LOCUS       V018797                 6095 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V018797
VERSION     V018797
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             625..2019
                     /label="ADRA2A(NM_000681)"
                     /note="ADRA2A(NM_000681)"
                     /gene="ADRA2A"
     CDS             2041..2760
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     polyA_signal    2883..3004
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3011..3466)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3493..3597
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        3599..3956
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             3991..4785
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5017..5064
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5393..5981
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgaggc caccatgttc cgccaggagc agccgttggc cgagggcagc
      661 tttgcgccca tgggctccct gcagccggac gcgggcaacg cgagctggaa cgggaccgag
      721 gcgccggggg gcggcgcccg ggccacccct tactccctgc aggtgacgct gacgctggtg
      781 tgcctggccg gcctgctcat gctgctcacc gtgttcggca acgtgctcgt catcatcgcc
      841 gtgttcacga gccgcgcgct caaggcgccc caaaacctct tcctggtgtc tctggcctcg
      901 gccgacatcc tggtggccac gctcgtcatc cctttctcgc tggccaacga ggtcatgggc
      961 tactggtact tcggcaaggc ttggtgcgag atctacctgg cgctcgacgt gctcttctgc
     1021 acgtcgtcca tcgtgcacct gtgcgccatc agcctggacc gctactggtc catcacacag
     1081 gccatcgagt acaacctgaa gcgcacgccg cgccgcatca aggccatcat catcaccgtg
     1141 tgggtcatct cggccgtcat ctccttcccg ccgctcatct ccatcgagaa gaagggcggc
     1201 ggcggcggcc cgcagccggc cgagccgcgc tgcgagatca acgaccagaa gtggtacgtc
     1261 atctcgtcgt gcatcggctc cttcttcgct ccctgcctca tcatgatcct ggtctacgtg
     1321 cgcatctacc agatcgccaa gcgtcgcacc cgcgtgccac ccagccgccg gggtccggac
     1381 gccgtcgccg cgccgccggg gggcaccgag cgcaggccca acggtctggg ccccgagcgc
     1441 agcgcgggcc cggggggcgc agaggccgaa ccgctgccca cccagctcaa cggcgcccct
     1501 ggcgagcccg cgccggccgg gccgcgcgac accgacgcgc tggacctgga ggagagctcg
     1561 tcttccgacc acgccgagcg gcctccaggg ccccgcagac ccgagcgcgg tccccggggc
     1621 aaaggcaagg cccgagcgag ccaggtgaag ccgggcgaca gcctgccgcg gcgcgggccg
     1681 ggggcgacgg ggatcgggac gccggctgca gggccggggg aggagcgcgt cggggctgcc
     1741 aaggcgtcgc gctggcgcgg gcggcagaac cgcgagaagc gcttcacgtt cgtgctggcc
     1801 gtggtcatcg gagtgttcgt ggtgtgctgg ttccccttct tcttcaccta cacgctcacg
     1861 gccgtcgggt gctccgtgcc acgcacgctc ttcaaattct tcttctggtt cggctactgc
     1921 aacagctcgt tgaacccggt catctacacc atcttcaacc acgatttccg ccgcgccttc
     1981 aagaagatcc tctgtcgggg ggacaggaag cggatcgtga cggatccacc ggtcgccacc
     2041 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     2101 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     2161 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     2221 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     2281 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     2341 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     2401 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     2461 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     2521 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     2581 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     2641 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     2701 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
     2761 agcggccgcg actctagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
     2821 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
     2881 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     2941 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     3001 cttaaggcgt aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     3061 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
     3121 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     3181 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
     3241 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
     3301 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     3361 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
     3421 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt
     3481 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     3541 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtcct
     3601 gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     3661 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     3721 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     3781 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     3841 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     3901 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaagatc
     3961 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
     4021 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     4081 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     4141 accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg
     4201 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     4261 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     4321 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     4381 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     4441 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     4501 ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     4561 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     4621 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     4681 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     4741 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
     4801 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg
     4861 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     4921 agcgcgggga tctcatgctg gagttcttcg cccaccctag ggggaggcta actgaaacac
     4981 ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa
     5041 cgcacggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg
     5101 tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac
     5161 cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc
     5221 ctgccatagc ctcaggttac tcatatatac tttagattga tttaaaactt catttttaat
     5281 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     5341 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     5401 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     5461 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     5521 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     5581 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     5641 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     5701 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     5761 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     5821 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     5881 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     5941 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     6001 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     6061 ctgattctgt ggataaccgt attaccgcca tgcat
//
