LOCUS       pCMV-HSF1(human)        5326 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V028938
VERSION     V028938
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        27..330
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        331..534
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     intron          672..768
                     /note="modified SV40 intron with splice donor and acceptor
                     sites"
                     /label="SV40 intron"
     CDS             864..2450
                     /label="HSF1(NM_005526)"
                     /note="HSF1(NM_005526)"
                     /gene="HSF1"
     polyA_signal    2465..2599
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(2710..2726)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    2734..2750
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(2758..2788)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    2803..2824
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(3112..3700)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(3871..4731)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(4732..4836)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     5310..5326
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 gagttcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc
       61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
      541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
      601 cgggaccgat ccagcctccg gactctagag gatccggtac tagaggaact gaaaaaccag
      661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg
      721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa
      781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg ggcccaggcc cgaattcggt
      841 cgaccgagat ctctcgagcc accatggatc tgcccgtggg ccccggcgcg gcggggccca
      901 gcaacgtccc ggccttcctg accaagctgt ggaccctcgt gagcgacccg gacaccgacg
      961 cgctcatctg ctggagcccg agcgggaaca gcttccacgt gttcgaccag ggccagtttg
     1021 ccaaggaggt gctgcccaag tacttcaagc acaacaacat ggccagcttc gtgcggcagc
     1081 tcaacatgta tggcttccgg aaagtggtcc acatcgagca gggcggcctg gtcaagccag
     1141 agagagacga cacggagttc cagcacccat gcttcctgcg tggccaggag cagctccttg
     1201 agaacatcaa gaggaaagtg accagtgtgt ccaccctgaa gagtgaagac ataaagatcc
     1261 gccaggacag cgtcaccaag ctgctgacgg acgtgcagct gatgaagggg aagcaggagt
     1321 gcatggactc caagctcctg gccatgaagc atgagaatga ggctctgtgg cgggaggtgg
     1381 ccagccttcg gcagaagcat gcccagcaac agaaagtcgt caacaagctc attcagttcc
     1441 tgatctcact ggtgcagtca aaccggatcc tgggggtgaa gagaaagatc cccctgatgc
     1501 tgaacgacag tggctcagca cattccatgc ccaagtatag ccggcagttc tccctggagc
     1561 acgtccacgg ctcgggcccc tactcggccc cctccccagc ctacagcagc tccagcctct
     1621 acgcccctga tgctgtggcc agctctggac ccatcatctc cgacatcacc gagctggctc
     1681 ctgccagccc catggcctcc cccggcggga gcatagacga gaggccccta tccagcagcc
     1741 ccctggtgcg tgtcaaggag gagcccccca gcccgcctca gagcccccgg gtagaggagg
     1801 cgagtcccgg gcgcccatct tccgtggaca ccctcttgtc cccgaccgcc ctcattgact
     1861 ccatcctgcg ggagagtgaa cctgcccccg cctccgtcac agccctcacg gacgccaggg
     1921 gccacacgga caccgagggc cggcctccct cccccccgcc cacctccacc cctgaaaagt
     1981 gcctcagcgt agcctgcctg gacaagaatg agctcagtga ccacttggat gctatggact
     2041 ccaacctgga taacctgcag accatgctga gcagccacgg cttcagcgtg gacaccagtg
     2101 ccctgctgga cctgttcagc ccctcggtga ccgtgcccga catgagcctg cctgaccttg
     2161 acagcagcct ggccagtatc caagagctcc tgtctcccca ggagcccccc aggcctcccg
     2221 aggcagagaa cagcagcccg gattcaggga agcagctggt gcactacaca gcgcagccgc
     2281 tgttcctgct ggaccccggc tccgtggaca ccgggagcaa cgacctgccg gtgctgtttg
     2341 agctgggaga gggctcctac ttctccgaag gggacggctt cgccgaggac cccaccatct
     2401 ccctgctgac aggctcggag cctcccaaag ccaaggaccc cactgtctcc taggcggccg
     2461 cggggatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     2521 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     2581 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
     2641 ggaggtgtgg gaggtttttt cggatcctct agagtcgatc tgcaggcatg ctagcttggc
     2701 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     2761 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     2821 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     2881 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     2941 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     3001 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     3061 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     3121 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     3181 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     3241 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     3301 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     3361 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     3421 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     3481 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     3541 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     3601 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     3661 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     3721 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     3781 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     3841 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     3901 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     3961 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     4021 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     4081 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     4141 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     4201 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     4261 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     4321 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     4381 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     4441 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     4501 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     4561 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     4621 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     4681 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     4741 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     4801 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     4861 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     4921 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
     4981 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
     5041 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
     5101 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     5161 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
     5221 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
     5281 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagt
//
