LOCUS       40924_10316       11877 bp DNA     circular SYN 13-MAY-2021
DEFINITION  Non-integrating (episomal) expression of human SOX2 and KLF4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11877)
  AUTHORS   Okita K, Yamakawa T, Matsumura Y, Sato Y, Amano N, Watanabe A, 
            Goshima N, Yamanaka S
  TITLE     An Efficient Non-viral Method to Generate Integration-Free Human iPS
            Cells from Cord Blood and Peripheral Blood Cells.
  JOURNAL   Stem Cells. 2012 Nov 29. doi: 10.1002/stem.1293.
  PUBMED    23193063
REFERENCE   2  (bases 1 to 11877)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 11877)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cells.
            2012 Nov 29. doi: 10.1002/stem.1293."
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11877
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..659
                     /label=chicken beta-actin promoter
     intron          660..1676
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
     primer_bind     1684..1703
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids,
                     forward primer"
     CDS             1741..2691
                     /codon_start=1
                     /label=hSOX2
                     /note="Homo sapiens transcription factor SOX-2 gene.
                     Belongs to the SRY-related HMG-box (SOX) family of 
                     transcription factors, which is involved in the regulation 
                     of embryonic development and in the determination of cell 
                     fate"
                     /translation="MYNMMETELKPPGPQQTSGGGGGNSTAAAAGGNQKNSPDRVKRPM
                     NAFMVWSRGQRRKMAQENPKMHNSEISKRLGAEWKLLSETEKRPFIDEAKRLRALHMKE
                     HPDYKYRPRRKTKTLMKKDKYTLPGGLLAPGGNSMASGVGVGAGLGAGVNQRMDSYAHM
                     NGWSNGSYSMMQDQLGYPQHPGLNAHGAAQMQPMHRYDVSALQYNSMTSSQTYMNGSPT
                     YSMSYSQQGTPGMALGSMGSVVKSEASSSPPVVTSSSHSRAPCQAGDLRDMISMYLPGA
                     EVPEPAAPSRLHMSQHYQSGPVPGTAINGTLPLSHM"
     CDS             2716..2781
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             2806..4215
                     /codon_start=1
                     /label=hKLF4
                     /note="Homo sapiens Kruppel-like factor 4 (Klf4) gene.
                     Belongs to the relatively large family of SP1-like 
                     transcription factors and is involved in the regulation of 
                     proliferation, differentiation, apoptosis and somatic cell 
                     reprogramming."
                     /translation="MAVSDALLPSFSTFASGPAGREKTLRQAGAPNNRWREELSHMKRL
                     PPVLPGRPYDLAAATVATDLESGGAGAACGGSNLAPLPRRETEEFNDLLDLDFILSNSL
                     THPPESVAATVSSSASASSSSSPSSSGPASAPSTCSFTYPIRAGNDPGVAPGGTGGGLL
                     YGRESAPPPTAPFNLADINDVSPSGGFVAELLRPELDPVYIPPQQPQPPGGGLMGKFVL
                     KASLSAPGSEYGSPSVISVSKGSPDGSHPVVVAPYNGGPPRTCPKIKQEAVSSCTHLGA
                     GPPLSNGHRPAAHDFPLGRQLPSRTTPTLGLEEVLSSRDCHPALPLPPGFHPHPGPNYP
                     SFLPDQMQPQVPPLHYQELMPPGSCMPEEPKPKRGRRSWPRKRTATHTCDYAGCGKTYT
                     KSSHLKAHLRTHTGEKPYHCDWDGCGWKFARSDELTRHYRKHTGHRPFQCQKCDRAFSR
                     SDHLALHMKRHF"
     primer_bind     complement(4321..4340)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4386..4441
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4440..4459)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also
                     called rb-glob-pA-term-R"
     primer_bind     complement(4802..4818)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4802..4818)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4815..4837)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4826..4842
