LOCUS       V021466                 5731 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V021466
VERSION     V021466
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             627..653
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             654..1643
                     /label="ARG1(NM_001244438)"
                     /note="ARG1(NM_001244438)"
                     /gene="ARG1"
     CDS             1677..2396
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     polyA_signal    2519..2640
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(2647..3102)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3129..3233
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        3235..3592
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             3627..4421
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    4653..4700
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5029..5617
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagcc accatgtacc catacgacgt accagattac gctatgagcg
      661 ccaagtccag aaccataggg attattggag ctcctttctc aaagggacag ccacgaggag
      721 gggtggaaga aggccctaca gtattgagaa aggctggtct gcttgagaaa cttaaagaac
      781 aagtaactca aaacttttta attttagagt gtgatgtgaa ggattatggg gacctgccct
      841 ttgctgacat ccctaatgac agtccctttc aaattgtgaa gaatccaagg tctgtgggaa
      901 aagcaagcga gcagctggct ggcaaggtgg cagaagtcaa gaagaacgga agaatcagcc
      961 tggtgctggg cggagaccac agtttggcaa ttggaagcat ctctggccat gccagggtcc
     1021 accctgatct tggagtcatc tgggtggatg ctcacactga tatcaacact ccactgacaa
     1081 ccacaagtgg aaacttgcat ggacaacctg tatctttcct cctgaaggaa ctaaaaggaa
     1141 agattcccga tgtgccagga ttctcctggg tgactccctg tatatctgcc aaggatattg
     1201 tgtatattgg cttgagagac gtggaccctg gggaacacta cattttgaaa actctaggca
     1261 ttaaatactt ttcaatgact gaagtggaca gactaggaat tggcaaggtg atggaagaaa
     1321 cactcagcta tctactagga agaaagaaaa ggccaattca tctaagtttt gatgttgacg
     1381 gactggaccc atctttcaca ccagctactg gcacaccagt cgtgggaggt ctgacataca
     1441 gagaaggtct ctacatcaca gaagaaatct acaaaacagg gctactctca ggattagata
     1501 taatggaagt gaacccatcc ctggggaaga caccagaaga agtaactcga acagtgaaca
     1561 cagcagttgc aataaccttg gcttgtttcg gacttgctcg ggagggtaat cacaagccta
     1621 ttgactacct taacccacct aagggaggag ggggaagcga tccaccggtc gccaccatgg
     1681 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     1741 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     1801 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     1861 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     1921 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     1981 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     2041 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     2101 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     2161 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     2221 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     2281 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     2341 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaagcg
     2401 gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     2461 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     2521 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     2581 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     2641 aggcgtaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     2701 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     2761 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     2821 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     2881 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     2941 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     3001 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     3061 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg
     3121 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     3181 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg
     3241 cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     3301 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     3361 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     3421 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     3481 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     3541 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc
     3601 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc
     3661 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     3721 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     3781 acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca
     3841 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     3901 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     3961 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     4021 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     4081 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     4141 ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
     4201 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     4261 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     4321 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     4381 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga
     4441 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt
     4501 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     4561 cggggatctc atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa
     4621 ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca
     4681 cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga
     4741 taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca
     4801 ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc
     4861 catagcctca ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     4921 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     4981 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     5041 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     5101 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     5161 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     5221 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     5281 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     5341 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     5401 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     5461 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     5521 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     5581 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     5641 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     5701 ttctgtggat aaccgtatta ccgccatgca t
//
