LOCUS       V037251                12162 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V037251
VERSION     V037251
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1494..1538
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1801..1916
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        1974..2277
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2278..2481
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2588..6232
                     /label="Zeb2(NM_015753)"
                     /note="Zeb2(NM_015753)"
                     /gene="Zeb2"
     CDS             6257..6328
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags"
                     /transl_table=1
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
                     /label="3xFLAG"
     promoter        6382..6593
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6606..6874
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6902..7594
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="Zoanthus green fluorescent protein"
                     /transl_table=1
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
                     LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
                     CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
                     DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
                     AIASGSALP"
                     /label="ZsGreen1"
     CDS             7604..7657
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7658..8257
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8258..8845
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8919..9152
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9224..9345
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9364..9499
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9537..9553)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9561..9577
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9585..9615)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9630..9651
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9939..10527)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10698..11558)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11559..11663)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     12137..12153
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     1441 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     1501 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     1561 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     1621 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     1681 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     1741 gtggagagag agacagagac agatccattc gattagtgaa cggatctcga cggtatcgcc
     1801 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     1861 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg
     1921 gtttattaca gggacagcag agatccagtt tatcgataag cttgggagtt ccgcgttaca
     1981 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     2041 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     2101 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     2161 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     2221 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
     2281 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
     2341 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
     2401 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
     2461 gaggtctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca
     2521 cgctgttttg acctccatag aagacaccga ctctactaga ggatctattt ccggtgaatt
     2581 cgccaccatg aagcagccga tcatggcgga tggcccccgg tgcaagaggc gcaaacaagc
     2641 caatcccagg aggaaaaacg tggtgaacta tgacaacgta gtggacgcag gctcggagac
     2701 agatgaagag gacaagcttc acattgctga agatgatagc cttgcaaacc ctctggacca
     2761 ggacaccagc ccagctagca tgcccaacca tgagtcctcc ccacacatga gccaagggct
     2821 gctaccaaga gaggaagaag aggaggagct aagggagagt gttgtggagc acagctggca
     2881 cagtggcgag attctgcaag cctctgtagc cggtccagaa gaaatgaagg aagattatga
     2941 tgcaatgggg ccagaagcca cgatccagac cacaattaac aatggtacag ttaagaatgc
     3001 aaattgcaca tcagactttg aggaatactt tgccaaaaga aaactggagg aaagagatgg
     3061 ccacgcagtg agcatcgaag agtaccttca gcgaagcgac acggccatta tttacccaga
     3121 agcccctgag gagctgtctc gccttggcac gccagaggcc aacgggcaag aagaaaatga
     3181 cctgccacct ggaactccag atgcttttgc ccaactgctg acctgcccct actgcgaccg
     3241 cggctacaag cgcttgacat cgctgaagga gcacatcaag taccgccacg agaagaatga
     3301 agagaacttt tcctgccctc tttgtagcta cacgttcgcc taccgcaccc agctcgagag
     3361 gcatatggtg acgcacaagc cagggacaga tcagcaccaa atgctaaccc aaggagcagg
     3421 taaccgcaag ttcaagtgca cggagtgtgg caaggccttc aagtacaagc accacctgaa
     3481 agaacacctg agaattcaca gtggtgaaaa accttacgaa tgcccaaact gcaagaaacg
     3541 cttctctcat tctgggtcct acagttcaca tatcagcagc aagaaatgta ttggtttaat
     3601 atcagtaaat ggccgaatga gaaacaatat caagacgggt tcttccccta attctgtttc
