LOCUS       V027580                 9583 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V027580
VERSION     V027580
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        863..881
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             925..5535
                     /label="DOT1L(NM_032482)"
                     /note="DOT1L(NM_032482)"
                     /gene="DOT1L"
     CDS             5548..5613
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     polyA_signal    5648..5872
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      5918..6346
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        6360..6689
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             6701..7099
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /note="confers resistance to blasticidin"
                     /product="blasticidin S deaminase"
                     /transl_table=1
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG*"
                     /label="BSD"
     polyA_signal    7259..7380
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(7429..7445)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7453..7469
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7477..7507)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7522..7543
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7831..8416)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8587..9447)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9448..9552)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 aagctttcta gagaattcgc caccatgggg gagaagctgg agctgagact gaagtcgccc
      961 gtgggggctg agcccgccgt ctacccgtgg ccgctgccgg tctacgataa acatcacgat
     1021 gctgctcatg aaatcatcga gaccatccga tgggtctgtg aagaaatccc ggatctcaag
     1081 ctcgctatgg agaattacgt tttaattgac tatgacacca aaagcttcga gagcatgcag
     1141 aggctctgcg acaagtacaa ccgtgccatc gacagcatcc accagctgtg gaagggcacc
     1201 acgcagccca tgaagctgaa cacgcggccg tccactggac tcctgcgcca tatcctgcag
     1261 caggtctaca accactcggt gaccgacccc gagaagctca acaactacga gcccttctcc
     1321 cccgaggtgt acggggagac ctccttcgac ctggtggccc agatgattga tgagatcaag
     1381 atgaccgacg acgacctgtt tgtggacttg gggagcggtg tgggccaggt cgtgctccag
     1441 gttgctgctg ccaccaactg caaacatcac tatggcgtcg agaaagcaga catcccggcc
     1501 aagtatgcgg agaccatgga ccgcgagttc aggaagtgga tgaaatggta tggaaaaaag
     1561 catgcagaat acacattgga gagaggcgat ttcctctcag aagagtggag ggagcgaatc
     1621 gccaacacga gtgttatatt tgtgaataat tttgcctttg gtcctgaggt ggatcaccag
     1681 ctgaaggagc ggtttgcaaa catgaaggaa ggtggcagaa tcgtgtcctc gaaacccttt
     1741 gcacctctga acttcagaat aaacagtaga aacttgagtg acatcggcac catcatgcgc
     1801 gtggtggagc tctcgcccct gaagggctcg gtgtcgtgga cggggaagcc agtctcctac
     1861 tacctgcaca ctatcgaccg caccatactt gaaaactatt tttctagtct gaaaaaccca
     1921 aaactcaggg aggaacagga ggcagcccgg cgccgccagc agcgcgagag caagagcaac
     1981 gcggccacgc ccactaaggg cccagagggc aaggtggccg gccccgccga cgcccccatg
     2041 gactctggtg ctgaggaaga gaaggcggga gcagccaccg tgaagaagcc gtctccctcc
     2101 aaagcccgca agaagaagct aaacaagaag gggaggaaga tggctggccg caagcgcggg
     2161 cgccccaaga agatgaacac tgcgaacccc gagcggaagc ccaagaagaa ccaaactgca
     2221 ctggatgccc tgcacgctca gaccgtgtct cagacggcgg cctcctcacc ccaggatgcc
     2281 tacagatccc ctcacagccc gttctaccag ctacctccga gcgtgcagcg gcactccccc
     2341 aacccgctgc tggtggcgcc caccccgccc gcgctgcaga agcttctaga gtccttcaag
     2401 atccagtacc tgcagttcct ggcatacaca aagacccccc agtacaaggc cagcctgcag
     2461 gagctgctgg gccaggagaa ggagaagaac gcccagctcc tgggtgcggc tcagcagctc
     2521 ctcagccact gccaggccca gaaggaggag atcaggaggc tgtttcagca aaaattggat
     2581 gagctgggtg tgaaggcgct gacctacaac gacctgattc aagcgcagaa ggagatctcc
     2641 gcccataacc agcagctgcg ggagcagtcg gagcagctgg agcaggacaa ccgcgcgctc
     2701 cgcggccaga gcttgcagct gctcaaggct cgctgcgagg agctgcagct ggactgggcc
     2761 acgctgtcgc tggagaagct gttgaaggag aagcaggccc tgaagagcca gatctcggag
     2821 aagcagaggc actgcctgga gctgcagatc agcattgtgg agctagagaa gagccagcgg
     2881 cagcaggagc tcctgcagct caagtcctgt gtgccgcctg acgacgccct gtccctgcac
     2941 ctgcgtggga agggcgccct gggccgcgag ctggagcctg acgccagccg gctgcacctg
     3001 gagctggact gcaccaagtt ctcgctgcct cacttgagca gcatgagccc ggagctctcc
     3061 atgaacggcc aggctgctgg ctatgagctc tgcggtgtgc tgagccggcc ttcgtcgaag
     3121 cagaacacgc cccagtacct ggcctcaccc ctggaccagg aggtggtgcc ctgtacccct
     3181 agccacgtcg gccggccgcg cctggagaag ctgtctggcc tagccgcacc cgactacact
     3241 aggctgtccc cggccaagat tgtgctgagg cggcacctga gccaggacca cacggtgccc
     3301 ggcaggccgg ctgccagtga gctgcattcg agagctgagc acaccaagga gaacggcctt
     3361 ccctaccaga gccccagcgt gcctggcagc atgaagctga gccctcagga cccgcggccc
     3421 ctgtcccctg gggccttgca gcttgctgga gagaagagca gtgagaaggg cctgagagag
     3481 cgcgcctacg gcagcagcgg ggagctcatc accagcctgc ccatcagcat cccgctcagc
     3541 accgtgcagc ccaacaagct cccggtcagc attcccctgg ccagcgtggt gctgcccagc
     3601 cgcgccgaga gggcgaggag cacccccagt cccgtgctgc agccccgtga cccctcgtcc
     3661 acacttgaaa agcagattgg tgctaatgcc cacggtgctg ggagcagaag ccttgccctg
     3721 gcccccgcag gcttctccta cgctggctcg gtggccatca gcggggcctt ggcgggcagc
     3781 ccggcctctc tcacacctgg agccgagccg gccaccttgg atgagtcctc cagctctggg
     3841 agcctttttg ccaccgtggg gtcccgcagc tccacgccac agcaccccct gctgctggca
     3901 cagccccgga actcgcttcc tgcctctccc gcccaccagc tctcctccag tccccggctt
     3961 ggtggggccg cccagggccc gttgcccgag gccagcaagg gagacctgcc ctccgattcc
     4021 ggcttctcag atcctgagag tgaagccaag aggaggattg tgttcaccat caccactggt
     4081 gcgggcagtg ccaagcagtc gccctccagc aagcacagcc ccctgaccgc cagcgcccgt
     4141 ggggactgtg tgccgagcca cgggcaggac agtcgcaggc gcggccggcg gaagcgagca
     4201 tctgcgggga cgcccagctt gagcgcaggc gtgtccccca agcgccgagc cctgccgtcc
     4261 gtcgctggcc ttttcacaca gccttcgggg tctcccctca acctcaactc catggtcagt
     4321 aacatcaacc agcccctgga gattacagcc atctcgtccc cggagacctc cctgaagagc