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4850..4880)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4895..4916)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             5395..7317
                     /codon_start=1
                     /label=EBNA1
                     /note="Epstein-Barr nuclear antigen 1, also known as
                     EBNA-1"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGXGXGGGAGGAGXGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     rep_origin      7622..9411
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(9952..9969)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10123..10711)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10882..11742)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11505..11524
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11743..11847)
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggcggg gggggggggg gggcgcgcgc caggcggggc ggggcggggc gaggggcggg
      541 gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc
      601 cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg
      661 gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc
      721 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc
      781 cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa
      841 gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct
      961 gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg
     1021 gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt
     1081 gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc
     1141 cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg
     1201 gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg
     1261 ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg agcgccggcg
     1321 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc
     1381 agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc
     1441 cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg
     1501 gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc
     1561 ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga
     1621 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc
     1681 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattc gcccttcacc
     1741 atgtacaaca tgatggagac ggagctgaag ccgccgggcc cgcagcaaac ttcggggggc
     1801 ggcggcggca actccaccgc ggcggcggcc ggcggcaacc agaaaaacag cccggaccgc
     1861 gtcaagcggc ccatgaatgc cttcatggtg tggtcccgcg ggcagcggcg caagatggcc
     1921 caggagaacc ccaagatgca caactcggag atcagcaagc gcctgggcgc cgagtggaaa
     1981 cttttgtcgg agacggagaa gcggccgttc atcgacgagg ctaagcggct gcgagcgctg
     2041 cacatgaagg agcacccgga ttataaatac cggccccggc ggaaaaccaa gacgctcatg
     2101 aagaaggata agtacacgct gcccggcggg ctgctggccc ccggcggcaa tagcatggcg
     2161 agcggggtcg gggtgggcgc cggcctgggc gcgggcgtga accagcgcat ggacagttac
     2221 gcgcacatga acggctggag caacggcagc tacagcatga tgcaggacca gctgggctac
     2281 ccgcagcacc cgggcctcaa tgcgcacggc gcagcgcaga tgcagcccat gcaccgctac
     2341 gacgtgagcg ccctgcagta caactccatg accagctcgc agacctacat gaacggctcg
     2401 cccacctaca gcatgtccta ctcgcagcag ggcacccctg gcatggctct tggctccatg
     2461 ggttcggtgg tcaagtccga ggccagctcc agcccccctg tggttacctc ttcctcccac
     2521 tccagggcgc cctgccaggc cggggacctc cgggacatga tcagcatgta tctccccggc
     2581 gccgaggtgc cggaacccgc cgcccccagc agacttcaca tgtcccagca ctaccagagc
     2641 ggcccggtgc ccggcacggc cattaacggc acactgcccc tctcacacat gggggatctc
     2701 aaaattgtcg ctcctgtcaa acaaactctt aactttgatt tactcaaact ggctggggat
     2761 gtagaaagca atccaggtcc actcatgaac aattcgccct tcaccatggc tgtcagtgac
