LOCUS       V022212                 5503 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V022212
VERSION     V022212
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        27..330
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        331..534
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     intron          672..768
                     /note="modified SV40 intron with splice donor and acceptor
                     sites"
                     /label="SV40 intron"
     CDS             832..855
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     CDS             859..2595
                     /label="Igf2bp3(NM_023670)"
                     /note="Igf2bp3(NM_023670)"
                     /gene="Igf2bp3"
     polyA_signal    2642..2776
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(2887..2903)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    2911..2927
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(2935..2965)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    2980..3001
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(3289..3877)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(4048..4908)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(4909..5013)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     5487..5503
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 gagttcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc
       61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
      541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
      601 cgggaccgat ccagcctccg gactctagag gatccggtac tagaggaact gaaaaaccag
      661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg
      721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa
      781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg ggcccaccat ggactacaag
      841 gacgacgatg acaagcttat gaacaaattg tacatcggga acctcagcga ccacgccggc
      901 cccgcggacc tggaaagtgt cttcaaggac gctaagatcc cggtggcggg ccccttcctg
      961 gtgaagacgg gctacgcgtt cgtggactgc ccggacgagg gctgggccct caaggccatc
     1021 gaggcgcttt caggtaaaat ggaactacat gggaaaccga tggaagttga gcactcggtc
     1081 cctaaacggc agaggattcg taaacttcag atacgaaata tcccgcccca cttacaatgg
     1141 gaggtgctgg atagtttact agtccagtat ggagtggtgg agagctgtga gcaagtgaac
     1201 acggattcgg aaacggcagt tgtaaatgta acctattcca gtaaggacca agctagacaa
     1261 gccttagaca aactgaatgg attccagtta gagaacttca ccttgaaggt tgcctacatc
     1321 ccagatgaaa ctgctgccca gcaaaatccc tcgccacagc tccgggggcg ccgggggcca
     1381 gggcagcggg gctcatccag gcaggcgtct ccaggatcgg tgtccaagca gaaaccctgt
     1441 gacctgccac tgcgcctgct ggttcccacc cagtttgttg gagccattat aggaaaagaa
     1501 ggtgctacca ttcgcaacat cacaaagcag acccagtcta aaatcgatgt ccatcgtaag
     1561 gagaatacag gggccgcgga gaagtccatt actatcctct ctacccctga aggcacctct
     1621 gcagcttgta agtctattct ggagattatg cataaggaag ctcaagatat aaaattcaca
     1681 gaggagattc ccttgaagat cttagctcac aataactttg taggccgtct cattggtaaa
     1741 gaaggaagaa accttaaaaa aatcgagcaa gacacggaca ctaaaatcac aatatctcca
     1801 ttgcaggaac tgacgctgta caatccggaa cgcaccatta cagtgaaagg cagtgttgag
     1861 acgtgtgcca aggcggagga ggaaataatg aagaagatca gggagtctta tgaaaatgat
     1921 attgcttcca tgaatcttca agcacattta atccctggat taaatctgaa tgccttgggt
     1981 ctgttcccac ccacgtcagg gatgccacct cccacctcag ggcccccttc aaccctgact
     2041 cctccctacc cacaatttga gcaatcagag acggagactg tgcatctgtt tattcccgcc
     2101 ctgtccgttg gcgccatcat tggcaagcag ggccaacaca tcaaacagct ttctcgcttt
     2161 gcgggagctt cgattaagat cgctccagcc gaagcaccag atgctaaagt gcggatggtg
     2221 attatcactg gaccaccaga ggctcagttc aaggctcagg gaagaattta tggaaaaatt
     2281 aaagaagaaa actttgttag tcctaaagaa gaggtgaaac ttgaagctca catcagagtg
     2341 ccgtcctttg ctgctggcag agttattggg aaaggaggca aaacggtgaa tgagctccag
     2401 agtttatcaa gtgctgaagt tgtcgtcccc cgtgaccaga cacctgatga gaatgatcaa
     2461 gtagttgtca aaataactgg ccacttctat gcttgccagg ttgcccagag gaaaattcag
     2521 gaaattctga ctcaggtaaa gcagcaccag cagcagaaag ctctgcagag tggaccacct
     2581 cagtcaaggc ggaagtaaga attcggtcga ccgagatctc tcgaggtacc gcggccgcgg
     2641 ggatccagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     2701 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     2761 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
     2821 ggtgtgggag gttttttcgg atcctctaga gtcgatctgc aggcatgcta gcttggcgta
     2881 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     2941 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     3001 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     3061 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     3121 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     3181 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     3241 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     3301 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     3361 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     3421 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     3481 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     3541 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     3601 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     3661 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     3721 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     3781 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     3841 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     3901 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     3961 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     4021 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     4081 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     4141 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     4201 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     4261 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     4321 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     4381 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     4441 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     4501 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     4561 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     4621 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     4681 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     4741 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     4801 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     4861 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     4921 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     4981 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     5041 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     5101 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     5161 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     5221 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
     5281 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
     5341 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
     5401 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
     5461 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agt
//
