LOCUS       V037723                 7328 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V037723
VERSION     V037723
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     regulatory      595..604
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             604..669
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     CDS             670..3972
                     /label="Acly(NM_001199296)"
                     /note="Acly(NM_001199296)"
                     /gene="Acly"
     polyA_signal    4116..4237
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(4244..4699)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4726..4830
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        4832..5189
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5224..6018
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6250..6297
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      6626..7214
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagccacc
      601 atggactaca aagaccatga cggtgattat aaagatcatg acatcgacta caaggatgac
      661 gatgacaaga tgtcagccaa ggcaatttca gagcagaccg gcaaagaact cctgtacaag
      721 tacatctgca ccacctcggc catccagaac cggttcaagt acgcccgggt gactcccgac
      781 acagactggg cccatctgct gcaggaccac ccgtggctgc tcagccagag cttggttgtc
      841 aagccagacc agttaatcaa acgtcgagga aagcttggcc tcgtcggggt caatctctct
      901 ctggatggag tcaaatcctg gctaaaacct cgcctgggac atgaagccac tgtcggcaag
      961 gccaaaggct tcctcaagaa ctttctcatt gaacccttcg tcccccacag tcaggcggag
     1021 gagttctacg tgtgcatcta tgctacccgg gaaggagact acgtcctgtt ccaccatgaa
     1081 gggggtgtgg atgtgggcga tgtggatgcc aaagcccaga agctgcttgt gggtgtggac
     1141 gaaaagctga ataccgagga cattaagaga cacctgttgg tccatgcacc tgaggacaag
     1201 aaagaagtcc tggccagctt catctctggt ctattcaatt tctacgagga tctgtacttc
     1261 acctaccttg agatcaaccc ccttgtggtg accaaagatg gtgtctacat ccttgacttg
     1321 gcggccaagg tggatgccac agctgactac atctgtaaag tcaagtgggg tgatatagag
     1381 ttccctcccc cctttgggcg tgaggcgtac cccgaggaag cctacattgc agacctggat
     1441 gccaaaagtg gagcaagctt gaagctgacc ttgctgaacc ccaaggggcg gatctggacc
     1501 atggttgctg ggggtggcgc ctccgtcgtg tacagtgaca ccatctgtga tcttggaggt
     1561 gtcaatgaac tggcgaatta cggggaatac tcgggtgccc ccagtgaaca acagacctat
     1621 gactatgcca agaccatcct ctcacttatg actcgagaga agcacccaga aggcaagatc
     1681 ctcatcattg gaggcagcat tgcaaacttc accaatgtgg ccgccacctt caagggcatt
     1741 gtgagagcga ttcgagatta ccagggtccc ctgaaggagc atgaggtcac catctttgtc
     1801 cgaagaggtg gccccaacta tcaagaggga ttacgagtga tgggagaagt tgggaagacc
     1861 actgggatcc ccatccatgt ctttggcaca gaaactcaca tgacggccat tgtgggcatg
     1921 gccctgggcc accggcccat tcccaaccag ccacccacag cagctcacac tgccaacttc
     1981 ctccttaatg ccagcgggag cacatcgact ccagcaccca gtaggacagc atctttttct
     2041 gagtcccgag ctgatgaagt ggcacctgca aagaaagcca agccagctat gccccaagat
     2101 tcagtcccaa gtccaagatc cctgcaagga aagagtgcca ccctcttcag ccgacatacc
     2161 aaggccatcg tgtggggcat gcagacccgg gctgtgcagg gcatgctgga ctttgactac
     2221 gtgtgttccc gagacgagcc ctcagtggct gccatggtct accctttcac tggggatcac
     2281 aagcagaagt tttactgggg acacaaggaa atcctgatcc ctgtcttcaa gaacatggct
     2341 gacgccatga agaagcaccc ggaggtagac gtgctgatca actttgcgtc tctgcggtcc
     2401 gcttacgaca gcaccatgga gaccatgaac tatgcccaga tccgcaccat agccatcata
     2461 gcagaaggta tccctgaggc cctcacacgg aagctcatca agaaggccga ccagaaggga
     2521 gtgaccatca ttgggccagc tacggttggg ggcattaagc ctggatgctt taagatcggg
     2581 aatactggtg gaatgctgga caacatcctg gcctccaaac tgtaccgccc aggcagcgtg
     2641 gcctacgtct cacgttcagg aggcatgtct aatgaactca ataacatcat ctctcggacc
     2701 acagatggtg tctatgaggg cgtggccatc ggcggggaca ggtaccctgg gtccacattc
     2761 atggatcacg tgctgcgcta ccaggacact ccaggagtca agatgatcgt agttcttggg
     2821 gagatagggg gcacagagga atataagatc tgccggggca tcaaggaggg ccgcctcacc
     2881 aagccagtgg tctgctggtg tatcgggacc tgtgccacca tgttctcctc cgaggtccag
     2941 tttggccatg ctggagcttg tgccaaccag gcttctgaaa ctgcagtagc caagaaccag
     3001 gccttgaagg aagcaggagt gtttgtgccc cgaagcttcg atgagcttgg agaaatcatt
     3061 cagtctgtgt atgaagatct ggtggccaaa ggagccattg tacctgccca ggaagtgcca
     3121 cctccaacag tgcccatgga ctactcttgg gccagagagc tgggtttgat ccgaaaacct
     3181 gcctcattca tgaccagcat ctgtgatgag cgagggcagg agctcattta tgcgggcatg
     3241 cccatcaccg aggtcttcaa ggaggagatg ggcatcggtg gtgtcctcgg cctcctctgg
     3301 ttccagagaa ggttgcccaa gtattcctgc cagttcattg agatgtgtct gatggtcaca