     2821 gcgctgctcc catctttctc cacgttcgcg tctggcccgg cgggaaggga gaagacactg
     2881 cgtcaagcag gtgccccgaa taaccgctgg cgggaggagc tctcccacat gaagcgactt
     2941 cccccagtgc ttcccggccg cccctatgac ctggcggcgg cgaccgtggc cacagacctg
     3001 gagagcggcg gagccggtgc ggcttgcggc ggtagcaacc tggcgcccct acctcggaga
     3061 gagaccgagg agttcaacga tctcctggac ctggacttta ttctctccaa ttcgctgacc
     3121 catcctccgg agtcagtggc cgccaccgtg tcctcgtcag cgtcagcctc ctcttcgtcg
     3181 tcgccgtcga gcagcggccc tgccagcgcg ccctccacct gcagcttcac ctatccgatc
     3241 cgggccggga acgacccggg cgtggcgccg ggcggcacgg gcggaggcct cctctatggc
     3301 agggagtccg ctccccctcc gacggctccc ttcaacctgg cggacatcaa cgacgtgagc
     3361 ccctcgggcg gcttcgtggc cgagctcctg cggccagaat tggacccggt gtacattccg
     3421 ccgcagcagc cgcagccgcc aggtggcggg ctgatgggca agttcgtgct gaaggcgtcg
     3481 ctgagcgccc ctggcagcga gtacggcagc ccgtcggtca tcagcgtcag caaaggcagc
     3541 cctgacggca gccacccggt ggtggtggcg ccctacaacg gcgggccgcc gcgcacgtgc
     3601 cccaagatca agcaggaggc ggtctcttcg tgcacccact tgggcgctgg accccctctc
     3661 agcaatggcc accggccggc tgcacacgac ttccccctgg ggcggcagct ccccagcagg
     3721 actaccccga ccctgggtct tgaggaagtg ctgagcagca gggactgtca ccctgccctg
     3781 ccgcttcctc ccggcttcca tccccacccg gggcccaatt acccatcctt cctgcccgat
     3841 cagatgcagc cgcaagtccc gccgctccat taccaagagc tcatgccacc cggttcctgc
     3901 atgccagagg agcccaagcc aaagagggga agacgatcgt ggccccggaa aaggaccgcc
     3961 acccacactt gtgattacgc gggctgcggc aaaacctaca caaagagttc ccatctcaag
     4021 gcacacctgc gaacccacac aggtgagaaa ccttaccact gtgactggga cggctgtgga
     4081 tggaaattcg cccgctcaga tgaactgacc aggcactacc gtaaacacac ggggcaccgc
     4141 ccgttccagt gccaaaaatg cgaccgagca ttttccaggt cggaccacct cgccttacac
     4201 atgaagagac atttttaaaa gggcgaattg cctgcaggaa ttcactcctc aggtgcaggc
     4261 tgcctatcag aaggtggtgg ctggtgtggc caatgccctg gctcacaaat accactgaga
     4321 tctttttccc tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc
     4381 tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc
     4441 actcggaagg acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta
     4501 gagtttggca acatatgccc atatgctggc tgccatgaac aaaggttggc tataaagagg
     4561 tcatcagtat atgaaacagc cccctgctgt ccattcctta ttccatagaa aagccttgac
     4621 ttgaggttag atttttttta tattttgttt tgtgttattt ttttctttaa catccctaaa
     4681 attttcctta catgttttac tagccagatt tttcctcctc tcctgactac tcccagtcat
     4741 agctgtccct cttctcttat ggagatccct cgacctgcag cccaagcttg gcgtaatcat
     4801 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     4861 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     4921 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg atctcaattc
     4981 tcatgtttga cagcttatca tcgataagct gatcctcaca ggccgcaccc agcttttctt
     5041 ccgttgcccc agtagcatct ctgtctggtg accttgaaga ggaagaggag gggtcccgag
     5101 aatccccatc cctaccgtcc agcaaaaagg gggacgagga atttgaggcc tggcttgagg
     5161 ctcaggacgc aaatcttgag gatgttcagc gggagttttc cgggctgcga gtaattggtg
     5221 atgaggacga ggatggttcg gaggatgggg aattttcaga cctggatctg tctgacagcg
     5281 accatgaagg ggatgagggt gggggggctg ttggaggggg caggagtctg cactccctgt
     5341 attcactgag cgtcgtctaa taaagatgtc tattgatctc ttttagtgtg aatcatgtct
     5401 gacgaggggc caggtacagg acctggaaat ggcctaggag agaagggaga cacatctgga
     5461 ccagaaggct ccggcggcag tggacctcaa agaagagggg gtgataacca tggacgagga
     5521 cggggaagag gacgaggacg aggaggcgga agaccaggag ccccgggcgg ctcaggatca
     5581 gggccaagac atagagatgg tgtccggaga ccccaaaaac gtccaagttg cattggctgc
     5641 aaagggaccc acggtggaac aggagcagga gcaggagcgg gaggggcagg agcaggaggg
     5701 gcaggagcag gaggaggggc aggagcagga ggaggggcag gaggggcagg aggggcagga
