LOCUS       V041801                 6532 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V041801
VERSION     V041801
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             635..1582
                     /label="AKR1B1(NM_001628)"
                     /note="AKR1B1(NM_001628)"
                     /gene="AKR1B1"
     CDS             1595..1660
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     polyA_signal    1701..1925
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     promoter        1941..2464
                     /label="mCMV promoter"
     CDS             2478..3197
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     polyA_signal    3320..3441
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3448..3903)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3930..4034
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        4036..4393
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4428..5222
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5454..5501
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5830..6418
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct ccgaattcgc caccatggca agccgtctcc tgctcaacaa
      661 cggcgccaag atgcccatcc tggggttggg tacctggaag tcccctccag ggcaggtgac
      721 tgaggccgtg aaggtggcca ttgacgtcgg gtaccgccac atcgactgtg cccatgtgta
      781 ccagaatgag aatgaggtgg gggtggccat tcaggagaag ctcagggagc aggtggtgaa
      841 gcgtgaggag ctcttcatcg tcagcaagct gtggtgcacg taccatgaga agggcctggt
      901 gaaaggagcc tgccagaaga cactcagcga cctgaagctg gactacctgg acctctacct
      961 tattcactgg ccgactggct ttaagcctgg gaaggaattt ttcccattgg atgagtcggg
     1021 caatgtggtt cccagtgaca ccaacattct ggacacgtgg gcggccatgg aagagctggt
     1081 ggatgaaggg ctggtgaaag ctattggcat ctccaacttc aaccatctcc aggtggagat
     1141 gatcttaaac aaacctggct tgaagtataa gcctgcagtt aaccagattg agtgccaccc
     1201 atatctcact caggagaagt taatccagta ctgccagtcc aaaggcatcg tggtgaccgc
     1261 ctacagcccc ctcggctctc ctgacaggcc ctgggccaag cccgaggacc cttctctcct
     1321 ggaggatccc aggatcaagg cgatcgcagc caagcacaat aaaactacag cccaggtcct
     1381 gatccggttc cccatgcaga ggaacttggt ggtgatcccc aagtctgtga caccagaacg
     1441 cattgctgag aactttaagg tctttgactt tgaactgagc agccaggata tgaccacctt
     1501 actcagctac aacaggaact ggagggtctg tgccttgttg agctgtacct cccacaagga
     1561 ttaccccttc catgaagagt ttaagcttac gcgtgactac aaagaccatg acggtgatta
     1621 taaagatcat gacatcgact acaaggatga cgatgacaag tgaggatccg ggcccgttta
     1681 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     1741 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     1801 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     1861 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     1921 tatggtctag agatatactg agtcattagg gactttccaa tgggttttgc ccagtacata
     1981 aggtcaatag gggtgaatca acaggaaagt cccattggag ccaagtacac tgagtcaata
     2041 gggactttcc attgggtttt gcccagtaca aaaggtcaat agggggtgag tcaatgggtt
     2101 tttcccatta ttggcacgta cataaggtca ataggggtga gtcattgggt ttttccagcc
     2161 aatttaatta aaacgccatg tactttccca ccattgacgt caatgggcta ttgaaactaa
     2221 tgcaacgtga cctttaaacg gtactttccc atagctgatt aatgggaaag taccgttctc
     2281 gagccaatac acgtcaatgg gaagtgaaag ggcagccaaa acgtaacacc gccccggttt
     2341 tcccctggaa attccatatt ggcacgcatt ctattggctg agctgcgttc tacgtgggta
     2401 taagaggcgc gaccagcgtc ggtaccgtcg cagtcttcgg tctgaccacc gtagaacgca
     2461 gatcaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     2521 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     2581 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     2641 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
     2701 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     2761 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     2821 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     2881 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
     2941 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
     3001 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     3061 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
     3121 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     3181 acgagctgta caagtaaagc ggccgcgact ctagatcata atcagccata ccacatttgt
     3241 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     3301 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     3361 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     3421 caaactcatc aatgtatctt aaggcgtaaa ttgtaagcgt taatattttg ttaaaattcg
     3481 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     3541 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     3601 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     3661 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
     3721 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     3781 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     3841 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     3901 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     3961 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     4021 gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg
     4081 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     4141 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     4201 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     4261 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     4321 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     4381 aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag
     4441 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     4501 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     4561 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag
     4621 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     4681 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     4741 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     4801 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     4861 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     4921 tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg
     4981 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     5041 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     5101 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     5161 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     5221 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
     5281 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     5341 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg
     5401 gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat
     5461 aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc
     5521 ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt
     5581 ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa
     5641 cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt
     5701 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     5761 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     5821 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     5881 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     5941 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     6001 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     6061 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     6121 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     6181 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     6241 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     6301 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     6361 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     6421 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     6481 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgccatgc at
//
