LOCUS       V033891                 5119 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033891
VERSION     V033891
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        27..330
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        331..534
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     intron          672..768
                     /note="modified SV40 intron with splice donor and acceptor
                     sites"
                     /label="SV40 intron"
     CDS             832..873
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /transl_table=1
                     /translation="GKPIPNPLLGLDST"
                     /label="V5 tag"
     CDS             877..2211
                     /label="HTR7(NM_000872)"
                     /note="HTR7(NM_000872)"
                     /gene="HTR7"
     polyA_signal    2258..2392
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(2503..2519)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    2527..2543
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(2551..2581)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    2596..2617
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(2905..3493)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(3664..4524)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(4525..4629)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     5103..5119
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 gagttcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc
       61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
      541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
      601 cgggaccgat ccagcctccg gactctagag gatccggtac tagaggaact gaaaaaccag
      661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg
      721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa
      781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg ggcccaccat gggtaagcct
      841 atccctaacc ctctcctcgg tctcgattct acgcttatga tggacgttaa cagcagcggc
      901 cgcccggacc tctacgggca cctccgctct ttccttctgc cagaagtggg gcgcgggctg
      961 cccgacttga gccccgacgg tggcgccgac ccggtcgcgg gctcctgggc gccgcacctg
     1021 ctgagcgagg tgacagccag cccggcgccc acctgggacg cgcccccgga caatgcctcc
     1081 ggctgtgggg aacagatcaa ctacggcaga gtcgagaaag ttgtgatcgg ctccatcctg
     1141 acgctcatca cgctgctgac gatcgcgggc aactgcctgg tggtgatctc cgtgtgcttc
     1201 gtcaagaagc tccgccagcc ctccaactac ctgatcgtgt ccctggcgct ggccgacctc
     1261 tcggtggctg tggcggtcat gcccttcgtc agcgtcaccg acctcatcgg gggcaagtgg
     1321 atctttggac actttttctg taatgtcttc atcgccatgg acgtcatgtg ctgcacggcc
     1381 tcgatcatga ccctgtgcgt gatcagcatt gacaggtacc ttgggatcac aaggcccctc
     1441 acataccctg tgaggcagaa tgggaaatgc atggcgaaga tgattctctc cgtctggctt
     1501 ctctccgcct ccatcacctt acctccactc tttggatggg ctcagaatgt aaatgatgat
     1561 aaggtgtgct tgatcagcca ggactttggc tatacgattt actctaccgc agtggcattt
     1621 tatatcccca tgtccgtcat gcttttcatg tactaccaga tttacaaggc tgccaggaag
     1681 agtgctgcca aacacaagtt tcctggcttc cctcgagtgg agccagacag cgtcatcgcc
     1741 ctgaatggca tagtgaagct ccagaaggag gtggaagagt gtgcaaacct ttcgagactc
     1801 ctcaagcatg aaaggaaaaa catctccatc tttaagcgag aacagaaagc agccaccacc
     1861 ctggggatca tcgtcggggc ctttaccgtg tgctggctgc catttttcct cctctcgaca
     1921 gccagaccct tcatctgtgg cacttcctgc agctgcatcc cactgtgggt ggagaggaca
     1981 tttctgtggc taggctatgc aaactctctc attaaccctt ttatatatgc cttcttcaac
     2041 cgggacctga ggaccaccta tcgcagcctg ctccagtgcc agtaccggaa tatcaaccgg
     2101 aagctctcag ctgcaggcat gcatgaagcc ctgaagcttg ctgagaggcc agagagacct
     2161 gagtttgtgc tacaaaatgc tgactactgt agaaaaaaag gtcatgattc atgagaattc
     2221 ggtcgaccga gatctctcga ggtaccgcgg ccgcggggat ccagacatga taagatacat
     2281 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     2341 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     2401 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttcggatcc
     2461 tctagagtcg atctgcaggc atgctagctt ggcgtaatca tggtcatagc tgtttcctgt
     2521 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     2581 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     2641 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     2701 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     2761 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     2821 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     2881 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     2941 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     3001 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     3061 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     3121 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     3181 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     3241 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     3301 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     3361 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     3421 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     3481 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     3541 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     3601 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     3661 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     3721 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     3781 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     3841 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     3901 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     3961 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     4021 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     4081 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     4141 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     4201 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     4261 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     4321 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     4381 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     4441 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     4501 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     4561 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     4621 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
     4681 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga
     4741 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
     4801 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
     4861 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
     4921 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct
     4981 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     5041 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     5101 ttgtaaaacg acggccagt
//