     5761 ggggcaggag caggaggagg ggcaggagca ggaggagggg caggaggggc aggaggggca
     5821 ggagcaggag gaggggsagg agsaggagga ggggcaggag gggcaggags aggaggaggg
     5881 gcaggagggg caggaggggc aggagcagga ggaggggcag gagcaggagg aggggcagga
     5941 ggggcaggag caggaggagg ggcaggaggg gcaggagggg caggagcagg aggaggggca
     6001 ggagcaggag gggcaggagg ggcaggaggg gcaggagcag gaggggcagg agcaggagga
     6061 ggggcaggag gggcaggagg ggcaggagca ggaggggcag gagcaggagg ggcaggagca
     6121 ggaggggcag gagcaggagg ggcaggaggg gcaggagcag gaggggcagg aggggcagga
     6181 gcaggagggg caggaggggc aggagcagga ggaggggcag gaggggcagg agcaggagga
     6241 ggggcaggag gggcaggagc aggaggggca ggaggggcag gagcaggagg ggcaggaggg
     6301 gcaggagcag gaggggcagg aggggcagga gcaggaggag gggcaggagc aggaggggca
     6361 ggagcaggag gtggaggccg gggtcgagga ggcagtggag gccggggtcg aggaggtagt
     6421 ggaggccggg gtcgaggagg tagtggaggc cgccggggta gaggacgtga aagagccagg
     6481 gggggaagtc gtgaaagagc cagggggaga ggtcgtggac gtggagaaaa gaggcccagg
     6541 agtcccagta gtcagtcatc atcatccggg tctccaccgc gcaggccccc tccaggtaga
     6601 aggccatttt tccaccctgt aggggaagcc gattattttg aataccacca agaaggtggc
     6661 ccagatggtg agcctgacgt gcccccggga gcgatagagc agggccccgc agatgaccca
     6721 ggagaaggcc caagcactgg accccggggt cagggtgatg gaggcaggcg caaaaaagga
     6781 gggtggtttg gaaagcatcg tggtcaagga ggttccaacc cgaaatttga gaacattgca
     6841 gaaggtttaa gagctctcct ggctaggagt cacgtagaaa ggactaccga cgaaggaact
     6901 tgggtcgccg gtgtgttcgt atatggaggt agtaagacct ccctttacaa cctaaggcga
     6961 ggaactgccc ttgctattcc acaatgtcgt cttacaccat tgagtcgtct cccctttgga
     7021 atggcccctg gacccggccc acaacctggc ccgctaaggg agtccattgt ctgttatttc
     7081 atggtctttt tacaaactca tatatttgct gaggttttga aggatgcgat taaggacctt
     7141 gttatgacaa agcccgctcc tacctgcaat atcagggtga ctgtgtgcag ctttgacgat
     7201 ggagtagatt tgcctccctg gtttccacct atggtggaag gggctgccgc ggagggtgat
     7261 gacggagatg acggagatga aggaggtgat ggagatgagg gtgaggaagg gcaggagtga
     7321 tgtaacttgt taggagacgc cctcaatcgt attaaaagcc gtgtattccc ccgcactaaa
     7381 gaataaatcc ccagtagaca tcatgcgtgc tgttggtgta tttctggcca tctgtcttgt
     7441 caccattttc gtcctcccaa catggggcaa ttgggcatac ccatgttgtc acgtcactca
     7501 gctccgcgct caacaccttc tcgcgttgga aaacattagc gacatttacc tggtgagcaa
     7561 tcagacatgc gacggcttta gcctggcctc cttaaattca cctaagaatg ggagcaacca
     7621 gcaggaaaag gacaagcagc gaaaattcac gcccccttgg gaggtggcgg catatgcaaa
     7681 ggatagcact cccactctac tactgggtat catatgctga ctgtatatgc atgaggatag
     7741 catatgctac ccggatacag attaggatag catatactac ccagatatag attaggatag
     7801 catatgctac ccagatatag attaggatag cctatgctac ccagatataa attaggatag
     7861 catatactac ccagatatag attaggatag catatgctac ccagatatag attaggatag
     7921 cctatgctac ccagatatag attaggatag catatgctac ccagatatag attaggatag
     7981 catatgctat ccagatattt gggtagtata tgctacccag atataaatta ggatagcata
     8041 tactacccta atctctatta ggatagcata tgctacccgg atacagatta ggatagcata
     8101 tactacccag atatagatta ggatagcata tgctacccag atatagatta ggatagccta
     8161 tgctacccag atataaatta ggatagcata tactacccag atatagatta ggatagcata
     8221 tgctacccag atatagatta ggatagccta tgctacccag atatagatta ggatagcata
     8281 tgctatccag atatttgggt agtatatgct acccatggca acattagccc accgtgctct
     8341 cagcgacctc gtgaatatga ggaccaacaa ccctgtgctt ggcgctcagg cgcaagtgtg
     8401 tgtaatttgt cctccagatc gcagcaatcg cgcccctatc ttggcccgcc cacctactta
     8461 tgcaggtatt ccccggggtg ccattagtgg ttttgtgggc aagtggtttg accgcagtgg
     8521 ttagcggggt tacaatcagc caagttatta cacccttatt ttacagtcca aaaccgcagg
     8581 gcggcgtgtg ggggctgacg cgtgccccca ctccacaatt tcaaaaaaaa gagtggccac
