LOCUS       pCMV-Bbs2(mouse)-HA     5930 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033668
VERSION     V033668
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        27..330
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        331..534
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     intron          672..768
                     /note="modified SV40 intron with splice donor and acceptor
                     sites"
                     /label="SV40 intron"
     CDS             832..858
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             892..3054
                     /label="Bbs2(NM_026116)"
                     /note="Bbs2(NM_026116)"
                     /gene="Bbs2"
     polyA_signal    3069..3203
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(3314..3330)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    3338..3354
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(3362..3392)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    3407..3428
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(3716..4304)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(4475..5335)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(5336..5440)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     5914..5930
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 gagttcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc
       61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
      541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
      601 cgggaccgat ccagcctccg gactctagag gatccggtac tagaggaact gaaaaaccag
      661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg
      721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa
      781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg ggcccaccat gtacccatac
      841 gatgttccag attacgctct tatggccatg gaggcccgaa ttcggtcgac aatgctgctg
      901 cctgtgttca ctctgaaact gcgccacaaa atcagccccc gcatggtggc catagggcgc
      961 tacgacggga ctcacccgtg cttggctgcc gccacccaag cgggcaaggt tttcattcat
     1021 aaccctcaca cgcggagcca gcatttcagt gcttccagag tgttccagag ccctctggag
     1081 tctgatgtct ctctgctcaa cattaaccag acggtcagct gcctgggttc gggagtcttg
     1141 aaccctgagc ttggctatga tactctttta gtggggacac agaccagtct tttggcttac
     1201 gacatctaca ataattcaga tttgttctac agagaggtct cagatggggc aaatgccatt
     1261 gtgctgggga ccctgggaga cattgcccca cctcttgcaa tcatcggtgg aaactgtgct
     1321 ctgcagggtt tcgatcacga aggaaatgat ctcttttgga cggttactgg agacaatgtg
     1381 cattccttgg ctctgtgtga ctttgacggt gatgggaaga cagagcttct tgttggatct
     1441 gaagattttg acattcgagt ttttaaagaa gatgagattg tggcagaaat gacagagaca
     1501 gagatagtca cctctctgtg tcctatgtat ggcagtcggt ttggttacgc cctttctaat
     1561 ggcacagttg gagtttatga caaaacagcc cggtactgga gaattaaatc caaaaaccat
     1621 gccatgagca tccatgcttt tgacattaat tctgatggag tgtgtgaact gataaccggg
     1681 tggtctaatg ggaaggttga tgctcgcagt gaccggactg gagaggtcat atttaaagac
     1741 aacttctctt ctgctgttgc tggtgtggta gagggagatt accggatgga tggccacgta
     1801 cagttaatct gctgctcagt ggatggggaa atccgaggct acctgccagg cacagctgag
     1861 atgaagggga acctcctgga cacaagtgtg gagcaggacc tgatccgaga gctgagtcag
     1921 aaaaagcaga acctgttgct agaacttcgc aactatgagg agagcaccaa ggcagaactg
     1981 agcagtcccc tgaacgaggc cgatgggcag aaaggcataa tcccagccaa taccaggctc
     2041 cacacagcgc tctcagtcaa tatggggaat gacttacaag atgcgcatgc ggaattaggc
     2101 atttccactt ccaatgatac tatcatccga gcagtactaa tttttgcaga gggaattttt
     2161 gtgggtgaaa gccatgtggt ccaccccagc attcacaacc tttccagctc cctccgggta
     2221 ccaattacac ctcccaaaga tgtccctgtg gatctgcacc tgaaaacctt cgtgggttac
     2281 agaagcagca cccagttcca tgtgtttgaa ctgacaagac agctgccccg attcaccatg
     2341 tatgcattga caagcccaga tgcagctagc gagcctgtca gctacgtgaa cttcagtgtt
     2401 gccgagcgca cacagaggat ggtcacgtgg ctcaaccaga actttctgtt gccagaagac
     2461 agtaacgttc agaattcccc gtttcacgtc tgtttcacat cgttacgcaa tggtggccag
     2521 ctctacataa aaatgaaaca aagtggtgag atcactgtga atactgacga tattgatcta
     2581 gctggtgaca tcatccagtc gatcgcgtcc ttttttgcta ttgaagacct tcaggtagaa
     2641 gcagatttcc ctgtgtactt cgaggaacta cggaaagtgc tggtgaaggt ggatgagtat
     2701 cattcagtgc atcaaaagct cagcgccgac atggctgata attctaacct catccgaagt
     2761 ttgctggtca gagcggagga tgctcggctg atgagggaca tgaaaacaat gaagagtcgt
     2821 tacatggagc tctatgacct taataaagat ttgctgaatg gatacaagat tcgctgtaac
     2881 aaccacacag aactgctagg aaacctgaag gcggtgaacc aagcaatcca aagagcaggc
     2941 cgtctgcgcg ttggaaagcc aaagaaccag gtgatcagtg cttgtcggga tgcgattcga
     3001 agcaacaaca tcaacaccct cttcagaatc atgcgagtgg gcactgctcc ttcataggcg
     3061 gccgcgggga tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     3121 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     3181 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     3241 agggggaggt gtgggaggtt ttttcggatc ctctagagtc gatctgcagg catgctagct
     3301 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     3361 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     3421 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     3481 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     3541 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     3601 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     3661 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     3721 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     3781 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     3841 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     3901 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     3961 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     4021 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     4081 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     4141 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     4201 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     4261 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     4321 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     4381 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     4441 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     4501 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     4561 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     4621 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     4681 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     4741 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     4801 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     4861 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     4921 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     4981 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     5041 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     5101 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     5161 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     5221 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     5281 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     5341 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     5401 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     5461 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     5521 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     5581 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     5641 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
     5701 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     5761 accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     5821 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
     5881 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
//