     3361 gctgatcacg ggccagctgt ctctggagcc cataacacca tcatctgtgc tcgggctggg
     3421 aaggacctgg tctccagcct cacctcaggg ctgctcacca ttggagaccg gtttgggggt
     3481 gccttggatg ccgcagcaaa gatgttcagt aaagcctttg acagcggcat cattcccatg
     3541 gagttcgtca acaagatgaa gaaggagggg aagctgatca tgggcatcgg ccatcgagta
     3601 aaatcgataa acaacccaga catgcgagtg cagatcctca aggacttcgt caaacagcac
     3661 ttccccgcca ccccgctgct cgactatgcc ctggaagtgg agaagattac cacctccaag
     3721 aagccaaatc ttatcctgaa tgtggacggc ttcatcggcg ttgcgtttgt ggacatgctc
     3781 aggaactgtg gctccttcac ccgggaggaa gctgatgaat atgttgacat tggagccctc
     3841 aatggcatct ttgtgctagg aaggagtatg ggcttcattg ggcactacct tgaccagaag
     3901 aggctgaagc aagggctgta tcgtcacccc tgggatgaca tttcctatgt tcttccagaa
     3961 cacatgagca tgtaaccgcg ggcccgggat ccaccggatc tagataactg atcataatca
     4021 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     4081 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     4141 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     4201 ctagttgtgg tttgtccaaa ctcatcaatg tatcttaacg cgtaaattgt aagcgttaat
     4261 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
     4321 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
     4381 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
     4441 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
     4501 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
     4561 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
     4621 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
     4681 gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     4741 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     4801 atgcttcaat aatattgaaa aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg
     4861 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     4921 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     4981 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     5041 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     5101 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     5161 gcttttttgg aggcctaggc ttttgcaaag atcgatcaag agacaggatg aggatcgttt
     5221 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
     5281 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     5341 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     5401 ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     5461 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     5521 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     5581 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     5641 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     5701 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc
     5761 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
     5821 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     5881 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     5941 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     6001 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca
     6061 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     6121 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     6181 tcgcccaccc tagggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc
     6241 gcgctatgac ggcaataaaa agacagaata aaacgcacgg tgttgggtcg tttgttcata
     6301 aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg
     6361 gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc
     6421 cagggctcgc agccaacgtc ggggcggcag gccctgccat agcctcaggt tactcatata
     6481 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     6541 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     6601 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     6661 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     6721 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     6781 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     6841 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     6901 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     6961 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     7021 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     7081 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     7141 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     7201 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     7261 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     7321 ccatgcat
//