     8641 ttgtctttgt ttatgggccc cattggcgtg gagccccgtt taattttcgg gggtgttaga
     8701 gacaaccagt ggagtccgct gctgtcggcg tccactctct ttccccttgt tacaaataga
     8761 gtgtaacaac atggttcacc tgtcttggtc cctgcctggg acacatctta ataaccccag
     8821 tatcatattg cactaggatt atgtgttgcc catagccata aattcgtgtg agatggacat
     8881 ccagtcttta cggcttgtcc ccaccccatg gatttctatt gttaaagata ttcagaatgt
     8941 ttcattccta cactagtatt tattgcccaa ggggtttgtg agggttatat tggtgtcata
     9001 gcacaatgcc accactgaac cccccgtcca aattttattc tgggggcgtc acctgaaacc
     9061 ttgttttcga gcacctcaca tacaccttac tgttcacaac tcagcagtta ttctattagc
     9121 taaacgaagg agaatgaaga agcaggcgaa gattcaggag agttcactgc ccgctccttg
     9181 atcttcagcc actgcccttg tgactaaaat ggttcactac cctcgtggaa tcctgacccc
     9241 atgtaaataa aaccgtgaca gctcatgggg tgggagatat cgctgttcct taggaccctt
     9301 ttactaaccc taattcgata gcatatgctt cccgttgggt aacatatgct attgaattag
     9361 ggttagtctg gatagtatat actactaccc gggaagcata tgctacccgt ttagggttaa
     9421 caagggggcc ttataaacac tattgctaat gccctcttga gggtccgctt atcggtagct
     9481 acacaggccc ctctgattga cgttggtgta gcctcccgta gtcttcctgg gcccctggga
     9541 ggtacatgtc ccccagcatt ggtgtaagag cttcagccaa gagttacaca taaaggcaat
     9601 gttgtgttgc agtccacaga ctgcaaagtc tgctccagga tgaaagccac tcagtgttgg
     9661 caaatgtgca catccattta taaggatgtc aactacagtc agagaacccc tttgtgtttg
     9721 gtcccccccc gtgtcacatg tggaacaggg cccagttggc aagttgtacc aaccaactga
     9781 agggattaca tgcactgccc cgcgaagaag gggcagagat gtcgtagtca ggtttagttc
     9841 gtccggggcg gggatcgatc ctctagagtc gacgctagcg gatccgctgc attaatgaat
     9901 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     9961 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
    10021 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
    10081 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
    10141 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
    10201 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
    10261 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
    10321 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
    10381 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
    10441 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
    10501 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
    10561 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
    10621 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
    10681 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
    10741 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
    10801 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
    10861 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
    10921 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
    10981 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
    11041 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    11101 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    11161 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    11221 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    11281 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    11341 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    11401 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    11461 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    11521 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    11581 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    11641 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    11701 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    11761 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    11821 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgggtc
//
