LOCUS       V035389                10967 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V035389
VERSION     V035389
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2297..5719
                     /label="SREBF2(NM_004599)"
                     /note="SREBF2(NM_004599)"
                     /gene="SREBF2"
     promoter        5770..5981
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5994..6262
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6291..6300
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6321..6983
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     misc_feature    7062..7650
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7724..7957
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8029..8150
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8169..8304
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8342..8358)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8366..8382
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8390..8420)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8435..8456
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8744..9332)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9503..10363)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10364..10468)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10942..10958
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 tagcgaattc gccaccatgg acgacagcgg cgagctgggt ggtctggaga ccatggagac
     2341 cctcacggag ctgggcgacg agctgaccct gggagacatc gacgagatgc tgcaatttgt
     2401 cagtaatcaa gtgggagagt tccctgactt gttttcagaa cagctgtgta gctcctttcc
     2461 tggcagtggt ggtagtggta gcagcagcgg cagcagtggc agcagcagca gcagcagcaa
     2521 tggcaggggc agcagcagcg gagctgtgga cccttcagtg caacggtcat tcacccaggt
     2581 cacattacct tccttctctc cctcggcggc ctccccacag gctccaactc tgcaagtcaa
     2641 ggtttctccc acctcagttc ccaccacacc cagggcaact cctattcttc agccccgccc
     2701 ccagccccag cctcaacctc aaactcagct gcaacaacag acggtaatga tcacgccaac
     2761 attcagcacc actccgcaga cgaggatcat ccagcagcct ttgatatacc agaatgcagc
     2821 tactagcttt caagtccttc agcctcaagt ccaaagcctg gtgacatcct cccaggtaca
     2881 gccggtcacc attcagcagc aggtgcagac agtacaggcc cagcgggtgc tgacacaaac
     2941 ggccaatggc acgctgcaga cccttgcccc ggctacggtg cagacagttg ctgcgccaca
     3001 ggtgcagcag gtcccggtcc tggtccagcc tcagatcatc aagacagatt cccttgtttt
     3061 gaccacactg aagacagatg gcagccctgt tatggctgcg gtccagaacc cggccctcac
     3121 cgccctcacc acccctatcc agacggctgc ccttcaagta ccaaccctgg tgggcagcag
     3181 tgggaccatt ctgaccacaa tgcctgtaat gatggggcaa gagaaagtgc ccattaagca
     3241 ggtacctggg ggagtcaagc agcttgagcc ccccaaagaa ggagaaaggc ggacaaccca
     3301 taatatcatt gagaaacgat atcgctcctc catcaatgac aaaatcatcg aattgaaaga
     3361 cctggtcatg gggacagacg ccaagatgca caagtctggc gttctgagga aggccattga
     3421 ttacatcaaa tacttgcagc aggtcaatca taaactgcgc caggagaaca tggtgctgaa
     3481 gctggcaaat caaaagaaca agcttctaaa gggcatcgac ctaggcagtc tggtggacaa
     3541 tgaggtggac ctgaagatcg aggactttaa tcagaatgtc cttctgatgt cccccccagc
     3601 ctctgactca gggtcccagg ctggcttctc tccctactcc attgactctg agccaggaag
     3661 ccctctattg gatgatgcaa aggtcaaaga tgagccagac tctcctcctg tggcgctggg
     3721 catggtagac cgctcacgga ttcttctgtg tgtcctcacc ttcctgtgcc tctcctttaa
     3781 ccccctgact tccctgctgc agtggggagg ggcccacgac tctgaccagc acccacactc
     3841 aggctctggc cgcagtgtcc tgtcattcga gtcaggttct gggggctggt ttgactggat
     3901 gatgcctact cttctcttat ggctggtaaa tggtgtgatt gtcctgagcg tctttgtgaa
     3961 gctgctggtt catggggagc cagtgatccg gccacactcg cgctcctcgg tcaccttctg
     4021 gaggcaccgg aaacaggcag atctggatct cgccagagga gattttgcag ctgctgccgg
     4081 caacctacaa acctgcctgg cagttttggg ccgggcactg cccacctccc gcctggacct
     4141 ggcctgcagc ctctcctgga acgtgatccg ctacagcctg cagaagctac gcctggtgcg
     4201 ctggctgctc aagaaagtct tccagtgccg gcgggccacg ccagccactg aggcaggctt
     4261 tgaagacgaa gctaagacca gcgcccggga tgcggctctg gcctatcacc ggctgcacca
     4321 gctgcacatc acagggaagc ttcctgcagg atccgcctgt tccgatgtac acatggcgtt
     4381 gtgtgccgtg aacctggctg aatgtgcaga ggagaagatc ccaccgagca cactggttga
     4441 gatccatctg actgctgcca tggggctcaa gacccggtgt ggaggcaagc tgggcttcct
     4501 ggccagctac ttcctcagcc gagcccagag cctgtgtggc cccgagcaca gtgctgttcc
     4561 tgactccctg cgctggctct gccaccccct gggccagaag tttttcatgg agcggagctg
     4621 gtctgtgaag tcagctgcca aggagagtct atactgtgcc cagaggaacc cagctgaccc
     4681 cattgcgcag gtccaccagg ccttctgcaa gaacctgctg gagcgagcta tagagtcctt
     4741 ggtgaaacct caggccaaga agaaggctgg agaccaggaa gaagagagct gtgaattctc
     4801 cagtgctctg gagtacttga aattacttca ttcttttgtg gactctgtgg gggttatgag
     4861 ccccccactc tccaggagct ccgtgctcaa gtccgccctg ggtccagaca tcatctgtcg
     4921 gtggtggacg tctgcaatca ctgtggccat cagctggctc cagggagacg atgcagctgt
     4981 gcgctctcat tttaccaaag tggaacgcat ccccaaggcc ctggaagtga cagagagccc
     5041 cctggtgaag gccatcttcc atgcctgcag agccatgcat gcctcactcc ctgggaaagc
     5101 agatgggcag cagagttcct tctgccattg cgagagggcc agtggccacc tatggagcag
     5161 cctcaacgtc agtggggcca cctctgaccc tgccctcaac cacgtggtcc agctgctcac
     5221 ctgtgacctg ctactgtcgc tacggacagc gctctggcaa aaacaggcca gtgccagcca
     5281 ggctgtgggg gagacctacc acgcgtcagg cgctgaactg gcgggcttcc aacgggacct
     5341 gggcagcctg cgcaggctgg cacacagctt ccgcccagca taccgcaagg tgttcctgca
     5401 tgaagccacc gtgcgcctga tggcaggagc cagccccacc cgcacccacc agctgctgga
     5461 acacagcctg cggcggcgca ccacgcagag caccaagcac ggagaggtgg atgcctggcc
     5521 cggccagcga gagcgggcca ccgccatcct gctggcctgc cgccacctgc ccctctcctt
     5581 cctctcctcc ccgggccagc gggcagtgct gctggccgaa gctgcccgca ccctggagaa
     5641 ggtgggcgac cggcgctcct gcaacgactg ccagcagatg attgttaagc tgggtggtgg
     5701 cactgccatt gccgcctcct gaggatccgc ggccgcgaag gatctgcgat cgctccggtg
