LOCUS V033627 6477 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V033627 VERSION V033627 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 61..364 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 365..568 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 603..1301 /codon_start=1 /note="mammalian codon-optimized" /product="monomeric blue fluorescent protein" /transl_table=1 /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC DLPSKLGHKLN" /label="TagBFP" CDS 1353..3128 /label="CANX(NM_001746)" /note="CANX(NM_001746)" /gene="CANX" polyA_signal 3265..3386 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(3393..3848) /direction=LEFT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3875..3979 /gene="bla" /label="AmpR promoter" promoter 3981..4338 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4373..5167 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5399..5446 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 5775..6363 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcca 601 ccatgagcga gctgattaag gagaacatgc acatgaagct gtacatggag ggcaccgtgg 661 acaaccatca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag ggcacccaga 721 ccatgagaat caaggtggtc gagggcggcc ctctcccctt cgccttcgac atcctggcta 781 ctagcttcct ctacggcagc aagaccttca tcaaccacac ccagggcatc cccgacttct 841 tcaagcagtc cttccctgag ggcttcacat gggagagagt caccacatac gaagacgggg 901 gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc tacaacgtca 961 agatcagagg ggtgaacttc acatccaacg gccctgtgat gcagaagaaa acactcggct 1021 gggaggcctt caccgagacg ctgtaccccg ctgacggcgg cctggaaggc agaaacgaca 1081 tggccctgaa gctcgtgggc gggagccatc tgatcgcaaa catcaagacc acatatagat 1141 ccaagaaacc cgctaagaac ctcaagatgc ctggcgtcta ctatgtggac tacagactgg 1201 aaagaatcaa ggaggccaac aacgagacct acgtcgagca gcacgaggtg gcagtggcca 1261 gatactgcga cctccctagc aaactggggc acaagcttaa tggcggcgga gggagtggag 1321 gagggggaag cggaggcgga gggagtctcg agatggaagg gaagtggttg ctgtgtatgt 1381 tactggtgct tggaactgct attgttgagg ctcatgatgg acatgatgat gatgtgattg 1441 atattgagga tgaccttgac gatgtcattg aagaggtaga agactcaaaa ccagatacca 1501 ctgctcctcc ttcatctccc aaggttactt acaaagctcc agttccaaca ggggaagtat 1561 attttgctga ttcttttgac agaggaactc tgtcagggtg gattttatcc aaagccaaga 1621 aagacgatac cgatgatgaa attgccaaat atgatggaaa gtgggaggta gaggaaatga 1681 aggagtcaaa gcttccaggt gataaaggac ttgtgttgat gtctcgggcc aagcatcatg 1741 ccatctctgc taaactgaac aagcccttcc tgtttgacac caagcctctc attgttcagt 1801 atgaggttaa tttccaaaat ggaatagaat gtggtggtgc ctatgtgaaa ctgctttcta 1861 aaacaccaga actcaacctg gatcagttcc atgacaagac cccttatacg attatgtttg 1921 gtccagataa atgtggagag gactataaac tgcacttcat cttccgacac aaaaacccca 1981 aaacgggtat ctatgaagaa aaacatgcta agaggccaga tgcagatctg aagacctatt 2041 ttactgataa gaaaacacat ctttacacac taatcttgaa tccagataat agttttgaaa 2101 tactggttga ccaatctgtg gtgaatagtg gaaatctgct caatgacatg actcctcctg 2161 taaatccttc acgtgaaatt gaggacccag aagaccggaa gcccgaggat tgggatgaaa 2221 gaccaaaaat cccagatcca gaagctgtca agccagatga ctgggatgaa gatgcccctg 2281 ctaagattcc agatgaagag gccacaaaac ccgaaggctg gttagatgat gagcctgagt 2341 acgtacctga tccagacgca gagaaacctg aggattggga tgaagacatg gatggagaat 2401 gggaggctcc tcagattgcc aaccctagat gtgagtcagc tcctggatgt ggtgtctggc 2461 agcgacctgt gattgacaac cccaattata aaggcaaatg gaagcctcct atgattgaca 2521 atcccagtta ccagggaatc tggaaaccca ggaaaatacc aaatccagat ttctttgaag 2581 atctggaacc tttcagaatg actcctttta gtgctattgg tttggagctg tggtccatga 2641 cctctgacat tttttttgac aactttatca tttgtgctga tcgaagaata gttgatgatt 2701 gggccaatga tggatggggc ctgaagaaag ctgctgatgg ggctgctgag ccaggcgttg 2761 tggggcagat gatcgaggca gctgaagagc gcccgtggct gtgggtagtc tatattctaa 2821 ctgtagccct tcctgtgttc ctggttatcc tcttctgctg ttctggaaag aaacagacca 2881 gtggtatgga gtataagaaa actgatgcac ctcaaccgga tgtgaaggaa gaggaagaag 2941 agaaggaaga ggaaaaggac aagggagatg aggaggagga aggagaagag aaacttgaag 3001 agaaacagaa aagtgatgct gaagaagatg gtggcactgt cagtcaagag gaggaagaca 3061 gaaaacctaa agcagaggag gatgaaattt tgaacagatc accaagaaac agaaagccac 3121 gaagagagtg aggatccacc ggtgcggccg cgactctaga tcataatcag ccataccaca 3181 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat 3241 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa 3301 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt 3361 ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta agcgttaata ttttgttaaa 3421 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa 3481 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa 3541 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca 3601 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg 3661 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc 3721 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc 3781 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca 3841 gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt 3901 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata 3961 atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta 4021 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat 4081 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc 4141 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta 4201 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca 4261 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga 4321 ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga 4381 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga 4441 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg 4501 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga 4561 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt 4621 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct 4681 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct 4741 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg 4801 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca 4861 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga 4921 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt 4981 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt 5041 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct 5101 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt 5161 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca 5221 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg 5281 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct 5341 agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg 5401 gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt 5461 cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc 5521 cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca 5581 gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat actttagatt 5641 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc 5701 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag 5761 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa 5821 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg 5881 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag 5941 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg 6001 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga 6061 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc 6121 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc 6181 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga 6241 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt 6301 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg 6361 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac 6421 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc catgcat //