LOCUS       pCMV-KPNA2(human)-HA    5342 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V035732
VERSION     V035732
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        27..330
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        331..534
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     intron          672..768
                     /note="modified SV40 intron with splice donor and acceptor
                     sites"
                     /label="SV40 intron"
     CDS             844..2430
                     /label="KPNA2(NM_001320611)"
                     /note="KPNA2(NM_001320611)"
                     /gene="KPNA2"
     CDS             2440..2466
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     polyA_signal    2481..2615
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(2726..2742)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    2750..2766
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(2774..2804)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    2819..2840
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(3128..3716)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(3887..4747)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(4748..4852)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     5326..5342
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 gagttcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc
       61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
      541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
      601 cgggaccgat ccagcctccg gactctagag gatccggtac tagaggaact gaaaaaccag
      661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg
      721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa
      781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg ggcccaggcc cgaattcgcc
      841 accatgtcca ccaacgagaa tgctaataca ccagctgccc gtcttcacag attcaagaac
      901 aagggaaaag acagtacaga aatgaggcgt cgcagaatag aggtcaatgt ggagctgagg
      961 aaagctaaga aggatgacca gatgctgaag aggagaaatg taagctcatt tcctgatgat
     1021 gctacttctc cgctgcagga aaaccgcaac aaccagggca ctgtaaattg gtctgttgat
     1081 gacattgtca aaggcataaa tagcagcaat gtggaaaatc agctccaagc tactcaagct
     1141 gccaggaaac tactttccag agaaaaacag ccccccatag acaacataat ccgggctggt
     1201 ttgattccga aatttgtgtc cttcttgggc agaactgatt gtagtcccat tcagtttgaa
     1261 tctgcttggg cactcactaa cattgcttct gggacatcag aacaaaccaa ggctgtggta
     1321 gatggaggtg ccatcccagc attcatttct ctgttggcat ctccccatgc tcacatcagt
     1381 gaacaagctg tctgggctct aggaaacatt gcaggtgatg gctcagtgtt ccgagacttg
     1441 gttattaagt acggtgcagt tgacccactg ttggctctcc ttgcagttcc tgatatgtca
     1501 tctttagcat gtggctactt acgtaatctt acctggacac tttctaatct ttgccgcaac
     1561 aagaatcctg cacccccgat agatgctgtt gagcagattc ttcctacctt agttcggctc
     1621 ctgcatcatg atgatccaga agtattagca gatacctgct gggctatttc ctaccttact
     1681 gatggtccaa atgaacgaat tggcatggtg gtgaaaacag gagttgtgcc ccaacttgtg
     1741 aagcttctag gagcttctga attgccaatt gtgactcctg ccctaagagc catagggaat
     1801 attgtcactg gtacagatga acagactcag gttgtgattg atgcaggagc actcgccgtc
     1861 tttcccagcc tgctcaccaa ccccaaaact aacattcaga aggaagctac gtggacaatg
     1921 tcaaacatca cagccggccg ccaggaccag atacagcaag ttgtgaatca tggattagtc
     1981 ccattccttg tcagtgttct ctctaaggca gattttaaga cacaaaagga agctgtgtgg
     2041 gccgtgacca actataccag tggtggaaca gttgaacaga ttgtgtacct tgttcactgt
     2101 ggcataatag aaccgttgat gaacctctta actgcaaaag ataccaagat tattctggtt
     2161 atcctggatg ccatttcaaa tatctttcag gctgctgaga aactaggtga aactgagaaa
     2221 cttagtataa tgattgaaga atgtggaggc ttagacaaaa ttgaagctct acaaaaccat
     2281 gaaaatgagt ctgtgtataa ggcttcgtta agcttaattg agaagtattt ctctgtagag
     2341 gaagaggaag atcaaaacgt tgtaccagaa actacctctg aaggctacac tttccaagtt
     2401 caggatgggg ctcctgggac ctttaacttt ggtaccggct acccatacga tgttccagat
     2461 tacgcttaag cggccgcggg gatccagaca tgataagata cattgatgag tttggacaaa
     2521 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
     2581 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
     2641 tgtttcaggt tcagggggag gtgtgggagg ttttttcgga tcctctagag tcgatctgca
     2701 ggcatgctag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     2761 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     2821 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     2881 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     2941 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     3001 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     3061 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     3121 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     3181 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     3241 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     3301 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     3361 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     3421 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     3481 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     3541 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     3601 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     3661 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     3721 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     3781 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     3841 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     3901 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     3961 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     4021 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     4081 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     4141 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     4201 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     4261 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     4321 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     4381 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     4441 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     4501 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     4561 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     4621 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     4681 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     4741 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     4801 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     4861 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     4921 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
     4981 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     5041 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
     5101 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     5161 taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag
     5221 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     5281 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
     5341 gt
//