     5761 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg
     5821 gcaattgaac gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt
     5881 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg
     5941 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca gctgaagctt cgaggggctc
     6001 gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc cggttgagtc
     6061 gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt
     6121 taaagctcag gtcgagaccg ggcctttgtc cggcgctccc ttggagccta cctagactca
     6181 gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt
     6241 ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc tacgctagac gccaccatgg
     6301 agagcgacga gagcggcctg cccgccatgg agatcgagtg ccgcatcacc ggcaccctga
     6361 acggcgtgga gttcgagctg gtgggcggcg gagagggcac ccccaagcag ggccgcatga
     6421 ccaacaagat gaagagcacc aaaggcgccc tgaccttcag cccctacctg ctgagccacg
     6481 tgatgggcta cggcttctac cacttcggca cctaccccag cggctacgag aaccccttcc
     6541 tgcacgccat caacaacggc ggctacacca acacccgcat cgagaagtac gaggacggcg
     6601 gcgtgctgca cgtgagcttc agctaccgct acgaggccgg ccgcgtgatc ggcgacttca
     6661 aggtggtggg caccggcttc cccgaggaca gcgtgatctt caccgacaag atcatccgca
     6721 gcaacgccac cgtggagcac ctgcacccca tgggcgataa cgtgctggtg ggcagcttcg
     6781 cccgcacctt cagcctgcgc gacggcggct actacagctt cgtggtggac agccacatgc
     6841 acttcaagag cgccatccac cccagcatcc tgcagaacgg gggccccatg ttcgccttcc
     6901 gccgcgtgga ggagctgcac agcaacaccg agctgggcat cgtggagtac cagcacgcct
     6961 tcaagacccc catcgccttc gccagatccc gcgctcagtc gtccaattct gccgtggacg
     7021 gcaccgccgg acccggctcc accggatctc gctaagtcga caatcaacct ctggattaca
     7081 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     7141 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     7201 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     7261 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     7321 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     7381 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     7441 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     7501 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     7561 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     7621 gtcggatctc cctttgggcc gcctccccgc ctggtacctt taagaccaat gacttacaag
     7681 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     7741 tcccaacgaa aataagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     7801 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     7861 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     7921 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     7981 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
     8041 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     8101 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     8161 ctctagctat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     8221 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     8281 agtgaggagg cttttttgga ggcctagact tttgcagaga cggcccaaat tcgtaatcat
     8341 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     8401 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     8461 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     8521 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     8581 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     8641 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     8701 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     8761 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     8821 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     8881 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     8941 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     9001 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     9061 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     9121 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     9181 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     9241 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     9301 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     9361 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     9421 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     9481 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     9541 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     9601 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     9661 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     9721 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     9781 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     9841 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     9901 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     9961 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
    10021 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
    10081 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
    10141 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
    10201 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
    10261 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    10321 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    10381 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    10441 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
    10501 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
    10561 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
    10621 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
    10681 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
    10741 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
    10801 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
    10861 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
    10921 ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagctg
//
