LOCUS       pCMV-Armc3(mouse)-HA    6416 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V034499
VERSION     V034499
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        27..330
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        331..534
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     intron          672..768
                     /note="modified SV40 intron with splice donor and acceptor
                     sites"
                     /label="SV40 intron"
     CDS             832..858
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             892..3534
                     /label="Armc3(NM_001081083)"
                     /note="Armc3(NM_001081083)"
                     /gene="Armc3"
     polyA_signal    3555..3689
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(3800..3816)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    3824..3840
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(3848..3878)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    3893..3914
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(4202..4790)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(4961..5821)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(5822..5926)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     6400..6416
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 gagttcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc
       61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
      541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
      601 cgggaccgat ccagcctccg gactctagag gatccggtac tagaggaact gaaaaaccag
      661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg
      721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa
      781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg ggcccaccat gtacccatac
      841 gatgttccag attacgctct tatggccatg gaggcccgaa ttcgggtcga catgggtaaa
      901 aagataaaga aggaagttga acctccccct aaggatgtgt ttgacccaat cacaattgaa
      961 agcaaaaagg cagccactgt ggtgctgatg ctcaagtctc cagaggaaga catcttagcc
     1021 aaagcctgcg aagccatcta taagtttgct ctgaaaggtg aggagaataa agcaactctc
     1081 cttgaacttg gagctgtgga gcctttgacc aaactgctca cccacgaaga caaaactgta
     1141 agaagaaatg ctatgatgat ctttggtatt ctggcttcta acagtgatgt taagaagctc
     1201 ttgagggagt tagaagttat gaattcagtg attgctcagc tctccccaga agaagaagtt
     1261 gtcatccatg agtttgctag tctttgtctt gcgaacatgt ccgtagagta cacagggaaa
     1321 gtccagatat ttgagcatgg tggactggag ccgctcatca gactgctgag cagctcggac
     1381 ccggacgtga agaagaattc tattgagtgc atctacaact tggtacagga ttttcagtgt
     1441 cggactacac ttcaagagct aaatgcaatc cctccgatat tagaactttt gaggtcagag
     1501 taccccatta tccagctgct ggctctcaaa accttgggtg tcatcacgtg cgataaggag
     1561 gctcggacga tgctgaagga aaatcaaggg ctggaccacc tcaccaagat cctggagacc
     1621 aaggaactga atgaccttca tgtagaagca ctctcagtga tagccaactg cctggaggat
     1681 atggacacca tggtgctgat gcagcagaca gggagcctca aaaaagtcct gtcattcgca
     1741 gagagctcca caattcctga cattcagaag aacgcagcca aagctattgc caaagcagct
     1801 tatgaccctg aaaatagaaa agtttttcac gaacaagagg ttgaaaaatg cctggtgact
     1861 ctcctgggat ctgacagcga tggaaccaag attgctgctt cccaggctat ttcagccctg
     1921 tgtgagaact tgagctgcaa agaatttttc aacacccagg ggattccaca gatagttcag
     1981 ttgctcagaa gtgacaatga ggaggtgagg gaagctgccg cactggccct ggcaaacctg
     2041 acgaccagca gccctgcaaa tgccaatgct gctgcagaag cagatgccat tgacccgctg
     2101 attaacatcc tgtcgagcaa gcgggatgga gccattgcca atgctgccac agtgctgacc
     2161 aacatggcca cgcaagagcc tctgcgagct atcatccaga accatgaaat aatgcacgca
     2221 ctgttgggcc ccctgcactc caccaacacc ttggtgcaga gcactgcagc gctcaccgtg
     2281 gctgccactg cctgcgacgt ggaagcccgc acccagttga ggaactgcgg tggtctggtg
     2341 cccctggttg ggctcctgca ctccaagaat gacgaggtac gaaggcatgc cagctgggct
     2401 gtgatggtct gtgccggcga tgagccgatg gcagttgagt tatgcaggct cggggcccta
     2461 aatatccttg aagaaattaa tcgatcatta agtcggaaaa ataaattcag tgaggcagcc
     2521 tataacaaat tgctaaacaa caatctttcc ctgaaataca gccagactgg ctatttgtca
     2581 tcaagcaata taattagcga tggattctat gattacggtc ggataaatcc tggcaccaaa
     2641 cttttgtcac tgaaggaact gtgcttacag gagctgaatg atcaacgtgc tattctatta
     2701 gtcaacaata aatctgacac ctctccacct ccatctatgg aggataaatc atcggacgtt
     2761 ggctatggac gaagcatttc ttcttcatct tccttaagaa ggggaagcaa ggagaaggcc
     2821 aatgctatat ttggatctcc tacagaagaa aaatccgagc cagcatcagt acggaacact
     2881 atccttagca gagctttcac caaagagaag ggagtaagta aaaagaaaag tagacttcag
     2941 ttgatttgtt cctcttacct tctgtggaaa gggaaaggga aaaaagaaga agagaaagtg
     3001 aaagaggaag aagagatcct ggcactgccc aagttcacgg aaggcagccc tgagaaagag
     3061 tggaaccctc ctcctgaccc tgagttctgt gtgtatgtgc tggaggtgac caagtcgata
     3121 ctgcccattg tcaaccttaa ggagcagatt gaggtccttg ccaagtatgt ggcagataaa
     3181 atgggcggta agattccaaa agagaagcta gctgatttca gctgggaact tcatataagt
     3241 gagttgaaat ttcaactgaa atccaatgtc gtaccaattg gatacatcaa gaagggaatc
     3301 ttttatcatc gagcgttgct tttcaaggca ctggctgata aaatcggggt aggctgcagc
     3361 ctggtgcgtg gtgagtacag ccgagggtgg aacgaagtga agctggtgaa tgaggctcgg
     3421 aaaggcatga tcgggaacct gcccccaccc gaagagtaca tcgtggatct catgttccac
     3481 ccggggaacc tgctgaagct gcgcagcaaa gaggctgacc tctaccgctt tctgtagggt
     3541 accgcggccg cggggatcca gacatgataa gatacattga tgagtttgga caaaccacaa
     3601 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     3661 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
     3721 aggttcaggg ggaggtgtgg gaggtttttt cggatcctct agagtcgatc tgcaggcatg
     3781 ctagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     3841 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     3901 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     3961 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     4021 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     4081 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     4141 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     4201 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     4261 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     4321 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     4381 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     4441 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     4501 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     4561 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     4621 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     4681 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     4741 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     4801 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     4861 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     4921 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     4981 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     5041 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     5101 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     5161 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     5221 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     5281 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     5341 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     5401 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     5461 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     5521 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     5581 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     5641 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     5701 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     5761 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     5821 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     5881 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     5941 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     6001 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     6061 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     6121 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
     6181 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
     6241 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat
     6301 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
     6361 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagt
//
