgaaga cctattttac tgataagaaa
     2221 acacatcttt acacactaat cttgaatcca gataatagtt ttgaaatact ggttgaccaa
     2281 tctgtggtga atagtggaaa tctgctcaat gacatgactc ctcctgtaaa tccttcacgt
     2341 gaaattgagg acccagaaga ccggaagccc gaggattggg atgaaagacc aaaaatccca
     2401 gatccagaag ctgtcaagcc agatgactgg gatgaagatg cccctgctaa gattccagat
     2461 gaagaggcca caaaacccga aggctggtta gatgatgagc ctgagtacgt acctgatcca
     2521 gacgcagaga aacctgagga ttgggatgaa gacatggatg gagaatggga ggctcctcag
     2581 attgccaacc ctagatgtga gtcagctcct ggatgtggtg tctggcagcg acctgtgatt
     2641 gacaacccca attataaagg caaatggaag cctcctatga ttgacaatcc cagttaccag
     2701 ggaatctgga aacccaggaa aataccaaat ccagatttct ttgaagatct ggaacctttc
     2761 agaatgactc cttttagtgc tattggtttg gagctgtggt ccatgacctc tgacattttt
     2821 tttgacaact ttatcatttg tgctgatcga agaatagttg atgattgggc caatgatgga
     2881 tggggcctga agaaagctgc tgatggggct gctgagccag gcgttgtggg gcagatgatc
     2941 gaggcagctg aagagcgccc gtggctgtgg gtagtctata ttctaactgt agcccttcct
     3001 gtgttcctgg ttatcctctt ctgctgttct ggaaagaaac agaccagtgg tatggagtat
     3061 aagaaaactg atgcacctca accggatgtg aaggaagagg aagaagagaa ggaagaggaa
     3121 aaggacaagg gagatgagga ggaggaagga gaagagaaac ttgaagagaa acagaaaagt
     3181 gatgctgaag aagatggtgg cactgtcagt caagaggagg aagacagaaa acctaaagca
     3241 gaggaggatg aaattttgaa cagatcacca agaaacagaa agccacgaag agagtgagaa
     3301 ttctgcagtc gacggtaccg cgggcccggg atccaccgga tctagataac tgatcataat
     3361 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     3421 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
     3481 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     3541 ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa cgcgtaaatt gtaagcgtta
     3601 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     3661 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     3721 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     3781 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
     3841 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     3901 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     3961 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     4021 atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     4081 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     4141 aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa
     4201 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     4261 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     4321 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     4381 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     4441 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     4501 aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt
     4561 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     4621 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     4681 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     4741 aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     4801 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     4861 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     4921 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     4981 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     5041 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc
     5101 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     5161 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     5221 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     5281 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     5341 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
     5401 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     5461 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     5521 cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac
     5581 ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca
     5641 taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg
     5701 gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg
     5761 cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata
     5821 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     5881 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     5941 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     6001 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     6061 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     6121 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     6181 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     6241 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     6301 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     6361 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     6421 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     6481 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     6541 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     6601 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     6661 cgccatgcat
//
