LOCUS V040462 6166 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V040462 VERSION V040462 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 61..364 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 365..568 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 635..1216 /label="BNIP3(NM_004052)" /note="BNIP3(NM_004052)" /gene="BNIP3" CDS 1229..1294 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" polyA_signal 1335..1559 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" promoter 1575..2098 /label="mCMV promoter" CDS 2112..2831 /codon_start=1 /note="mammalian codon-optimized" /product="the original enhanced GFP (Yang et al., 1996)" /transl_table=1 /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV LLEFVTAAGITLGMDELYK*" /label="EGFP" polyA_signal 2954..3075 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(3082..3537) /direction=LEFT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3564..3668 /gene="bla" /label="AmpR promoter" promoter 3670..4027 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4062..4856 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5088..5135 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 5464..6052 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgagct ccgaattcgc caccatgtcg cagaacggag cgcccgggat 661 gcaggaggag agcctgcagg gctcctgggt agaactgcac ttcagcaata atgggaacgg 721 gggcagcgtt ccagcctcgg tttctattta taatggagac atggaaaaaa tactgctgga 781 cgcacagcat gagtctggac ggagtagctc caagagctct cactgtgaca gcccacctcg 841 ctcgcagaca ccacaagata ccaacagagc ttctgaaaca gatacccata gcattggaga 901 gaaaaacagc tcacagtctg aggaagatga tattgaaaga aggaaagaag ttgaaagcat 961 cttgaagaaa aactcagatt ggatatggga ttggtcaagt cggccggaaa atattccccc 1021 caaggagttc ctctttaaac acccgaagcg cacggccacc ctcagcatga ggaacacgag 1081 cgtcatgaag aaagggggca tattctctgc agaatttctg aaagttttcc ttccatctct 1141 gctgctctct catttgctgg ccatcggatt ggggatctat attggaaggc gtctgacaac 1201 ctccaccagc acctttaagc ttacgcgtga ctacaaagac catgacggtg attataaaga 1261 tcatgacatc gactacaagg atgacgatga caagtgagga tccgggcccg tttaaacccg 1321 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt 1381 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat 1441 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag 1501 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggt 1561 ctagagatat actgagtcat tagggacttt ccaatgggtt ttgcccagta cataaggtca 1621 ataggggtga atcaacagga aagtcccatt ggagccaagt acactgagtc aatagggact 1681 ttccattggg ttttgcccag tacaaaaggt caataggggg tgagtcaatg ggtttttccc 1741 attattggca cgtacataag gtcaataggg gtgagtcatt gggtttttcc agccaattta 1801 attaaaacgc catgtacttt cccaccattg acgtcaatgg gctattgaaa ctaatgcaac 1861 gtgaccttta aacggtactt tcccatagct gattaatggg aaagtaccgt tctcgagcca 1921 atacacgtca atgggaagtg aaagggcagc caaaacgtaa caccgccccg gttttcccct 1981 ggaaattcca tattggcacg cattctattg gctgagctgc gttctacgtg ggtataagag 2041 gcgcgaccag cgtcggtacc gtcgcagtct tcggtctgac caccgtagaa cgcagatcac 2101 cggtcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg 2161 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg 2221 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc 2281 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg 2341 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc 2401 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg 2461 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca 2521 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca 2581 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg 2641 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc 2701 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg 2761 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc 2821 tgtacaagta aagcggccgc gactctagat cataatcagc cataccacat ttgtagaggt 2881 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc 2941 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat 3001 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact 3061 catcaatgta tcttaaggcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa 3121 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata 3181 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac 3241 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc 3301 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa 3361 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg 3421 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg 3481 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag 3541 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 3601 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 3661 ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa 3721 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac 3781 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa 3841 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag 3901 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc 3961 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt 4021 ttgcaaagat cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat 4081 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac 4141 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc 4201 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc 4261 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag 4321 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc 4381 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg 4441 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc 4501 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc 4561 cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga 4621 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca 4681 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg 4741 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg 4801 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg 4861 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga 4921 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg 4981 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct 5041 aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag 5101 acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg 5161 ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc 5221 cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg 5281 ggcggcaggc cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact 5341 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat 5401 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc 5461 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct 5521 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg 5581 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca 5641 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc 5701 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga 5761 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac 5821 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga 5881 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag 5941 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg 6001 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag 6061 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc 6121 tgcgttatcc cctgattctg tggataaccg tattaccgcc atgcat //