LOCUS       V045760                 6376 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V045760
VERSION     V045760
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     protein_bind    820..838
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    841..859
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     CDS             990..2198
                     /label="AURKA(NM_198437)"
                     /note="AURKA(NM_198437)"
                     /gene="AURKA"
     polyA_signal    2334..2558
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     protein_bind    2842..2889
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2μ plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core
                     sequence TCTAGAAA (Turan and Bode, 2011)."
                     /label="FRT"
     CDS             2897..3919
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /note="confers resistance to hygromycin"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /transl_table=1
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     *"
                     /label="HygR"
     polyA_signal    4049..4170
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(4219..4235)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    4243..4259
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(4267..4297)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    4312..4333
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(4621..5209)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(5380..6240)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(6241..6345)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttgccacca tggaccgatc taaagaaaac tgcatttcag
     1021 gacctgttaa ggctacagct ccagttggag gtccaaaacg tgttctcgtg actcagcaat
     1081 ttccttgtca gaatccatta cctgtaaata gtggccaggc tcagcgggtc ttgtgtcctt
     1141 caaattcttc ccagcgcatt cctttgcaag cacaaaagct tgtctccagt cacaagccgg
     1201 ttcagaatca gaagcagaag caattgcagg caaccagtgt acctcatcct gtctccaggc
     1261 cactgaataa cacccaaaag agcaagcagc ccctgccatc ggcacctgaa aataatcctg
     1321 aggaggaact ggcatcaaaa cagaaaaatg aagaatcaaa aaagaggcag tgggctttgg
     1381 aagactttga aattggtcgc cctctgggta aaggaaagtt tggtaatgtt tatttggcaa
     1441 gagaaaagca aagcaagttt attctggctc ttaaagtgtt atttaaagct cagctggaga
     1501 aagccggagt ggagcatcag ctcagaagag aagtagaaat acagtcccac cttcggcatc
     1561 ctaatattct tagactgtat ggttatttcc atgatgctac cagagtctac ctaattctgg
     1621 aatatgcacc acttggaaca gtttatagag aacttcagaa actttcaaag tttgatgagc
     1681 agagaactgc tacttatata acagaattgg caaatgccct gtcttactgt cattcgaaga
     1741 gagttattca tagagacatt aagccagaga acttacttct tggatcagct ggagagctta
     1801 aaattgcaga ttttgggtgg tcagtacatg ctccatcttc caggaggacc actctctgtg
     1861 gcaccctgga ctacctgccc cctgaaatga ttgaaggtcg gatgcatgat gagaaggtgg
     1921 atctctggag ccttggagtt ctttgctatg aatttttagt tgggaagcct ccttttgagg
     1981 caaacacata ccaagagacc tacaaaagaa tatcacgggt tgaattcaca ttccctgact
     2041 ttgtaacaga gggagccagg gacctcattt caagactgtt gaagcataat cccagccaga
     2101 ggccaatgct cagagaagta cttgaacacc cctggatcac agcaaattca tcaaaaccat
     2161 caaattgcca aaacaaagaa tcagctagca aacagtctgt gagcggctgg cggctgttca
     2221 agaagattag ctgaggatcc actagtccag tgtggtggaa ttctgcagat atccagcaca
     2281 gtggcggccg ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
     2341 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     2401 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     2461 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     2521 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     2581 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     2641 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     2701 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     2761 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     2821 gggtgatggt tcacgtacct agaagttcct attccgaagt tcctattctc tagaaagtat
     2881 aggaacttcc ttggccaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat
     2941 cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc
     3001 tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg
     3061 tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga
     3121 agtgcttgac attggggaat tcagcgagag cctgacctat tgcatctccc gccgtgcaca
     3181 gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc agccggtcgc
     3241 ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt
     3301 cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga
     3361 tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca
     3421 ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca
     3481 cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga
     3541 ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag
     3601 gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct
     3661 tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc aactctatca
     3721 gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat
     3781 cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt
     3841 ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg
     3901 tccgagggca aaggaatagc acgtactacg agatttcgat tccaccgccg ccttctatga
     3961 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     4021 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
     4081 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     4141 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
     4201 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     4261 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     4321 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     4381 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     4441 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     4501 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     4561 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     4621 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     4681 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     4741 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     4801 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     4861 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     4921 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     4981 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     5041 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     5101 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     5161 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     5221 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     5281 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     5341 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     5401 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     5461 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     5521 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     5581 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     5641 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     5701 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     5761 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     5821 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     5881 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     5941 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     6001 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     6061 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     6121 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     6181 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     6241 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     6301 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     6361 agtgccacct gacgtc
//
