LOCUS V045760 6376 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V045760 VERSION V045760 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" protein_bind 820..838 /bound_moiety="tetracycline repressor TetR" /gene="tetO" /note="bacterial operator O2 for the tetR and tetA genes" /label="tet operator" protein_bind 841..859 /bound_moiety="tetracycline repressor TetR" /gene="tetO" /note="bacterial operator O2 for the tetR and tetA genes" /label="tet operator" CDS 990..2198 /label="AURKA(NM_198437)" /note="AURKA(NM_198437)" /gene="AURKA" polyA_signal 2334..2558 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" protein_bind 2842..2889 /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2μ plasmid" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." /label="FRT" CDS 2897..3919 /codon_start=1 /gene="aph(4)-Ia" /note="confers resistance to hygromycin" /product="aminoglycoside phosphotransferase from E. coli" /transl_table=1 /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE *" /label="HygR" polyA_signal 4049..4170 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(4219..4235) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4243..4259 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4267..4297) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4312..4333 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(4621..5209) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5380..6240) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6241..6345) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc 841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga 901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga 961 ctctagcgtt taaacttaag cttgccacca tggaccgatc taaagaaaac tgcatttcag 1021 gacctgttaa ggctacagct ccagttggag gtccaaaacg tgttctcgtg actcagcaat 1081 ttccttgtca gaatccatta cctgtaaata gtggccaggc tcagcgggtc ttgtgtcctt 1141 caaattcttc ccagcgcatt cctttgcaag cacaaaagct tgtctccagt cacaagccgg 1201 ttcagaatca gaagcagaag caattgcagg caaccagtgt acctcatcct gtctccaggc 1261 cactgaataa cacccaaaag agcaagcagc ccctgccatc ggcacctgaa aataatcctg 1321 aggaggaact ggcatcaaaa cagaaaaatg aagaatcaaa aaagaggcag tgggctttgg 1381 aagactttga aattggtcgc cctctgggta aaggaaagtt tggtaatgtt tatttggcaa 1441 gagaaaagca aagcaagttt attctggctc ttaaagtgtt atttaaagct cagctggaga 1501 aagccggagt ggagcatcag ctcagaagag aagtagaaat acagtcccac cttcggcatc 1561 ctaatattct tagactgtat ggttatttcc atgatgctac cagagtctac ctaattctgg 1621 aatatgcacc acttggaaca gtttatagag aacttcagaa actttcaaag tttgatgagc 1681 agagaactgc tacttatata acagaattgg caaatgccct gtcttactgt cattcgaaga 1741 gagttattca tagagacatt aagccagaga acttacttct tggatcagct ggagagctta 1801 aaattgcaga ttttgggtgg tcagtacatg ctccatcttc caggaggacc actctctgtg 1861 gcaccctgga ctacctgccc cctgaaatga ttgaaggtcg gatgcatgat gagaaggtgg 1921 atctctggag ccttggagtt ctttgctatg aatttttagt tgggaagcct ccttttgagg 1981 caaacacata ccaagagacc tacaaaagaa tatcacgggt tgaattcaca ttccctgact 2041 ttgtaacaga gggagccagg gacctcattt caagactgtt gaagcataat cccagccaga 2101 ggccaatgct cagagaagta cttgaacacc cctggatcac agcaaattca tcaaaaccat 2161 caaattgcca aaacaaagaa tcagctagca aacagtctgt gagcggctgg cggctgttca 2221 agaagattag ctgaggatcc actagtccag tgtggtggaa ttctgcagat atccagcaca 2281 gtggcggccg ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc 2341 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg 2401 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag 2461 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga 2521 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag 2581 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt 2641 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc 2701 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg 2761 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta 2821 gggtgatggt tcacgtacct agaagttcct attccgaagt tcctattctc tagaaagtat 2881 aggaacttcc ttggccaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat 2941 cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc 3001 tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg 3061 tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga 3121 agtgcttgac attggggaat tcagcgagag cctgacctat tgcatctccc gccgtgcaca 3181 gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc agccggtcgc 3241 ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt 3301 cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga 3361 tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca 3421 ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca 3481 cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga 3541 ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag 3601 gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct 3661 tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc aactctatca 3721 gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat 3781 cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt 3841 ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg 3901 tccgagggca aaggaatagc acgtactacg agatttcgat tccaccgccg ccttctatga 3961 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga 4021 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa 4081 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg 4141 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta 4201 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat 4261 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag 4321 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg 4381 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc 4441 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 4501 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 4561 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 4621 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 4681 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 4741 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 4801 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 4861 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 4921 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 4981 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 5041 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac 5101 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 5161 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 5221 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 5281 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 5341 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 5401 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 5461 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 5521 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 5581 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 5641 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 5701 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 5761 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 5821 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 5881 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 5941 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 6001 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 6061 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 6121 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 6181 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 6241 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 6301 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 6361 agtgccacct gacgtc //