LOCUS       pCMV-Adam3(mouse)-HA    6239 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V036131
VERSION     V036131
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        27..330
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        331..534
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     intron          672..768
                     /note="modified SV40 intron with splice donor and acceptor
                     sites"
                     /label="SV40 intron"
     CDS             832..858
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             892..3357
                     /label="Adam3(NM_009619)"
                     /note="Adam3(NM_009619)"
                     /gene="Adam3"
     polyA_signal    3378..3512
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(3623..3639)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    3647..3663
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(3671..3701)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    3716..3737
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(4025..4613)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(4784..5644)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(5645..5749)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     6223..6239
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 gagttcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc
       61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
      541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
      601 cgggaccgat ccagcctccg gactctagag gatccggtac tagaggaact gaaaaaccag
      661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg
      721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa
      781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg ggcccaccat gtacccatac
      841 gatgttccag attacgctct tatggccatg gaggcccgaa ttcggtcgac catgctgccc
      901 ttattcctag tcctctcata cctaggccaa gtgatcgctg caggcaaaga cgtagaaaca
      961 ccacttctgc agatcacagt accagagaaa atcgacacca acatccagga tgccaaggag
     1021 gcagagacac aagtcactta cgtggtcaga attgagggca aagcatacac actccaactt
     1081 gaaaaacagt catttttaca cccacttttt ggaacatatt tgcgtgacaa gttgggaaca
     1141 ttgcaaccat acttttcttt agtaaagact cactgctttt atcaaggaca tgctgccgag
     1201 atcccagtat caacagtgac actcagcact tgctcaggtc tcaggggttt gttgcagtta
     1261 gagaacatca cttatggaat tgagcccttg gagtcttcag ctacatttga acacatactt
     1321 tatgaaatta agaataacaa aattgactat tctcctttaa aagaaaactt cgcaaattct
     1381 gagcaagaaa gtcagtccta ccgaatcctt gtcaaaccag agaaaggttc gaactcgaca
     1441 ctaacaaaga gaattctgag gataaagatc atcatggata aagccatgtt tgaccatatg
     1501 ggctctgagg tgggagttgc aactcagaaa gttgttcaca tctttggtct cattaatact
     1561 atgttttccc agctgaagat gacagtgatg ttaaactctc tggagatctg gtcagaacaa
     1621 gacaagatag aaaccaatgg ggatgctgat gaggtcctgc aaaggttcct gctgtggaaa
     1681 agcaaggaaa tatctcaaaa ggcccaggat ataacctact tactcctgta taaggatcat
     1741 cctgattatg tgggagcgac atatcatggg atggcctgca acccaaattt taccgcagga
     1801 attgctctgc atccaaagac gttagctgtg gagggctttg ccattgttct ctcacagttg
     1861 ctgggaatta acctggggtt ggcatacgat gacgtctaca attgtttctg tccaggaagc
     1921 acatgcataa tgaatccttc ggcaatacgt tcccaaggca taaaggtttt tagcagttgt
     1981 agtgtggatg aattcaaaca gctggcttca caacctgaac tggactgcct tcggaataca
     2041 tcggagacag aatttgttgt ccagccccaa ggtggatcgt attgtggtaa ccatctcctg
     2101 gaggtcccag agcaatgtga ctgtggccca ccagagacct gtactcataa aaaatgctgt
     2161 aaccctaaag actgtactct gattgatgct gcgcagtgtg gtacagggcc atgctgtgat
     2221 aaaagaacat gtacgatagc cgaaagagga cgcctatgca gaaaaagtaa ggaccagtgt
     2281 gatttcccag agttctgcaa tggagaaact gaaggctgtg cccctgatac aaaagctgca
     2341 gacctggaac cttgcaacaa cgagactgcc tattgctttg gtggggtttg ccgagaccca
     2401 gataggcagt gtacagattt atttgggaaa tatgctaagg gtcctaatta tgtgtgtgca
     2461 caagaagtga atctgcaaaa tgacaaattt ggaaactgtc atgggcgatg taattatagt
     2521 gctatatttt gtggaaaagc agtttgctac tggaactttg cagaagtcat acaaacagag
     2581 aaatatgatg ttcagtatac ttacttggga ggccaggtgt gtgtgtccgc tcatttaaga
     2641 tcacagactg ggactagaga tgacacatat gtccatgatg gcactgtttg tggtagtggc
     2701 caggtttgct tccgaggaga ttgcttacgt gtgcatgtcc taaggggaac cagggagtgt
     2761 gaggccgatg acaaatgcca aggacacggg atttgcaaca atctaaacaa ctgtcagtgt
     2821 gaatctggtt ttgctcctcc agagtgtgac atgacacctt catctccagg aggaagcatg
     2881 gatgacggat tttggctgcc cttcgataaa agcacacctt tgattttcaa gcggcatggc
     2941 cttaagtaca aaaaagtgct tttgatcagt ttctatatcc tcctgccttt ccttgttgtg
     3001 ctagccttca tggctgttaa gcggatgatt ggtaagcggc ttgctaagca gaatatctca
     3061 aaggcattgg agcacaaaga ggaagcattc aacagagggt ccatgaaccc aggcgtggtc
     3121 tccggtggca acacagacca gaacctcatg acagtcccag gtagcttcaa cagctacgca
     3181 tatcatggca acacagacca gaacttcatg acggtcccag gtagcttcaa cagctactca
     3241 tatcatggca acacagacca gaacttcatg acggtcccag gtagcttcaa cagctactca
     3301 tatcaggatg ttccttacta ccgatcaata ccagaagacg gtaacgacag ccagcagtaa
     3361 ggtaccgcgg ccgcggggat ccagacatga taagatacat tgatgagttt ggacaaacca
     3421 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     3481 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
     3541 ttcaggttca gggggaggtg tgggaggttt tttcggatcc tctagagtcg atctgcaggc
     3601 atgctagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     3661 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     3721 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     3781 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     3841 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     3901 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     3961 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     4021 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     4081 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     4141 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     4201 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     4261 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     4321 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     4381 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     4441 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     4501 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     4561 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     4621 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     4681 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     4741 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     4801 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     4861 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     4921 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     4981 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     5041 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     5101 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     5161 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     5221 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     5281 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     5341 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     5401 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     5461 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     5521 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     5581 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     5641 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     5701 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     5761 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     5821 ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac
     5881 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     5941 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat
     6001 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
     6061 ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc
     6121 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     6181 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagt
//