     3661 ttcttctcct actaactcag ccattactca gttaaggaac aagttggaaa atggaaaacc
     3721 acttagcatg tctgagcaga caggcttact taagattaaa acagaaccac tagacttcaa
     3781 tgactataaa gttcttatgg caacacatgg gtttagtggc agcagtccct ttatgaacgg
     3841 tgggcttgga gccaccagcc ctttaggtgt acacccatct gctcagagtc caatgcagca
     3901 cttaggtgta gggatggaag cccctttact tggatttccc actatgaata gtaacttgag
     3961 tgaggtacaa aaggttctac agattgtgga caatacggtt tctaggcaaa agatggactg
     4021 caagacggaa gacatttcaa agttgaaagg ttatcacatg aaggatccat gttctcagcc
     4081 agaagaacaa ggggtaactt ctcccaatat tccccctgtc ggtcttccag tagtgagtca
     4141 taacggtgcc actaaaagta ttattgacta taccttagag aaagtcaatg aagccaaagc
     4201 ttgcctccag agcttgacca ccgactcaag gagacagatc agtaacataa agaaagagaa
     4261 gttgcgtact ttgatagatt tggtcactga tgataaaatg attgagaacc acagcatatc
     4321 cactccattt tcatgccagt tctgtaaaga aagcttcccg ggccctattc ccctgcatca
     4381 gcatgaacga tacctgtgta agatgaatga agagatcaag gcagtcctgc aacctcatga
     4441 aaacatagtc cccaacaaag ctggagtttt tgttgataat aaagccctcc tcttgtcatc
     4501 tgtactttcc gagaaaggac tgacaagccc catcaaccca tacaaggacc acatgtctgt
     4561 actgaaagca tactatgcta tgaacatgga gcccaactct gatgaactgc tgaaaatctc
     4621 cattgctgtg ggccttcctc aggaatttgt gaaggaatgg tttgagcaaa gaaaagtcta
     4681 ccagtattcg aattccaggt caccatcact ggaaaggacc tccaagccgt tagctcccaa
     4741 cagtaacccc accacaaaag actctttgtt acccaggtct cctgtaaaac ctatggactc
     4801 catcacatcg ccatctatag cagaactcca caacagtgtt acgagttgtg atcctcctct
     4861 caggctaaca aaatcttccc atttcaccaa tattaaagca gttgataaac tggaccactc
     4921 gaggagtaat actccttctc ctttaaatct ttcctccaca tcttctaaaa actcccacag
     4981 tagctcgtac actccaaata gcttctcttc cgaggagctg caggctgagc cgttggacct
     5041 gtcattacca aaacaaatga gagaacccaa aggtattata gccacaaaga acaaaacaaa
     5101 agctactagc ataaacttag accacaacag tgtttcttca tcgtctgaga attcagatga
     5161 gcctctgaat ttgactttta tcaagaaaga gttttcaaat tctaataacc tggacaataa
     5221 aagcaacaac cctgtgttcg gcatgaaccc atttagtgcc aagcctttat acacccctct
     5281 tccaccacag agcgcatttc cccctgccac tttcatgcca ccagtccaga ccagcatccc
     5341 cgggctacga ccatacccag gactggatca gatgagcttc ctaccgcata tggcctatac
     5401 ctacccaacg ggagcagcta cctttgctga tatgcagcaa aggaggaaat accagaggaa
     5461 acaaggattt cagggagact tgctggatgg agcacaagac tacatgtcag gcctagatga
     5521 catgacagac tccgattcct gtctgtctcg aaagaagata aagaagacag aaagtggcat
     5581 gtatgcatgt gacttatgtg acaagacatt ccagaaaagc agttcccttc tgcgacataa
     5641 atacgaacac acaggaaaga gaccacacca gtgtcagatt tgtaagaaag cgttcaaaca
     5701 caaacaccac cttatcgagc actcgaggct gcactcgggc gagaagccct atcagtgtga
     5761 caaatgtggc aagcgcttct cacactcggg ctcctactcg cagcacatga atcacaggta
     5821 ctcctactgc aagcgggagg cggaggagcg ggaagcagcc gagcgcgagg cgcgagagaa
     5881 agggcacttg gaacccaccg agctgctgat gaaccgggct tacctgcaga gcatcacccc
     5941 tcaggggtac tctgactcgg aggagaggga gagcatgccg agggatggcg agagcgagaa
     6001 ggagcacgag aaggagggcg aggagggtta tgggaagctg cggagaaggg acggcgacga
     6061 ggaggaagag gaggaagagg aagaaagtga aaataaaagt atggatacgg atcccgaaac
     6121 gatacgggat gaggaagaga ctggggatca ctcgatggac gacagttcag aggatgggaa
     6181 aatggaaacc aaatcagacc acgaggaaga caatatggaa gatggcatgg aaggatcctc
     6241 ggtaccaagc ttaagtgact acaaggatga cgatgacaag gattacaaag acgacgatga
     6301 taaggactat aaggatgatg acgacaaata aagatccatc gatactagta aggatctgcg
     6361 atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     6421 ggggaggggt cggcaattga acgggtgcct agagaaggtg gcgcggggta aactgggaaa
     6481 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     6541 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca cagctgaagc
     6601 ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc tacctgaggc cgccatccac
     6661 gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg
     6721 tctaggtaag tttaaagctc aggtcgagac cgggcctttg tccggcgctc ccttggagcc
     6781 tacctagact cagccggctc tccacgcttt gcctgaccct gcttgctcaa ctctacgtct
     6841 ttgtttcgtt ttctgttctg cgccgttaca gatccaagct gtgaccggcg cctactctag
     6901 catggcccag tccaagcacg gcctgaccaa ggagatgacc atgaagtacc gcatggaggg
     6961 ctgcgtggac ggccacaagt tcgtgatcac cggcgagggc atcggctacc ccttcaaggg
     7021 caagcaggcc atcaacctgt gcgtggtgga gggcggcccc ttgcccttcg ccgaggacat
     7081 cttgtccgcc gccttcatgt acggcaaccg cgtgttcacc gagtaccccc aggacatcgt
     7141 cgactacttc aagaactcct gccccgccgg ctacacctgg gaccgctcct tcctgttcga
     7201 ggacggcgcc gtgtgcatct gcaacgccga catcaccgtg agcgtggagg agaactgcat
     7261 gtaccacgag tccaagttct acggcgtgaa cttccccgcc gacggccccg tgatgaagaa
     7321 gatgaccgac aactgggagc cctcctgcga gaagatcatc cccgtgccca agcagggcat
     7381 cttgaagggc gacgtgagca tgtacctgct gctgaaggac ggtggccgct tgcgctgcca
     7441 gttcgacacc gtgtacaagg ccaagtccgt gccccgcaag atgcccgact ggcacttcat
     7501 ccagcacaag ctgacccgcg aggaccgcag cgacgccaag aaccagaagt ggcacctgac
     7561 cgagcacgcc atcgcctccg gctccgcctt gcccgcggcc gctgagggca gaggaagtct
     7621 tctaacatgc ggtgacgtgg aggagaatcc cggccctatg accgagtaca agcccacggt
     7681 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     7741 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     7801 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     7861 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     7921 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     7981 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     8041 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     8101 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     8161 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     8221 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaaat caacctctgg attacaaaat
     8281 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     8341 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     8401 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     8461 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     8521 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     8581 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     8641 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct
     8701 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     8761 cggcctgctg ccggctctgc ggcctcttcc gcgtctccgc cttcgccctc agacgagtcg
     8821 gatctccctt tggccgcctc cccgcctggt acctttaaga ccaatgactt acaaggcagc
     8881 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     8941 acgaaaataa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     9001 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     9061 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     9121 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
     9181 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
     9241 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     9301 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
     9361 gctatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     9421 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     9481 ggaggctttt ttggaggcct agacttttgc agagacggcc caaattcgta atcatggtca
     9541 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     9601 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     9661 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     9721 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     9781 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     9841 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     9901 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     9961 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
    10021 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
    10081 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
    10141 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
    10201 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
    10261 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
    10321 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
    10381 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
    10441 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
    10501 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
    10561 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
    10621 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
    10681 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
    10741 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
    10801 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
    10861 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
    10921 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
    10981 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
    11041 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
    11101 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
    11161 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
    11221 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
    11281 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
    11341 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
    11401 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
    11461 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
    11521 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
    11581 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
    11641 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
    11701 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc
    11761 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
    11821 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
    11881 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac
    11941 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat
    12001 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
    12061 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
    12121 tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg
//
