LOCUS 62056_7385 6109 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6109) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..6109 /mol_type="other DNA" /organism="synthetic DNA construct" enhancer 50..429 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 430..633 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" misc_feature 885..1473 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" polyA_signal 1539..1587 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 1789..2217 /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 2231..2560 /label=SV40 promoter /note="SV40 enhancer and early promoter" CDS 2627..3418 /codon_start=1 /label=NeoR/KanR /note="aminoglycoside phosphotransferase" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" polyA_signal 3597..3730 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3767..3783) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(3791..3807) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(3815..3845) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(3860..3881) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(4169..4757) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(4931..5788) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(5789..5893) /label=AmpR promoter ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg 661 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg 721 actctagagc tagcgtttaa acttaagctt ggtaccgagc tcggatccac tagtccagtg 781 tggtggaatt ctgcagatat ccagcacagt ggcggccgct cgagtctaga gggcccctta 841 agggttcgat ccctaccggt tagtaatgag tttgatatct cgacaatcaa cctctggatt 901 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg 961 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct 1021 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc 1081 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca 1141 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac 1201 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt 1261 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct 1321 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc 1381 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga 1441 cgagtcggat ctccctttgg gccgcctccc cgcctggaaa cgggggaggc taactgaaac 1501 acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa 1561 aacgcacggg tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact 1621 ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc 1681 caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag 1741 gccctgccat agcagatctg cgcagctggg gctctagggg gtatccccac gcgccctgta 1801 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca 1861 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct 1921 ttccccgtca agctctaaat cggggcatcc ctttagggtt ccgatttagt gctttacggc 1981 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat 2041 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc 2101 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgg 2161 ggatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat 2221 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag 2281 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc 2341 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct 2401 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg 2461 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa 2521 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat 2581 atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga 2641 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc 2701 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc 2761 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc 2821 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac 2881 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc 2941 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac 3001 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg 3061 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct 3121 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt 3181 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg 3241 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac 3301 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg 3361 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg 3421 agcgggactc tggggttcgc gaaatgaccg accaagcgac gcccaacctg ccatcacgag 3481 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg 3541 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact 3601 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata 3661 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc 3721 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc 3781 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt 3841 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc 3901 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg 3961 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct 4021 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 4081 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 4141 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 4201 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 4261 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 4321 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt 4381 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 4441 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 4501 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 4561 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg 4621 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 4681 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 4741 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 4801 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 4861 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 4921 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 4981 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 5041 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 5101 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 5161 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 5221 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg 5281 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 5341 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 5401 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 5461 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 5521 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa 5581 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 5641 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 5701 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 5761 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 5821 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 5881 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc 5941 tcccgatccc ctatggtcga ctctcagtac aatctgctct gatgccgcat agttaagcca 6001 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc 6061 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttagg //