LOCUS       V046022                 8083 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V046022
VERSION     V046022
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     protein_bind    820..838
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    841..859
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     CDS             990..3905
                     /label="CSF1R(NM_001288705)"
                     /note="CSF1R(NM_001288705)"
                     /gene="CSF1R"
     polyA_signal    4041..4265
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     protein_bind    4549..4596
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2μ plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core
                     sequence TCTAGAAA (Turan and Bode, 2011)."
                     /label="FRT"
     CDS             4604..5626
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /note="confers resistance to hygromycin"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /transl_table=1
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     *"
                     /label="HygR"
     polyA_signal    5756..5877
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5926..5942)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5950..5966
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5974..6004)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6019..6040
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6328..6916)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7087..7947)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7948..8052)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttgccacca tgggcccagg agttctgctg ctcctgctgg
     1021 tggccacagc ttggcatggt cagggaatcc cagtgataga gcccagtgtc cctgagctgg
     1081 tcgtgaagcc aggagcaacg gtgaccttgc gatgtgtggg caatggcagc gtggaatggg
     1141 atggcccccc atcacctcac tggaccctgt actctgatgg ctccagcagc atcctcagca
     1201 ccaacaacgc taccttccaa aacacgggga cctatcgctg cactgagcct ggagaccccc
     1261 tgggaggcag cgccgccatc cacctctatg tcaaagaccc tgcccggccc tggaacgtgc
     1321 tagcacagga ggtggtcgtg ttcgaggacc aggacgcact actgccctgt ctgctcacag
     1381 acccggtgct ggaagcaggc gtctcgctgg tgcgtgtgcg tggccggccc ctcatgcgcc
     1441 acaccaacta ctccttctcg ccctggcatg gcttcaccat ccacagggcc aagttcattc
     1501 agagccagga ctatcaatgc agtgccctga tgggtggcag gaaggtgatg tccatcagca
     1561 tccggctgaa agtgcagaaa gtcatcccag ggcccccagc cttgacactg gtgcctgcag
     1621 agctggtgcg gattcgaggg gaggctgccc agatcgtgtg ctcagccagc agcgttgatg
     1681 ttaactttga tgtcttcctc caacacaaca acaccaagct cgcaatccct caacaatctg
     1741 actttcataa taaccgttac caaaaagtcc tgaccctcaa cctcgatcaa gtagatttcc
     1801 aacatgccgg caactactcc tgcgtggcca gcaacgtgca gggcaagcac tccacctcca
     1861 tgttcttccg ggtggtagag agtgcctact tgaacttgag ctctgagcag aacctcatcc
     1921 aggaggtgac cgtgggggag gggctcaacc tcaaagtcat ggtggaggcc tacccaggcc
     1981 tgcaaggttt taactggacc tacctgggac ccttttctga ccaccagcct gagcccaagc
     2041 ttgctaatgc taccaccaag gacacataca ggcacacctt caccctctct ctgccccgcc
     2101 tgaagccctc tgaggctggc cgctactcct tcctggccag aaacccagga ggctggagag
     2161 ctctgacgtt tgagctcacc cttcgatacc ccccagaggt aagcgtcata tggacattca
     2221 tcaacggctc tggcaccctt ttgtgtgctg cctctgggta cccccagccc aacgtgacat
     2281 ggctgcagtg cagtggccac actgataggt gtgatgaggc ccaagtgctg caggtctggg
     2341 atgacccata ccctgaggtc ctgagccagg agcccttcca caaggtgacg gtgcagagcc
     2401 tgctgactgt tgagacctta gagcacaacc aaacctacga gtgcagggcc cacaacagcg
     2461 tggggagtgg ctcctgggcc ttcataccca tctctgcagg agcccacacg catcccccgg
     2521 atgagttcct cttcacacca gtggtggtcg cctgcatgtc catcatggcc ttgctgctgc
     2581 tgctgctcct gctgctattg tacaagtata agcagaagcc caagtaccag gtccgctgga
     2641 agatcatcga gagctatgag ggcaacagtt atactttcat cgaccccacg cagctgcctt
     2701 acaacgagaa gtgggagttc ccccggaaca acctgcagtt tggtaagacc ctcggagctg
     2761 gagcctttgg gaaggtggtg gaggccacgg cctttggtct gggcaaggag gatgctgtcc
     2821 tgaaggtggc tgtgaagatg ctgaagtcca cggcccatgc tgatgagaag gaggccctca
     2881 tgtccgagct gaagatcatg agccacctgg gccagcacga gaacatcgtc aaccttctgg
     2941 gagcctgtac ccatggaggc cctgtactgg tcatcacgga gtactgttgc tatggcgacc
     3001 tgctcaactt tctgcgaagg aaggctgagg ccatgctggg acccagcctg agccccggcc
     3061 aggaccccga gggaggcgtc gactataaga acatccacct cgagaagaaa tatgtccgca
     3121 gggacagtgg cttctccagc cagggtgtgg acacctatgt ggagatgagg cctgtctcca
     3181 cttcttcaaa tgactccttc tctgagcaag acctggacaa ggaggatgga cggcccctgg
     3241 agctccggga cctgcttcac ttctccagcc aagtagccca gggcatggcc ttcctcgctt
     3301 ccaagaattg catccaccgg gacgtggcag cgcgtaacgt gctgttgacc aatggtcatg
     3361 tggccaagat tggggacttc gggctggcta gggacatcat gaatgactcc aactacattg
     3421 tcaagggcaa tgcccgcctg cctgtgaagt ggatggcccc agagagcatc tttgactgtg
     3481 tctacacggt tcagagcgac gtctggtcct atggcatcct cctctgggag atcttctcac
     3541 ttgggctgaa tccctaccct ggcatcctgg tgaacagcaa gttctataaa ctggtgaagg
     3601 atggatacca aatggcccag cctgcatttg ccccaaagaa tatatacagc atcatgcagg
     3661 cctgctgggc cttggagccc acccacagac ccaccttcca gcagatctgc tccttccttc
     3721 aggagcaggc ccaagaggac aggagagagc gggactatac caatctgccg agcagcagca
     3781 gaagcggtgg cagcggcagc agcagcagtg agctggagga ggagagctct agtgagcacc
     3841 tgacctgctg cgagcaaggg gatatcgccc agcccttgct gcagcccaac aactatcagt
     3901 tctgcgtgag cggctggcgg ctgttcaaga agattagctg aggatccact agtccagtgt
     3961 ggtggaattc tgcagatatc cagcacagtg gcggccgctc gagtctagag ggcccgttta
     4021 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     4081 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     4141 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     4201 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     4261 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg
     4321 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     4381 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     4441 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     4501 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtacctaga agttcctatt
     4561 ccgaagttcc tattctctag aaagtatagg aacttccttg gccaaaaagc ctgaactcac
     4621 cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca
     4681 gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt
     4741 cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt
     4801 tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct
     4861 gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga
     4921 actgcccgct gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct
     4981 tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg
     5041 gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga
     5101 cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga
     5161 ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga
     5221 caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata
     5281 cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg
     5341 ctacttcgag cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct
     5401 ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc
     5461 ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac
     5521 acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga
     5581 tagtggaaac cgacgcccca gcactcgtcc gagggcaaag gaatagcacg tactacgaga
     5641 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     5701 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
     5761 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     5821 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     5881 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     5941 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     6001 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     6061 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     6121 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     6181 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     6241 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     6301 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     6361 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     6421 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     6481 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     6541 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     6601 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     6661 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     6721 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     6781 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     6841 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     6901 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     6961 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     7021 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     7081 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     7141 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     7201 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     7261 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     7321 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     7381 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     7441 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     7501 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     7561 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     7621 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     7681 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     7741 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     7801 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     7861 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     7921 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     7981 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     8041 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtc
//