     4381 tcccctgtgc cctaccagga ccacgaccag ccccccgtgc tcaagaagga gcggcctctg
     4441 agccagacca atggggcaca ctactcccca ctcacctcag acgaggagcc aggctctgag
     4501 gacgagccca gcagtgctcg aattgagaga aaaattgcaa caatctcctt agaaagcaaa
     4561 tctcccccga aaaccttgga aaatggtggt ggcttggcgg gaaggaagcc cgcgcccgcc
     4621 ggcgagccag tcaatagcag caagtggaag tccaccttct cgcccatctc cgacatcggc
     4681 ctggccaagt cggcggacag cccgctgcag gccagctccg ccctcagcca gaactccctg
     4741 ttcacgttcc ggcccgccct ggaggagccc tctgccgatg ccaagctggc cgctcacccc
     4801 aggaaaggct ttcccggctc cctgtcgggg gctgacggac tcagcccggg caccaaccct
     4861 gccaacggct gcaccttcgg cgggggcctg gccgcggacc tgagtttaca cagcttcagt
     4921 gatggtgctt ctcttcccca caagggcccc gaggcggccg gcctgagctc cccgctgagc
     4981 ttcccctcgc agcgcggcaa ggagggctcg gacgccaacc ctttcctgag caagaggcag
     5041 ctggacggcc tggctgggct gaagggcgag ggcagccgcg gcaaggaggc aggggagggc
     5101 ggcctaccgc tgtgcgggcc cacggacaag accccactgc tgagcggcaa ggccgccaag
     5161 gcccgggacc gcgaggtcga cctcaagaat ggccacaacc tcttcatctc tgcggcggcc
     5221 gtgcctcccg gaagcctcct cagcggcccc ggcctggccc cggcggcgtc ctccgcaggc
     5281 ggcgcggcgt cctccgccca gacgcaccgg tccttcctgg gccccttccc gccgggaccg
     5341 cagttcgcgc tcggccccat gtccctgcag gccaacctcg gctccgtggc cggctcctcc
     5401 gtgctgcagt cgctgttcag ctctgtgccg gccgccgcag gcctggtgca cgtgtcgtcc
     5461 gctgccacca gactgaccaa ctcgcacgcc atgggcagct tttccggggt ggcaggcggc
     5521 acagttggag gtaacctcga gaccggtgac tacaaagacc atgacggtga ttataaagat
     5581 catgacatcg attacaagga tgacgatgac aagtgaccgg taagtttaaa ccgctgatca
     5641 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     5701 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     5761 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     5821 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     5881 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     5941 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     6001 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     6061 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     6121 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     6181 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     6241 acactcaacc ctatctcggt ctattctttt gatttataag ggattttggc catttcggcc
     6301 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     6361 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     6421 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     6481 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     6541 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     6601 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     6661 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg atggccaagc ctttgtctca
     6721 agaagaatcc accctcattg aaagagcaac ggctacaatc aacagcatcc ccatctctga
     6781 agactacagc gtcgccagcg cagctctctc tagcgacggc cgcatcttca ctggtgtcaa
     6841 tgtatatcat tttactgggg gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc
     6901 tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt
     6961 gagcccctgc ggacggtgcc gacaggtgct tctcgatctg catcctggga tcaaagccat
     7021 agtgaaggac agtgatggac agccgacggc agttgggatt cgtgaattgc tgccctctgg
     7081 ttatgtgtgg gagggctaat tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     7141 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     7201 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
     7261 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     7321 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     7381 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     7441 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     7501 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     7561 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     7621 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     7681 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     7741 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     7801 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     7861 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     7921 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     7981 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     8041 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     8101 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     8161 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     8221 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     8281 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     8341 cggcaaacaa accaccgctg gtagcggttt ttttgtttgc aagcagcaga ttacgcgcag
     8401 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     8461 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     8521 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     8581 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     8641 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     8701 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     8761 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     8821 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     8881 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     8941 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     9001 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     9061 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     9121 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     9181 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     9241 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     9301 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     9361 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     9421 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     9481 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     9541 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtc
//
